Báo cáo khoa học: Lack of stabilized microtubules as a result of the absence of major maps in CAD cells does not preclude neurite formation pot

... characterizedcytoskeleton and neurite formation in Cath .a- differen-tiated (CAD) cells, adding new information regardingthis particular subject. CAD originated as a subclone of the cathecolamin-ergic ... trichostatin A (TSA)resulted in the appearance of a signiﬁcant amount of acetylated tubulin (Fig. 1C, 3 and 6 h; Fig. 1D), indi-cating that both acetylase and deacetylase were present in CAD cells. ... Glu-tubulin in CAD cells was not a result of a lack of (or inhibition of) the putative detyrosinating enzyme (tubulin carboxypepti-dase) because a signiﬁcant increase of Glu-tubulin wasobserved in differentiated...

Báo cáo khoa học: Fatty acid omega-oxidation as a rescue pathway for fatty acid oxidation disorders in humans pot

... FEBSGeneral aspects of fatty acid oxidationBeta-oxidation is the preferred way of oxidizing FAs. In principle, each FA can be b-oxidized, includingstraight- and branched-chain FAs, as well as mono-and ... long-chain acyl-CoA dehydrogenase deﬁciency ACADVL VLCAD 17p11 201475Medium-chain acyl-CoA dehydrogenase deﬁciency ACADM MCAD 1p31 201450Short-chain acyl-CoA dehydrogenase deﬁciency ACADS SCAD ... membrane bymeans of the carnitine cycle, which involves carnitinepalmitoyltransferase I, mitochondrial carnitine acylcar-nitine translocase and carnitine palmitoyltransferase II[5–7]. In case...

Tài liệu Báo cáo khoa học: Deciphering enzymes Genetic selection as a probe of structure and mechanism docx

... this strainand the ability of a cell harboring an individual library member to form a colony on minimal agar media lacking added phenylalanine and tyrosinereports on the chorismate mutase activity ... dehydrogenase protein com-plexes were replaced by genes encoding monofunctionalversions of the dehydratase and the dehydrogenase. The growth of this strain on minimal media lacking phenyl-alanine and ... a rationally designed DNA polymerase was used for the intro-duction in vivo of mutations in the TEM-1 b-lactamase geneat a desired frequency [51]. The cells containing thisengineered DNA polymerase...

Tài liệu Báo cáo khoa học: "An Information-Theory-Based Feature Type Analysis for the Modelling of Statistical Parsing" docx

... Predictive information redundancy can beused as a measure of the redundancy between the predictive information of a feature type andthat of a feature type combination. Predictive Information Summation ... predictive informationgain of taking Fx as a variant model on top of a baseline model employing F1,F2, ,Fi as featuretype combination, is defined as the differencebetween the conditional entropy ... evaluation modelfor statistical syntactic parsingGiven a sentence, the task of statistical syntacticparsing is to assign a probability to eachcandidate parsing tree that conforms to the grammar...

Báo cáo khoa học: by nucleotide afﬁnity cleavage: a distinct nucleotide speciﬁcity of the C-terminal ATP-binding site potx

... N-terminal binding site[20]. It has to be noted,that using several lots of commerciallyavailable ATP–Sepharose the C-terminal binding was not always detected, especially when the assay was conductedunder ... resins interactdiﬀerently with the C-terminal nucleotide binding domain of Hsp90.Binding of Hsp90 to the commercially available and ‘lab-made’ ATP-Sepharose resins was analyzed as described in ... (A) A nity cleavage assay. Hsp90 was a nity-cleaved in the presence of various nucleotides at a concentra-tion of 1 mM. Nucleotide binding was determined in the absence (N-terminal) or in the...

Báo cáo khoa học: X-ray crystallography, CD and kinetic studies revealed the essence of the abnormal behaviors of the cytochrome b5 Phe35ﬁTyr mutant pdf

... treated as a ﬁrst-order reaction. The kinetic tracecan be described mathematically by the equationDAt¼ DAeq(1–e–kt)whereDAtis the increase in absorbanceat time t, DAeqis the increase ... mutant is basically the same as that of the wild-type protein. However, the intro-duction of a hydroxyl group in the heme pocket, and the increased van der Waals’ and electrostatic interactionsbetween ... reﬁnement and was taken as the test dataset, and the agreement between the calculated and observedstructure factors of the test data set was monitoredthroughout the course of the reﬁnement. The graphicssoftwareTURBO-FRODO[27]...

Báo cáo khoa học: Apoptosis and autophagy: BIM as a mediator of tumour cell death in response to oncogene-targeted therapeutics pptx

... these kinases or, in the case of MEK inhibitors (such as AZD6244), inhibit one of the key signalling pathways they control; in all casesinhibition of the oncogenic kinase results in the loss of downstream ... problem clinically, and 40% of patients who relapse on imatinib therapy have pointmutations in the BCR–ABL kinase domain, including the T315I gatekeeper mutation that impairs imatinibbinding [58,59]. ... prosurvival extracellular signal-regulated kinase 1 ⁄ 2 (ERK1 ⁄ 2) and protein kinase B(PKB) pathways that act downstream of oncogenicprotein kinases [10,11]. It is increasingly apparent thatthese...

Báo cáo khoa học: Energetic coupling along an allosteric communication channel drives the binding of Jun-Fos heterodimeric transcription factor to DNA docx

... FEBSexecuting the terminal stage of a myriad of signalingcascades that initiate at the cell surface and reach theirclimax in the nucleus [8–10].Upon activation by mitogen-activated protein kinas-es, ... change in thermal power as a function of each injection was automatically recorded usingorigin software and the raw data were further processed toyield binding isotherms of heat release per injection ... domains containing Trx-tag at the N-terminus and His-tag at the C-terminus. Addition-ally, titration of a protein construct containing thioredoxinwith a C-terminal His-tag (Trx-His) in the calorimetric...

Báo cáo khoa học: Direct CIII–HﬂB interaction is responsible for the inhibition of the HﬂB (FtsH)-mediated proteolysis of Escherichia coli r32 by kCIII docx

... binding, 5% of the inputsolution was kept aside separately and was used as a controlfor comparison. The proteins were analyzed in an 11%SDS ⁄ PAGE and by western blotting using anti-his Ig. As ... through the interactions of the DnaK–DnaJ–GrpE machinery and by HﬂB-mediateddegradation. Interestingly, these latter proteins thatpromote the degradation of r32are themselves pro-duced as a result ... overnight at 22 °C. The protein mixture wasthen passed through a 1 mL benzamidine column (Amer-sham) for removal of thrombin. Finally, the native proteinwas separated from the mixture by treatment...

Báo cáo khoa học: Advanced glycation end products and lipopolysaccharide synergistically stimulate proinﬂammatory cytokine⁄chemokine production in endothelial cells via activation of both mitogen-activated protein kinases and nuclear factor-jB pdf

... p38pathway acts predominately in the AGE-HSA andLPS ampliﬁed in ammatory response and, thus, mayserve as a main therapeutic target for AGE-related in ammatory diseases. Furthermore, blockade of ... by activating mitogen-activatedprotein kinases and nuclear factor-jB in endothelial cells, thus amplifying the in ammatory response and resulting in tissue damage.AbbreviationsAGE, advanced ... impli-cating that the blockade of these signal pathways, in particular the p38 pathway, may provide a novelapproach for the treatment of AGE-related diseases,such as diabetes, complicated with bacterial...

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