Báo cáo khoa học: Structural flexibility in Trypanosoma brucei enolase revealed by X-ray crystallography and molecular dynamics pdf

... compilation ª 2007 FEBS 5089 Structural flexibility in Trypanosoma brucei enolase revealed by X-ray crystallography and molecular dynamics Marcos V. de A. S. Navarro 1, * ,‡ , Sandra M. Gomes Dias 1, * ,§ , ... of the inhibition of T. brucei enolase by FPAH. Fig. S1. Lineweaver–Burk plots for T. brucei enolase in the presence of FPAH. Structural flexi...

Ngày tải lên: 23/03/2014, 07:20

... doi:10.1046/j.1432-1033.2003.03399.x Molecular structural studies of LPS from H. in uenzae strains [9–19] have resulted in a structural model consisting of a conserved phosphoethanolamine (PEtn)-substituted triheptosyl inner-core ... ( D , D –Hep) [13]. In NTHi strains SB 33 and 176 [16,18], disubstitution by b- D -Glcp (at O-4) and PCho (at O-6) occurs, while in strain RM1...

Ngày tải lên: 08/03/2014, 08:20

... 6097–6100. Supplementary information The following supporting information is available: Fig. S1. Retrohoming in vivo of wild-type intron and C-terminal region mutant derivatives using RmInt1- engineering constructs ... Group II intron-encoded proteins are multifunctional and contain an N-terminal reverse transcriptase domain, followed by a putative RNA-binding domain (domain X) associ...

Ngày tải lên: 15/03/2014, 09:20

... shown) revealed the ordering of 394–410 loop residues and interactions with Gln400, despite replacing Trp396 with alanine. The in uence of mutations in the serine-binding pocket on magnesium binding The ... binding of ATP and the amino acid substrate in the active site are linked, and incorrect binding of ATP can affect serine binding and vice versa [22]. Asn435, together with...

Ngày tải lên: 16/03/2014, 06:20

... longest amino acid sequences that included the DNA binding, hinge and ligand-binding domains were used for this analysis, except for the Knirps fam- ily members that lack the LBD. In D. melanogaster, Table ... experiment (right), 5 day PV fat bodies were incubated in CM for 30 min and established as initial time point (0 h); then were incubated in CM or with 20E for 4 and 16 h, or...

Ngày tải lên: 18/02/2014, 13:20

... mitochondrial and chloroplastic targeting signals in terms of arginine and serine con- tent (i.e. not as high as in mitochondrial targeting signals) and may be slightly enriched in hydrophobic residues. In ... provided, and the insights that may be gained from a greater under- standing of the mechanism of dual targeting for the targeting of location-specific proteins is discuss...

Ngày tải lên: 07/03/2014, 03:20

... engineering, expression and purification of single-cysteine-containing proteins comprising either the C-terminal NBD or the N-terminal NBD as fusions to Escherichia coli maltose-binding protein ... of P-glycoprotein contains only a single cysteine in the Walker-A motif [43]. Thus, MBP-NBD-Nter is a 75-kDa fusion protein containing a single cysteine residue located in the ATP-binding pock...

Ngày tải lên: 08/03/2014, 08:20

... hypoxia may increase tran scription of the HO-1 gene through a certain protein factor in RPE cells. These results indicate that RPE cells maintain retinal homeostasis by repressing or inducing the ... urvival and maintenance of RPE in the adult retina. On the other hand, we have shown that HO-1 expression is reduced in human cell lines by treatmen t with interferon-c [26,27] or b...

Ngày tải lên: 23/03/2014, 13:20

... with the laminar organization revealed by negative staining and immunogold labelling [26]. A non-consensus sequence within GRINL1A is responsible for DYNLL1 binding in the canonical mode In this ... absolutely required for binding and is most likely involved in increasing binding affinity. G426 of GRINL1A occu- pies the equivalent position to this glutamine, which is typically in...

Ngày tải lên: 29/03/2014, 09:20

... containing HIT with three histidines separated by hydrophobic residues (His-u- His-u-His-u). Structural analysis has revealed that HIT proteins exist as homodimers containing nucleo- tide-binding ... these crucial amino acids resulted in enzyme inactivation or a significant decrease in activity [20]. Two amino acids, Asp205 and Lys207, are involved in interactions with the 2¢-O-...

Ngày tải lên: 29/03/2014, 09:20