Báo cáo khoa học: Mycobacterium tuberculosis ClpC1 Characterization and role of the N-terminal domain in its function ppt

... additional N -domain, homologous to the N-domains of ClpA or ClpB, and a linker domain homologous to, but half the size of, the linker domain of ClpB [19]. The N-terminal region contains two 32-amino acid ... action of these proteins in M. tuberculosis. In this study, we functionally characterized the ClpC1 protein of M. tuberculosis, and investigated the...

Ngày tải lên: 23/03/2014, 06:20

... organisms and the evolution of vitamin C biosynthetic pathways in general. What could be the physiologic role of the Rv1771 protein in M. tuberculosis? Mycobacterium tuberculosis has coevolved with its ... Escherichia coli. The recombinant protein contained an engineered enterokinase cleavage site in the junction between the GST tag and the Rv1771 sequence...

Ngày tải lên: 07/03/2014, 12:20

... observed in SDS/PAGE and in 2D PAGE (Figs 1 and 2; Table 1). The N-terminal amino acid sequences (see above) allow the assignment of the HcA clone to the major sequence of the upper hemocyanin band in ... raised against the hemocyanin fraction show staining of the two 75 kDa bands in Western blot, but do not recognize any other proteins of the hemolymph....

Ngày tải lên: 20/02/2014, 11:20

... 7). Discussion The aim of the present study was to investigate the intracellular fate of PrP C by examining for the first time its biosynthesis in the secretory pathway of neuro- endocrine cells in vivo and ... suggesting that the level of GFP–PrP C transgene expression was dependent on the colour of the background of the animal. The fusion protein was f...

Ngày tải lên: 16/03/2014, 14:20

... transition of ESAT-6 and the complex monit- ored in the far-UV CD region at 222 nm. Thermal unfolding (h) and thermal refolding (s) profile of ESAT-6 and thermal unfolding (n) and thermal refolding (e) ... isotherm in Fig. 1B was fitted to a single-site binding model for determination of thermodynamic parameters. The solid line indicates best fit to the plot. The para...

Ngày tải lên: 30/03/2014, 11:20

... chromatin assembly occurring during S phase of the cell cycle [9]. While the ordered helical domains in the globular body of the core histones provide a structure for DNA to wrap around [10], the N-terminal ... with the topology changes resulting from the loss of 1.5 superhelical turns of the DNA over the whole construct, suggest that the chromatin is indeed mo...

Ngày tải lên: 19/02/2014, 12:20

... 3.1.4 Generating distance feature. In the final stage of preparing training data, we add the distance, i.e. number of words, between a Chinese token feature and the English term in question, ... training and testing. We obtained a total of 155,310 article pairs, from which we then randomly selected 13,150 and 2,181 titles as seeds to obtain the training and te...

Ngày tải lên: 19/02/2014, 19:20

... an Exportin-5-dependent mechanism, and further processed by the RNAse III enzyme Dicer, producing miRNA duplexes. One strand of the duplex is incorporated into the RNA-induced silencing complex, ... hampers miR-143 pro- cessing in a transcription-independent manner. These results are once again in agreement with the results obtained in SW480 cells. In these cells expressin...

Ngày tải lên: 07/03/2014, 00:20

... first coding exon, and thereby contains a unique N-termi- nal domain but shares the same six zinc finger DNA-binding domains as present in SAF-1. In addition, a negatively functioning domain present ... (b) binding of the N-terminus by ancillary factors, (c) the lack of a negatively functioning module that is present in the N-terminus of SAF-1 [26], or (d) all of th...

Ngày tải lên: 07/03/2014, 02:20

... preserved in their original ordering. For training and evaluation purposes, we used a random split of the collection into 90% training and 10% test. This yields a training corpus of 259 docu- ments and ... eliminate the inversions, which are typical in human-built indexes. Inversion is a method used by professional indexers by which they break the order- ing of the wor...

Ngày tải lên: 08/03/2014, 01:20