... of the membrane. The other site, the Q D site (the distal Q -site) is located approximately 25 A ˚ from the Q P site on the opposite (periplasmic) side of the membrane [3,10]. The relatively large ... One site, the Q P site (the proximal Q -site) , is located in the interface region between the FrdCD subunits and the [3Fe-4S] cluster coordinating region of...
Ngày tải lên: 23/03/2014, 13:20
... H 4 biopterin. The sites were (a) a redox site, involved in the reduction of the active site iron [Fe(III) fi Fe (II) ], (b) a catalytic site, involved in the activation of dioxygen and hydroxylation of L-Phe, ... displacement of the cofactor bound at the active site by 2.6 A ˚ (mean distance) in the direction of the iron and Glu286 which causes a loss o...
Ngày tải lên: 17/03/2014, 09:20
Tài liệu Báo cáo khoa học: nsights into the reaction mechanism of glycosyl hydrolase family 49 Site-directed mutagenesis and substrate preference of isopullulanase doc
... located in the vicinity of the active site and could form a binding site for a glucosyl unit i n subsite )1 (Fig. 4B). Together with the observations from the site- directed mutagenesis, Trp402 of IPU ... polysaccharide, pullulan. Comparison of the active sites of the model of IPU and Dex49A The active site structures of t he model of IPU (Fig. 4A) an...
Ngày tải lên: 19/02/2014, 16:20
Tài liệu Báo cáo khoa học: "Methods for the Qualitative Evaluation of Lexical Association Measures" doc
... we find: (i) Examination of 50% of the data in the SLs leads to identification of between 75% (AdjN) and 80% (PNV) of the TPs. (ii) For the first 40% of the SLs, and lead to the worst results, with ... mixed; (ii) the grammatical status of the individual word combinations in the base set, i.e., whether they are part of or constitute a phrase or simply co- occur w...
Ngày tải lên: 20/02/2014, 18:20
Báo cáo khóa học: Insight into the activation mechanism of Bordetella pertussis adenylate cyclase by calmodulin using fluorescence spectroscopy pptx
... direct exploitation of the structural results of the former to explain the mechanism of CaM activation of the latter. Crystallization and resolution of the 3D structures of AC and AC–CaM, which ... coupling Fig. 5. Binding of Ant-dATP to AC (d) and AC–CaM (m). The degree of binding of the ligand was calculated from the value of the ampli- tude of the...
Ngày tải lên: 07/03/2014, 15:20
Báo cáo khoa học: Bacitracin inhibits the reductive activity of protein disulfide isomerase by disulfide bond formation with free cysteines in the substrate-binding domain pptx
... 10 mm). The structural identity of the isolated analogs was con- firmed by MALDI-TOF ⁄ TOF MS, with a 4800 MALDI- TOF ⁄ TOF Analyzer (AB SCIEX, Framingham, MA, USA). MALDI-TOF and TOF ⁄ TOF MS spectra ... ring of bacitracin A (937.33). Negative ion mode CID-TOF ⁄ TOF MS analysis of the same precursor yielded the corresponding signature peaks (m ⁄ z 1404.88, 1438.82 and 1470.80)...
Ngày tải lên: 14/03/2014, 23:20
Báo cáo khoa học: Intrabodies against the EVH1 domain of Wiskott–Aldrich syndrome protein inhibit T cell receptor signaling in transgenic mice T cells potx
... region. The association of WASP and the Arp2 ⁄ 3 complex activates the actin nucleation activity of the Arp2 ⁄ 3 complex [23]. To investigate further the function of the WASP- EVH1 domain in the ... for the target molecules. However, the cloning of heavy and light chain variable regions by RT-PCR can be difficult because of the presence of nonspecific variable...
Ngày tải lên: 16/03/2014, 14:20
Báo cáo khoa học: Principles behind the multifarious control of signal transduction ERK phosphorylation and kinase/phosphatase control potx
... are controlled by the kinases or phosphatases in the system, and whether they are controlled differ- entially by some or all of them. On the basis of the antagonistic action of the kinases and ... how the second kinase in the cascade determines the time dependence of the activity of the third kinase, we varied the V max of the second kinase reaction and...
Ngày tải lên: 16/03/2014, 18:20
Báo cáo khoa học: Mapping contacts between regulatory domains of skeletal muscle TnC and TnI by analyses of single-chain chimeras potx
... locate the portion corresponding to residues 148–182 of TnI. Table 2. The ratios of the volumes (V) of the models built by SAXS and of the structure of TnC(1–91) (1SKT) are compared with their molecular ... (Fig. 4A,B), where the maximum of the curves had approximately the same value in both cases and by the Kratky plot that showed similarity in the flexibil...
Ngày tải lên: 16/03/2014, 18:20
Báo cáo khoa học: "Search in the Lost Sense of “Query”: Question Formulation in Web Search Queries and its Temporal Change" pptx
... clustering but on understanding the expression of intent. 3 Method We address the main thesis of the work by retrospec- tively studying queries issued to a search engine over the course of 12 consecutive months. Q-queries. ... for by Q-queries. We plot this against b’s activity level, measured by the number of queries issued by b in a month. We binned users by thei...
Ngày tải lên: 17/03/2014, 00:20