Báo cáo khoa học: Lack of stabilized microtubules as a result of the absence of major maps in CAD cells does not preclude neurite formation pot

... characterized cytoskeleton and neurite formation in Cath .a- differen- tiated (CAD) cells, adding new information regarding this particular subject. CAD originated as a subclone of the cathecolamin- ergic ... trichostatin A (TSA) resulted in the appearance of a significant amount of acetylated tubulin (Fig. 1C, 3 and 6 h; Fig. 1D), indi- cating that both acetylase...

Ngày tải lên: 23/03/2014, 05:22

... FEBS General aspects of fatty acid oxidation Beta-oxidation is the preferred way of oxidizing FAs. In principle, each FA can be b-oxidized, including straight- and branched-chain FAs, as well as mono- and ... long-chain acyl-CoA dehydrogenase deficiency ACADVL VLCAD 17p11 201475 Medium-chain acyl-CoA dehydrogenase deficiency ACADM MCAD 1p31 201450 Short-chain acyl-CoA dehydrogenase...

Ngày tải lên: 22/03/2014, 16:21

... this strain and the ability of a cell harboring an individual library member to form a colony on minimal agar media lacking added phenylalanine and tyrosine reports on the chorismate mutase activity ... dehydrogenase protein com- plexes were replaced by genes encoding monofunctional versions of the dehydratase and the dehydrogenase. The growth of this strain on minimal med...

Ngày tải lên: 19/02/2014, 12:20

... Predictive information redundancy can be used as a measure of the redundancy between the predictive information of a feature type and that of a feature type combination. Predictive Information Summation ... predictive information gain of taking F x as a variant model on top of a baseline model employing F 1 ,F 2 , ,F i as feature type combination, is defined...

Ngày tải lên: 20/02/2014, 18:20

... N-terminal binding site [20]. It has to be noted,that using several lots of commercially available ATP–Sepharose the C-terminal binding was not always detected, especially when the assay was conducted under ... resins interact differently with the C-terminal nucleotide binding domain of Hsp90. Binding of Hsp90 to the commercially available and ‘lab-made’ ATP- Sepharose resins w...

Ngày tải lên: 08/03/2014, 02:21

... treated as a first-order reaction. The kinetic trace can be described mathematically by the equation DA t ¼ DA eq (1–e –kt )whereDA t is the increase in absorbance at time t, DA eq is the increase ... mutant is basically the same as that of the wild-type protein. However, the intro- duction of a hydroxyl group in the heme pocket, and the increased van der Waal...

Ngày tải lên: 08/03/2014, 10:20

... these kinases or, in the case of MEK inhibitors (such as AZD6244), inhibit one of the key signalling pathways they control; in all cases inhibition of the oncogenic kinase results in the loss of downstream ... problem clinically, and 40% of patients who relapse on imatinib therapy have point mutations in the BCR–ABL kinase domain, including the T315I gatekeeper...

Ngày tải lên: 16/03/2014, 00:20

... FEBS executing the terminal stage of a myriad of signaling cascades that initiate at the cell surface and reach their climax in the nucleus [8–10]. Upon activation by mitogen-activated protein kinas- es, ... change in thermal power as a function of each injection was automatically recorded using origin software and the raw data were further processed to yield binding i...

Ngày tải lên: 22/03/2014, 16:20

... binding, 5% of the input solution was kept aside separately and was used as a control for comparison. The proteins were analyzed in an 11% SDS ⁄ PAGE and by western blotting using anti-his Ig. As ... through the interactions of the DnaK–DnaJ–GrpE machinery and by HflB-mediated degradation. Interestingly, these latter proteins that promote the degradation of r 32 are the...

Ngày tải lên: 23/03/2014, 10:20

... p38 pathway acts predominately in the AGE-HSA and LPS amplified in ammatory response and, thus, may serve as a main therapeutic target for AGE-related in ammatory diseases. Furthermore, blockade of ... by activating mitogen-activated protein kinases and nuclear factor-jB in endothelial cells, thus amplifying the in ammatory response and resulting in tissue damage. Abbreviat...

Ngày tải lên: 30/03/2014, 01:20