Báo cáo khoa học: Liver receptor homolog-1 localization in the nuclear body is regulated by sumoylation and cAMP signaling in rat granulosa cells docx

... ª 2008 The Authors Journal compilation ª 2008 FEBS Liver receptor homolog-1 localization in the nuclear body is regulated by sumoylation and cAMP signaling in rat granulosa cells Feng-Ming Yang, ... 2008) doi:10.1111/j.1742-4658.2008.06785.x Liver receptor homolog-1 (LRH-1; NR5A2) is an orphan member of the nuclear receptor superfamily, ma...

Ngày tải lên: 23/03/2014, 06:20

... depends on the concentration of the reaction product and the dissociation constant K i,B for inhibitor binding. The rate of hexose-based sucrose synthesis was described by the mass action rate law: r A!B ðtÞ¼k ... per hour. Data points were spline-interpolated to obtain time- continuous information on net photosynthesis during the whole period of cold exposure. The rate of r...

Ngày tải lên: 14/02/2014, 22:20

... Y 6 in brain. (B) Actin in brain. (C) Y 6 in peripheral tissues. (D) Actin in peripheral tissues. The negative control sample included water in- stead of cDNA. The brain regions are named in accordance ... Y 6 gene is contained within one exon and encodes a protein of 374 amino acids displaying the characteristics of other NPY family receptors (Fig. 1), including two wel...

Ngày tải lên: 19/02/2014, 07:20

... blocking was controlled by performing the labelling procedure in the absence of the second primary antibody. The sections were embedded in methylcellulose and examined in a Philips CM100 transmis- sion ... P i containing 5% BSA and 0.1% gelatine. Antibodies and gold conjugates were diluted in NaCl ⁄ P i containing 0.1% BSA-C (Aurion, Wageningen, the Netherlands). All...

Ngày tải lên: 07/03/2014, 11:20

... examine whether the DNA binding activity of dHAND could be affected by Akt phosphorylation. The in uence of phos- phorylation by Akt on the DNA binding activity was examined by gel shift analysis. ... cAMP- dependent protein kinase, protein kinase C and casein kinase II [36–42]. The activation of cAMP- dependent protein kinase or protein kinase C can inhibit muscle differe...

Ngày tải lên: 07/03/2014, 16:20

... to the b-globin gene promoter through the interaction with other DNA binding proteins involved in repressing the adult glo- bin gene. One possible candidate is BP1, a homeo- domain-containing ... to the b-globin gene promoter in K562 cells. In the present study, we ana- lyzed the interaction between TFII-I and HDAC3 in both MEL and K562 cells and show that this...

Ngày tải lên: 23/03/2014, 07:20

... self-association and to measure distances within proteins and other macromolecules [35]. In order to understand the folding and unfolding of peptides, both in the presence and the absence of PG lipids, ... may be due to internal quenching by the Lys next to the Trp [33]. Acrylamide quenching studies A prerequisite for any understanding of the interaction of the pep...

Ngày tải lên: 23/03/2014, 07:20

... significantly downregulated in imp a5 ) ⁄ ) mice, by  20%. These findings indicate that importin a5 is prominently involved in gene regulation by ER and its cofactors. On the other hand, the protein levels in ... upregulated in the brain, suggesting that the counter-regulation of another im- portin a isoform may compensate for the lack of a single isoform in vivo i...

Ngày tải lên: 28/03/2014, 23:20

... collected and then DNA-binding activity of NFjB p50 and p65 was assessed. There were no sig- nificant changes exhibited in the protein–DNA com- plexes induced by LPS in the presence of LXR agonists ... coactivator-1 (SRC-1) and activating signaling cointegrator-2 [34–39]. Another possible mechanism is that a direct protein– protein interaction between LXR and NFjBor betwe...

Ngày tải lên: 19/02/2014, 17:20

... Japan Introduction The epidermal growth factor receptor (EGFR) is com- posed of an extracellular ligand-binding domain, a transmembrane domain and an intracellular tyrosine kinase domain. The binding ... of the cell-cycle by phos- phorylating the retinoblastoma (RB) protein. Activa- tion of cyclin–CDK complexes is counterbalanced by CDK inhibitors, including those of the...

Ngày tải lên: 16/02/2014, 09:20