Báo cáo khoa học: Biochemical analysis of the human DMC1-I37N polymorphism potx

... members of the dynamin family, GBPs are classified as a part of the dynamin superfamily. Although their biochemical characteristics are well understood, there is only an incomplete picture of the ... con- served in the primary structure of the GTPase domain of hGBP5, so the formation of GMP must be impaired in an as yet unknown manner. Sequence alignments of the l...

Ngày tải lên: 15/03/2014, 10:20

... amplitudes and rate constants of the two other phases. The above should be gov- erned by the balance between the rate of reduction and oxidation of the acceptor side of PSII by the avail- able electron ... half-time of the J–I phase were both decreased with DCMU, demonstrating that the J–I rise is not directly related to the reduction of the PQ pool. A further...

Ngày tải lên: 19/02/2014, 05:20

... with BMAL1 and the bin ding to the E-box. Substitution of Arg65 for Ala abolished the interaction of DEC1 with BMAL1 and the binding of DEC1 to the E-box. However, substitution of His57 for Ala ... increase in the mutant mice, the disruption of DEC1 might be compensated by DEC2. Further investigation is required to clarify the functions of DEC1 and DEC2. The supp...

Ngày tải lên: 19/02/2014, 16:20

... calculation of the fraction (f a ) and the effective quenching constant (K Q ) for the most accessible class of Trp chromophores [16]. The pH dependence of the Trp fluorescence was studied in the pH ... exposure of the Trp chromophores to the solvent, the differences in these values at the two pH values, most likely reflect a pH-related conformational change of th...

Ngày tải lên: 21/02/2014, 00:20

... sheet with the b12 of the other monomer, and towards the C-terminus, the subsequent residues, Phe182–Lys186, pass through the a2 vicinity of the other monomer. For the FeR dimer, the N-terminal ... of the structure of NADP + -bound FeR revealed that the nicotinamide moiety of NADP + faces the re-side of the isoalloxazine ring, and that the 2¢-P-AMP moiety...

Ngày tải lên: 07/03/2014, 02:20

... indicates the elution positions of molecular mass markers are shown above the panels. The profiles of the three proteins overlap over a region that coincides with the expected molecular mass of the ... and binding of the third protein without displacing the one that was previously associated with the complex was interpreted as an evidence of simultaneous binding to...

Ngày tải lên: 07/03/2014, 05:20

... incomplete process- ing of the signal peptide was also reported [16,17]. The uncleaved part of the a-mating sequence at the N-ter- minus as well as the histidine tag at the C-terminus of the recombinant ... strain X-33 with the addition of a signal sequence for secretion of the recombinant pro- tein into the medium. Approximately 12 mg of the recombinant lect...

Ngày tải lên: 14/03/2014, 23:20

... introducing features of the consensus motif into the Zm-p60.1 scaffold, an analysis of structural and catalytic prop- erties of the mutants, and simulations of the sub- strate–enzyme interactions of the wild-type ... F200K and P2. Furthermore, the F193A and W373K muta- tions do not result in any change in the thermostability of the enzyme (Table S2), and thermal...

Ngày tải lên: 16/03/2014, 04:20

... properties of SPC4. The absorption spectrum of purified SPC4 was recorded in 50 m M sodium acetate pH 5. The inset shows the spectrum of the extract- ed heme. (B) MALDI-TOF-MS analysis of SPC4 heme. Characterization ... and also with the calculated mass based on the primary structure (Table 2). The mass of the sugar moiety in HRP C is therefore 9782 Da, correspondin...

Ngày tải lên: 16/03/2014, 13:20

... selectivity of the A-domains [9] simultaneously determines the primary sequence of the product. Subsequently the a ctivated amino acid i s tethered to the terminal thiol moiety of a 4¢-phosphopantetheinyl (Ppant) ... further efforts were made to track down the actual target of the inhibitors. DISCUSSION The purpose of t his work was to identify the key residues in...

Ngày tải lên: 17/03/2014, 11:20