Báo cáo khoa học: Expression and characterization of the biofilm-related and carnosine-hydrolyzing aminoacylhistidine dipeptidase from Vibrio alginolyticus pot

... electrophoresis. Another striking difference of the MALDI-TOF spec- tra from the modified Fe-protein in comparison to the unmodified Fe-protein is the decreased intensity of peak 1625.4 m ⁄ z and the absence of ... inactivation of the enzyme. Thus, the results obtained in the pres- ent study extend our knowledge of the nitrogenase post-translational modification s...

Ngày tải lên: 07/03/2014, 02:20

... designates the N-terminal transmembrane helix of the protein. On the basis of the three sets of secondary shift data in Fig. 3 (right), the helices probably range from I39 to E48 (helix 2) and from ... probes 16-doxylstearic acid in the interior of the micelle (A) and Mn 2+ in the aqueous buffer (B) selectively attenuate distinct regions of VpU- cyt, reflectin...

Ngày tải lên: 18/02/2014, 06:20

... in terms of the number of the enzymes involved, the kinetic mechanisms of the competing enzymes and the number as well as the nature of the allosteric controls. Whether such diversity in the organization ... consequence of the time delay in the enzymatic chain. Subtraction of the rate of the cystathionine b-lyase side reaction from the total rate o...

Ngày tải lên: 20/02/2014, 02:21

... USA) at the ÔServeis Cientifico-TecnicsÕ of the University of Barcelona, using the 488 nm line of an argon laser and standard optical emission filters. Percentages of cells at each phase of the cell cycle ... to verify that the relatively low uptake of WP631 was at the origin of the low apoptosis, and whether longer periods of treatment could cause significant a...

Ngày tải lên: 20/02/2014, 23:20

... Additionally, because the demand of the chloroplast is ‘sensed’ inside the organelle, the IEM is closest to the origin of the signal, and thus regulation at the Tic complex could be one of the fastest ... transmembrane-helices at the extreme N-terminus, which anchor the protein in the membrane. The position and function of the long C-terminal tail on the...

Ngày tải lên: 07/03/2014, 03:20

... YGNGVXC motif in the N-terminus, with the cysteine involved in a disulfide bridge, and are otherwise unmodified except for cleavage of the leader from their precursor (Table 1). They show potent activity against ... report the chemical synthesis, bio- chemical production, and solution structure of preCbnB2, and compare it with the structure of the mature bacteriocin, C...

Ngày tải lên: 07/03/2014, 15:20

... a manually-assembled, and therefore high-precision, set of representative instances of the class. To evaluate the precision of the extracted in- stances, the manual label of each gold-standard class (e.g., ... identification of a noisy pool of candidate at- tributes, as remainders of queries that also contain one of the class instances. In the case of the clas...

Ngày tải lên: 08/03/2014, 01:20

... donor and acceptor, this result matches with the results from the X-ray crystallography of the VBC complex [26]; the distance between the C-terminal end of elongin B and the N-terminal end of elongin ... between other combinations of termi- nal ends of elongin B and elongin C, although the effects caused by the binding of pVHL on the 3D struc- ture of...

Ngày tải lên: 16/03/2014, 05:20

... (weight of water ⁄ weight of protein). The ability of organic solvents to ‘strip off’ water from the enzyme surface was clearly dependent on the nature of the organic solvent. The rmsd of the enzyme ... and benzene) water is preferentially localized in the most polar regions of the enzyme. The majority of the enzyme surface is in direct contact with the...

Ngày tải lên: 16/03/2014, 10:20

... performed assays with the Arabidopsis enzymes in the absence of one of each of the nonenzymatic components. Compar- able to previous reports, we found that the synthesis by GER1 of GDP-Fuc from the intermediate ... by NMR and by a functional assay. Results Cloning and expression of Caenorhabditis and Drosophila GMD and GER cDNAs Homologues of the human GMD...

Ngày tải lên: 16/03/2014, 13:20