Báo cáo khoa học: Proteolysis of the tumour suppressor hDlg in response to osmotic stress is mediated by caspases and independent of phosphorylation pot

... E-cadherin or other protein(s) in the hDlg complex under stressful conditions, and the cortical cytoskeleton is therefore disrupted and hDlg released into the cytoplasm. The lack of effect of an hDlg ... regulation of early apoptotic events. Discussion Our aim was to gain a better understanding of the role of hDlg phosphorylation in the adaptation...

Ngày tải lên: 23/03/2014, 06:20

... reaction is independent of the presence of contaminating proteins and so is almost certainly autocatalytic. The insensitivity of the position o f cleavage to the amino- acid sequence a round the c ... rate of autoproteolysis of protein B, in response to environ- mental factors or the metabolic state of the cell. It is interesting to note that the...

Ngày tải lên: 31/03/2014, 09:20

... membrane and into the cytoplasm. This is initiated by cell surface binding of ETA to the a 2 MG ⁄ LRP1 receptor [4], which is followed by internalization of the toxin–receptor complex to the endosomal ... hand, and the mitochondrial membrane on the other hand. However, the potential role (if any) of ADP-ribosylation of EF-2 in the mitochondrial apopto...

Ngày tải lên: 18/02/2014, 04:20

... addition of ISP and Qcr10p). On the other hand, in the transition from the 500 kDa band to the 670 kDa band, a structural rearrangement of the bc 1 complex may occur due to the binding of ISP and ... for the insertion of ISP into an immature bc 1 complex and that the association of the Qcr9p subunit with the bc 1 complex precedes the binding of IS...

Ngày tải lên: 18/02/2014, 08:20

... the basic feature discriminating the 4F2hc proteins from both rBATs and GH13 enzymes is the lack of domain B protruding out of the TIM barrel in the place of loop 3 connecting the b3-strand to ... proteins. In all other groups, this domain is more or less distorted, culminating in complete loss in 4F2hc proteins. The presence of full GH13 domain B in hc...

Ngày tải lên: 18/02/2014, 13:20

... with the PRAD domain of ColQ indicates that the WAT domain of the C. intestinalis AChE is capable of organizing the sub- units into a tetramer through interaction with the PRAD domain of ColQ. The ... from C. intestinalis expressed in vitro and native enzyme expressed in vivo indicates the enzyme is AChE To determine the nature of the cholinesterase act...

Ngày tải lên: 18/02/2014, 17:20

... counterion. In contrast to the stabilizing effects of salts according to the Hofmeister series, the chloride salt is less destabilizing than the acetate salt. As discussed above, the inactiva- tion by ... of PLDa2 by calcium ions is due to the binding of cal- cium ions to the enzyme, and not (or not only) to bet- ter structuring of the substrate a...

Ngày tải lên: 19/02/2014, 02:20

... after 48 h of incubation in the two cell lines (Fig. 2A). Likewise, hypoxia decreased the levels of HO-1 and HO-2 proteins by more than 60% and 30%, respectively, in HeLa cells, and by 90% and 20%, ... of HO-1 and HO-2 mRNA in YN-1 cells To explore the mechanism of the hypoxia -mediated reduction of HO-1 and HO-2 protein levels, we ana- lyzed the e...

Ngày tải lên: 19/02/2014, 06:20

... The GLD1 pro- tein was tagged with a histidine tag at the N-terminal end by adding the coding sequence for six histidines to the end of the open reading frame, and then expressed in S. cerevisiae. ... glycerol and NADP in the oxidizing direction and activities with DHA or d-glyceraldehyde and NADPH in the redu- cing direction. According to the Internation...

Ngày tải lên: 19/02/2014, 06:20

... 20–564), and the model is composed of residues 25–540. The overall structure of the model of IPU and the mutated residues in this study are shown in Fig. 3. To elucidate the mechanism of the substrate ... 372, and 373 are the potential catalytic r esidues of I PU. Also, Trp425 of Dex49A, which is the corresponding residue of Trp402 of IPU, is...

Ngày tải lên: 19/02/2014, 16:20