Báo cáo khoa học: Inducible knockout mutagenesis reveals compensatory mechanisms elicited by constitutive BK channel deficiency in overactive murine bladder pot

... Conditional versus constitutive BK channel ablation Inducible knockout mutagenesis reveals compensatory mechanisms elicited by constitutive BK channel deficiency in overactive murine bladder Franz ... channel in the regula- tion of urinary bladder function. However, the findings in this constitutive BK channel knockout model do not exclude t...

Ngày tải lên: 23/03/2014, 06:20

... 2; RIG-I, retinoic acid -inducible gene I; siRNA, small interfering RNA; TBK, Tank-binding kinase 1; TLR, Toll-like receptor; TRIF, Toll-IL1 receptor domain-containing adaptor inducing IFN-b. FEBS ... glycoproteins. This response initiates signaling pathways leading to the induction of protective cellular genes, including type-I interferons [initially interferon (IFN)-b, and then IFN- a] and...

Ngày tải lên: 18/02/2014, 16:20

... machinery is pre-existing in the cells and deadenylates IFN-b mRNA as soon as its synthesis is induced by stimulating agents. The fact that the 65-kDa protein binds ARE and CRID in UV-crosslinking ... HTB-113) were maintained in DMEM containing 10% of fetal bovine serum (FBS; Myoclone Super Plus, Life Technologies) and 1% of penicillin/ streptomycin. The cells were infected by addit...

Ngày tải lên: 17/03/2014, 10:20

... This finding demonstrates again that the specific interaction between an amino acid at a distinct position in the substrate protein and elements in the enzyme’s peptide-binding site will induce ... elements in the enzyme’s peptide-binding site. This specific interaction probably induces the changes in the structure of the peptide-binding site that cause the alterations in the permitted...

Ngày tải lên: 16/03/2014, 16:20

... 50 amino acids on the C-terminal side of a 30-residue motif previously recognized as involved in binding thiamin diphosphate (ThDP) in all ThDP-dependent enzymes. The role of individual residues in ... this set of conserved amino acids in the E1a chain was investigated by means of site- directed mutagenesis. We propose that particular residues are involved in: (a) binding the 2-ox...

Ngày tải lên: 20/02/2014, 23:20

... development in plants. Sev- eral recent discoveries in auxin biology, including the identification of F-box proteins as auxin receptors, have contributed to our understanding of the molecu- lar mechanisms ... 2009) doi:10.1111/j.1742-4658.2009.07033.x Auxin in uences growth and development in plants by altering gene expression. Many auxin-responsive genes have been characterized in...

Ngày tải lên: 07/03/2014, 01:20

... docking. The putative substrate-binding pocket is connected to the protein surface by a main channel providing direct access to the re-side of the isoalloxazine ring, near two histidine side chains ... active site (substrate-binding pocket) of AAO by molecular docking. The enzyme consists of two domains, the FAD-binding domain (bottom part) and the substrate- binding domain (top part),...

Ngày tải lên: 07/03/2014, 11:20

... are templated by their environment in the protein, being bound by five amino acid side chains arising within the four-helix bundles (Fig. 1). A l 1,3 -bridging glutamate carboxylate (Glu66, LPC numbering) ... reflected in the spectroscopic splittings. Both major and minor conformations of the two coordinating glutamate side chains lead to distinct coordination numbers for the two mangan...

Ngày tải lên: 08/03/2014, 08:20

... protein) were incubated for 3 min with or without 5 lm CdCl 2 or 5 lm CuCl 2 in the assay medium containing 110 mm KCl, 20 mm Tris, 5 mm KH 2 PO 4 ,50mm creatine, an excess of creatine kinase ... of modularizing the system of interest may allow a more resolved picture. However, this is often limited by the feasibility of assigning modules that interact via a single connecting intermediate...

Ngày tải lên: 16/03/2014, 02:20

... ainly c ause d by t he faster unfolding resulting from destabilization of the native state relative to the transition state, underlining the predominant importance of this region for maintaining ... DDG [D]50% and DDG # U 25  C shows that the decrease in the thermodynamic stability is mainly caused by an increase in the unfolding rate constant, i.e. a decrease in the kinetic stabil...

Ngày tải lên: 16/03/2014, 18:20