Báo cáo khoa học: Biochemical analysis of the human DMC1-I37N polymorphism potx

Báo cáo khoa học: Biochemical properties of the human guanylate binding protein 5 and a tumor-speciﬁc truncated splice variant Mark Wehner and Christian Herrmann doc

... members of the dynamin family, GBPs are classiﬁed as a part of the dynamin superfamily. Although their biochemical characteristics are well understood, there is only anincomplete picture of the ... con-served in the primary structure of the GTPase domain of hGBP5, so the formation of GMP must be impairedin an as yet unknown manner. Sequence alignments of the large GTP-binding domains of hGBP1, ... for the lack of GMP formation(see Fig. S2). The similar hydrolytic activities of the a ⁄ b and C-terminally truncated forms of hGBP5 are incontrast to that of the analogous deletion mutant of hGBP1...

Tài liệu Báo cáo khoa học: Quantitative analysis of the experimental O–J–I–P chlorophyll ﬂuorescence induction kinetics Apparent activation energy and origin of each kinetic step Steve Boisvert, David Joly and Robert Carpentier doc

... amplitudes and rate constants of the two other phases. The above should be gov-erned by the balance between the rate of reductionand oxidation of the acceptor side of PSII by the avail-able electron ... half-time of the J–I phase were bothdecreased with DCMU, demonstrating that the J–I riseis not directly related to the reduction of the PQ pool.A further analysis of the inﬂuence of PQ reductionon ... when the temperature is raised. The effect of DCMU on the traces was to increase the amplitude of step J with the concurrent decline of stepI, and to retard the rise to Fm. With dPQ, the J...

Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt

... with BMAL1 and the bin ding to the E-box.Substitution of Arg65 for Ala abolished the interaction of DEC1 with BMAL1 and the binding of DEC1 to the E-box.However, substitution of His57 for Ala ... increase in the mutantmice, the disruption of DEC1 might be compensated byDEC2. Further investigation is required to clarify the functions of DEC1 and DEC2. The suppressive activities of DEC1 and ... connected to the DEC1 E-boxes(Fig. 2), while deletion of the Orange domain ( DEC1DOr-ange) had no effect on the suppressive activity, indicating the importance of t he bHLH region of DEC1. The HXXXXXXXR...

Tài liệu Báo cáo khoa học: Fluorescence analysis of the Hansenula polymorpha peroxisomal targeting signal-1 receptor, Pex5p pdf

... calculation of the fraction (fa) and the effectivequenching constant (KQ) for the most accessible class of Trp chromophores [16]. The pH dependence of the Trp ﬂuorescence was studiedin the pH ... exposure of the Trp chromophores to the solvent, the differences in these values at the two pH values,most likely reﬂect a pH-related conformational change of the protein. Lowering the pH to ... investigate the binding of the dansylated PTS1 peptideL2 to HpPex5p and correlate the ﬂuorescence data withstructural data, a molecular model of the HpPex5p wasbuilt based on the known structure of the...

Báo cáo khoa học: Structure analysis of the ﬂavoredoxin from Desulfovibrio vulgaris Miyazaki F reveals key residues that discriminate the functions and properties of the ﬂavin reductase family pdf

... sheet with the b12 of the other monomer, and towards the C-terminus, the subsequent residues, Phe182–Lys186, pass through the a2 vicinity of the other monomer. For the FeR dimer, the N-terminal ... of the structure of NADP+-bound FeRrevealed that the nicotinamide moiety of NADP+faces the re-side of the isoalloxazine ring, and that the 2¢-P-AMP moiety is held in the groove between the ... potential of recombinant ﬂavoredoxinFigure 4 shows the results of linear regression analysis of the logarithms for the redox ratio of the mediatorversus that of recombinant ﬂavoredoxin. The redoxpotential...

Báo cáo khoa học: Interaction analysis of the heterotrimer formed by the phosphatase 2A catalytic subunit, a4 and the mammalian ortholog of yeast Tip41 (TIPRL) ppt

... indicates the elution positions of molecular mass markers are shownabove the panels. The proﬁles of the three proteins overlap over a region that coincides with the expected molecular mass of the ... andbinding of the third protein without displacing the onethat was previously associated with the complex wasinterpreted as an evidence of simultaneous binding toPP2Aca. The results of these in ... beendescribed in vivo, the prevalent form of the PP2Aholoenzyme is the trimeric A:B:C complex. The num-ber of B-type subunits is still growing with newmembers continuously being discovered. The subunitcomposition...

Báo cáo khoa học: Expression analysis of the nucleocytoplasmic lectin ‘Orysata’ from rice in Pichia pastoris ppt

... incomplete process-ing of the signal peptide was also reported [16,17]. The uncleaved part of the a-mating sequence at the N-ter-minus as well as the histidine tag at the C-terminus of the recombinant ... strain X-33 with the addition of asignal sequence for secretion of the recombinant pro-tein into the medium. Approximately 12 mg of the recombinant lectin was puriﬁed from the medium of a1 L culture ... constrained the amino acid residuesforming the carbohydrate-binding site. The program tur-bofrodo was used to draw the Ramachandran plots [45]and perform the superimposition of the models....

Báo cáo khoa học: Functional analysis of the aglycone-binding site of the maize b-glucosidase Zm-p60.1 pot

... introducingfeatures of the consensus motif into the Zm-p60.1scaffold, an analysis of structural and catalytic prop-erties of the mutants, and simulations of the sub-strate–enzyme interactions of the wild-type ... F200Kand P2. Furthermore, the F193A and W373K muta-tions do not result in any change in the thermostability of the enzyme (Table S2), and thermal unfolding of the wild-type and both the F193A and ... anal-ysis of plant b-glucosidases to obtain insights into the evolution of the molecular sites involved in the twomodes of aglycone binding. The amino acid sequence of Zm-p60.1 was com-pared with the...

Báo cáo khoa học: Biochemical characterization of the major sorghum grain peroxidase pptx

... properties of SPC4. The absorption spectrum of puriﬁed SPC4 was recorded in 50 mMsodium acetate pH 5. The inset shows the spectrum of the extract-ed heme. (B) MALDI-TOF-MS analysis of SPC4 heme.Characterization ... andalso with the calculated mass based on the primarystructure (Table 2). The mass of the sugar moiety inHRP C is therefore 9782 Da, corresponding to 22.4%(w ⁄ w). HPAEC analysis of the HRP C ... absorption coefﬁcient of SPC4 at 403 nmwas determined to be approximately 104 mm)1Æcm)1.Figure 4(B) shows the mass spectral analysis of the extracted heme cofactor of SPC4. The mass of 616 Dacorresponds...

Báo cáo khoa học: Mutational analysis of the C-domain in nonribosomal peptide synthesis pot

... selectivity of the A-domains [9]simultaneously determines the primary sequence of the product. Subsequently the a ctivated amino acid i s tetheredto the terminal thiol moiety of a 4¢-phosphopantetheinyl(Ppant) ... furtherefforts were made to track down the actual target of the inhibitors.DISCUSSION The purpose of t his work was to identify the key residuesin the C-domain a nd their potential role in the ... d by the plot of Q19A. The second group (C154A, C154S, Y166F), represented by the plot of Y166F, is only impaired in DKP f ormation, implied by the slowdecresae of the acid stable label. The...

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