Báo cáo khoa học: Hydrogen bond residue positioning in the 599–611 loop of thimet oligopeptidase is required for substrate selection pdf

... to other substrates, the bradykinin derivative is also unaffected by Gly mutations in the loop. This distinction suggests that the role of Gly residues in the loop is to properly orientate these ... among the four substrates tested. This discovery helped reveal the primary role of Gly residues in the 599–611 loop in positioning the Tyr605 and Tyr612 re...

Ngày tải lên: 23/03/2014, 06:20

... requires the cooperation of TTQ biogenesis genes from the mau gene cluster. By performing detailed kinetic studies of both AADH enzymes, we show that the recombinant enzyme is indistinguishable from the ... owing to the poor buffering capa- city of BisTris propane at this pH. Analysis of the data (omitting the pH 10 data point) using the equa- tion for a double ion...

Ngày tải lên: 19/02/2014, 07:20

... function of the methyl group may involved improved orientation of the histidine inside the coenzyme B-binding site. With the methylation, the histidine residue is prevented from forming two alter- nate ... hydrogen bonds of the protein to the phosphate group of coenzyme B. The distance of 2.65 A ˚ indicates the presence of a strong hydrogen bond with fu...

Ngày tải lên: 16/03/2014, 05:20

... v 2 of Phe298 is about )30°. In both conformations the uncharged side-chain of Lys296 is hydrogen bonded with the O4 of TPQ. To determine the charged-state of residues involved in substrate binding ... of the substrate. The amino group in the substrate tail, which is positively charged in the pH range explored, may facilitate the correct positio...

Ngày tải lên: 06/03/2014, 00:21

... that hydrogen bonding between the imidazole nitrogen of the conserved His and the hydroxyl of the active-site Ser increases the nucleophilicity of the Ser oxygen, facilitating attack of the hydroxy ... This reac- tion is facilitated by hydrogen bonding between the imidazole nitro- gen of the conserved histidine (His) and the hydroxyl of the Ser. W. E. G...

Ngày tải lên: 18/02/2014, 16:20

... P2Y 12 - dependent part of the Ca 2+ response, indicating that PI3-Kß is the main isoform in Ca 2+ signal modulation via P2Y 12 . Examination of targets downstream of AC BD Fig. 10. PI3-K ß-isoform mediates ... considerable amount of the PIP 3 formed in plate- lets [21], this may suggest that the PIP 3 produced by this isoform leads to plasma membrane binding and, hence,...

Ngày tải lên: 18/02/2014, 16:20

... ProDom, C−terminus Length of the N terminus in % of the deleted domain Length of the C terminus in % of the deleted domain Pfam, N−terminus Pfam, C −terminus Number of occurences Number of occurences 25000 35000 55000 0 ... termini, depending on the number of domains in the protein and the database selected (Fig. 1). The prevalence of terminal deletions d...

Ngày tải lên: 19/02/2014, 07:20

... absorption of vitamin B 12 facilitating its internalization [2,3,5]. Lack or malfunction of this Cbl binder hampers the uptake of the minute amounts of the vitamin present in food. Only around 1% of the ... source of human IF for analytical and therapeutic purposes. Keywords: arabidopsis; cobalamin; intrinsic factor; recom- binant. Vitamin B 12 (cobalamin, Cbl) is t...

Ngày tải lên: 21/02/2014, 00:20

... modern era, yet existing medicines to combat this global pandemic are disappointingly limited in terms of safety and effectiveness. The inadequacy of currently available therapies for obesity has ... suggesting that these cyclin-dependent kinase inhibitors are important in the regulation of adipocyte number. The S-phase kinase-associated protein (Skp)1–Cullin- F-box protein...

Ngày tải lên: 06/03/2014, 01:20

... novo lipid synthesis. The PI3K ⁄ Akt signaling pathway is best known for its role in the maintenance of cell survival, but is also responsible for the up-regulation of glucose metabo- lism and the induction ... resulted in the disappearance of LDs (Fig. 2), suggesting that these cells carried out lipogenesis (de novo synthesis of lipids) in control cultures. In...

Ngày tải lên: 07/03/2014, 00:20