Báo cáo khoa học: Characterization of inhibitory mechanism and antifungal activity between group-1 and group-2 phytocystatins from taro (Colocasia esculenta) pdf

... Journal compilation ª 2008 FEBS 4989 Characterization of inhibitory mechanism and antifungal activity between group-1 and group-2 phytocystatins from taro (Colocasia esculenta) Ke-Ming Wang 1 , Senthil ... peptide (group-2) (Fig. 2B). Although antifungal activity of phytocystatins from taro, strawberry and chestnut has been reported previ- ously...

Ngày tải lên: 23/03/2014, 06:20

... Sigma-Aldrich and Carlo Erba (Milan, Italy), and were of the highest available grade. Acknowledgements Thanks are due to Prof G.L. Sottocasa and Dr Anto- nella Bandiera (University of Trieste) ... – University of Trieste) for the immunization and bleeding of rabbits; to Silvia Zezlina for the affin- ity purification of antibody A from rabbit sera; to Dr Paolo Ermacora and...

Ngày tải lên: 20/02/2014, 01:20

... r an ging from 5.9 to 6 .1 (Fig. 3). Fractions e and f have at leas t one unique isoform e ach ( pI 5.9 for e and pI 6 .1 for f), while two bands of pI 5.95 and pI 6.0werecommontoeachofthemajorCLAPfractions, although ... o temperatures and pH normally inconsistent with CL activity. Primary sequence analysis of CLAP and cDNA clones coding for CLAP s how it to be a cell adhesio...

Ngày tải lên: 07/03/2014, 16:20

... level activity (100 %) and compared with the activity of mutated enzyme StkPK42R (lane 2) and StkP in the presence 0.1 m M,1mM and 10 mM of staurosporine (lanes 3, 4, and 5, respectively), a ... of RNAP and S1 protein has already been described in E. coli and resulted in significant stimulation of the RNAP transcriptional activity from a number of pro- moters in v...

Ngày tải lên: 16/03/2014, 18:20

... Burgering 3 and Lech Trzeciak 1 1 Department of Molecular Biology, International Institute of Molecular and Cell Biology in Warsaw, Poland 2 Laboratory of Molecular Medicine, Institute of Biochemistry and ... Ser166 and Thr168 located within a T-loop play a significant role in the regulation of TSSK3 activity and suggest a similar mechanism of activation to that of...

Ngày tải lên: 23/03/2014, 11:20

... was a result of decreased H vALP protein l evels, we compared HvALP from YHD2, YDK, and larvae from the F1 generation of backcrosses between YDK and YHD2 adults, using immunodetection and alkaline ... membrane-bound form of alkaline phosphatase from B. mori recognized HvALP in BBMV from YDK, YHD2 and F 1 larvae. No d ifferences in intensity of recognition were observ...

Ngày tải lên: 23/03/2014, 13:20

... and 0.1% (v ⁄ v) trifluoroacetic acid). The concentration of solvent B was increased linearly from 15 to 23% during a time-period of 25 min, from 23 to 70% during a time-period of 45 min and from ... and 0.1% (v ⁄ v) trifluoroacetic acid]. The concentration of solvent B was increased linearly from 15 to 23% during a time-period of 25 min, from 23 to 70% during a time-p...

Ngày tải lên: 30/03/2014, 20:20

... Carmona Karaoglanovic 2 , Liliane Schoofs 1 , Arnold De Loof 1 and Roger Huybrechts 1 1 Zoological Institute of the Catholic University of Leuven, Laboratory of Developmental Physiology and Molecular Biology, ... (yp3) of the bluebottle fly Calliphora vicina, yp3 and yp2 of the housefly Musca domestica and yp1 of Table 1. Purification procedure of several ACE-competitive pe...

Ngày tải lên: 24/03/2014, 03:21

... PC and increased GPC, indicating an imbal- ance between PL synthesis and degradation and, con- sequently, an increase of TG. Other studies suggest that the simultaneous accumulation of ML and ... extracted lipids (A) and PCA extracts (A¢) from one representative sample of MCF-7 cells; 2D 1 H NMR spectra of extracted lipids (B) and PCA extracts (B¢) from one representa...

Ngày tải lên: 18/02/2014, 13:20

... origin and a high con- servation of this class of proteins at both the structural and functional levels. Given the multiplicity of CLPs in humans and insects [14], we hypothesized that homologues of ... sequence of about 90 amino acids of unknown function. The high degree of identity of the Cg-Clp1 and Cg-Clp2 Glyco_18 domains (84% iden- tity) argues for a conservation...

Ngày tải lên: 19/02/2014, 00:20