Báo cáo khoa học: Modulation of heme and myristate binding to human serum albumin by anti-HIV drugs An optical and NMR spectroscopic study potx

... Allosteric modulation of myristate and Mn(III) heme binding to human serum albumin. Optical and NMR spectroscopy characterization. FEBS J 272, 4672–4683. 37 Fanali G, Ascenzi P & Fasano M (2007) ... binding by anti-HIV drugs G. Fanali et al. 4502 FEBS Journal 274 (2007) 4491–4502 ª 2007 The Authors Journal compilation ª 2007 FEBS Modulation of heme...

Ngày tải lên: 23/03/2014, 07:20

... approach to understand the inhibitory process between a serpin and an enzyme [11–13] and to develop specific inhibitors. Several serpins, such as protein C inhibitor, a2-antiplas- min, antithrombin-III, ... (Laval University, Canada). Human chymotrypsin (Chtr), urokin- ase plasminogen activator (uPA), human kallikrein hK1, human plasma kallikrein (PK), human neutrophil elasta...

Ngày tải lên: 30/03/2014, 13:20

... and C) and MPP + (B and D) were analysed by SDS ⁄ PAGE (A and B) on 5–15% crosslinked polyacrylamide gel and western blotting (C and D). (A, B) Lane M, prestained molecular mass mark- ers; lane ... of this study was to delineate any direct role of MPTP in the aggregation of a-synuclein and the effect of dopamine on this process. Toxicity of MPTP is believed to...

Ngày tải lên: 14/02/2014, 19:20

... homeostasis are conserved between yeasts and humans; and ergos- terol, the predominant sterol of yeast, is structurally and functionally related to sterols of higher eukary- otes [31]. Ergosterol ... onto medium lacking histi- dine, leucine and uracil to monitor protein interactions. The unre- lated genes STE14 and UBC6 served as negative controls. Sterol homeostasis modula...

Ngày tải lên: 18/02/2014, 13:20

... the time-course modulation of foreign gene expression and to investigate whether this modulation can be explained by the concomitant changes occurring in the parasite genome organization. Results and Discussion Expression ... and lanes 5–14 corres- pond to the assay carried out with DNA samples obtained from transformed parasites after 2 days and 2, 4, 6 and 24 weeks aft...

Ngày tải lên: 19/02/2014, 07:20

... 271) 4465 Modulation of cyclin D1 synthesis and hyperphosphorylation of Rb by n-3 and n-6 PUFAs Induction of cyclin D1 is one of the e arliest effects of mitogenic factors lea ding to cell c ... 4463 phy films were scanned and quantified by densitometry by using QUANTITY ONE software (Bio-Rad, Hercules, CA, USA). Real-time quantitative RT-PCR Quantitative RT-PCR was pe...

Ngày tải lên: 19/02/2014, 16:20

... compare histo- grams, and lane 2 with lane 8). Discussion Autoregulation of GRIP1 transactivation activity To date, some of the functions of the N- and C-ter- mini of p160 co-activators were unclear. ... the modulation of self-transactivation (AD1 and AD2) and co-activator (AR, ER and TR) functions in HeLa cells. The outcome of the relationship between GRIP1 transact...

Ngày tải lên: 07/03/2014, 12:20

... concentrations of the TOP1 inhibitor, camptothecin, in the presence and in the absence of 10 )5 m MTX. The cytotoxic effect of camptothecin alone was enhanced by the addition of the concentration of MTX to ... developed and even now con- tinues to play an important role in the chemotherapy of human malignancies such as acute lymphoblastic leukemia, lymphoma, osteosar...

Ngày tải lên: 07/03/2014, 16:20

... McLaurin 2,3 1 Department of Chemistry, University of Toronto, Canada 2 Centre for Research in Neurodegenerative Diseases, University of Toronto, Canada 3 Department of Laboratory Medicine and Pathobiology, ... epi-2-inosose and allo-inositol. These results suggest that epi-2-inos- ose and allo-inositol are either inhibitors of oligomer formation or inhibitors of both oligom...

Ngày tải lên: 16/03/2014, 06:20

... Sur- viving clones were then expanded in the same medium and analyzed for the expression of TRPM4b by RT-PCR and western blotting. RT-PCR Total RNA was isolated, and RT-PCR was performed as described ... of TRPM4b expression on Ca 2+ entry and changes of the transmembrane poten- tial, HEK-293 clones stably expressing TRPM4b were generated. Clones derived by selection for neo...

Ngày tải lên: 23/03/2014, 09:21