Báo cáo khoa học: Substrate specificity and excision kinetics of natural polymorphic variants and phosphomimetic mutants of human 8-oxoguanine-DNA glycosylase pot
Tài liệu Báo cáo khoa học: Substrate specificity and inhibition of brassinin hydrolases, detoxifying enzymes from the plant pathogens Leptosphaeria maculans and Alternaria brassicicola ppt
... 8. Chemical structures of compounds tested as substrates of BHLmL2 and BHAb and potential products, references for synthesis,
and HPLC retention times using methods A and B described in the Experimental ... determined from the slope ()E
a
⁄ R) of Arrhenius
plots of log V (nmol of product per minute) versus
1 ⁄ T.
Substrate specificity
The substrate specificity of BHs...
Báo cáo khoa học: Substrate specificity and transport mode of the proton-dependent amino acid transporter mPAT2 potx
... Apparent K
m
and V
max
of various amino acids as a function of
the membrane potential in PAT2 expressing oocytes. Membrane
potential dependency of the apparent K
m
(A) and V
max
(B) values of
b-alanine ... also osmolytes such as sarcosine and
betaine, and the
D
-enantiomers of serine and alanine. The
apparent affinities of PAT1 substrates are mainly in the
range of 2–15...
Báo cáo khoa học: Substrate specificity and excision kinetics of natural polymorphic variants and phosphomimetic mutants of human 8-oxoguanine-DNA glycosylase pot
... Journal compilation ª 2009 FEBS
Substrate specificity and excision kinetics of natural
polymorphic variants and phosphomimetic mutants of
human 8-oxoguanine-DNA glycosylase
Viktoriya S. Sidorenko
1
, ... DNA,
to investigate the kinetics and substrate specificity of several known OGG1
polymorphic variants and phosphomimetic Ser fi Glu mutants. Am...
Báo cáo khoa học: Substrate specificity of the human UDP-glucuronosyltransferase UGT2B4 and UGT2B7 Identification of a critical aromatic amino acid residue at position 33 doc
... overlapping substrate specificity of these enzymes
remains to be elucidated.
Several studies have highlighted the role of the N-ter-
minal domain of UGTs in substrate specificity, and
many lines of evidence ... change the
specificity of this isoform.
The aim of this study was to identify amino acid res-
idues that are involved in substrate specificity of
UGTs 2B4 and...
Tài liệu Báo cáo khoa học: Substrate specificity of the pseudouridine synthase RluD in Escherichia coli doc
... in the loop of helix 69 of 23S rRNA variants; (b) substitution of
one U by C has no effect on the conversion of others (i.e. formation of
pseudouridines at positions 1911, 1915, and 1917 are ... RluD
has low substrate specificity, converting one out of 20
uridine residues in 23S rRNA and one out of eight uri-
dine residues in 16S rRNA to pseudouridine [9].
We have analyze...
Báo cáo khoa học: Substrate specificity of vaccinia virus thymidylate kinase docx
... ª 2005 FEBS
Phosphorylation of natural substrates and
analogues
Figure 3 shows the activity of vaccinia virus TMP kin-
ase as a function of natural substrates dTMP and ATP.
dTMP phosphorylation ... conserved in the
human and vaccinia enzymes.
A
BC
Fig. 1. Sequence and model for the structure of vaccinia virus TMP kinase and the human enzyme. (A) Alignment of the am...
Báo cáo khoa học: The specificity of alcohol dehydrogenase with cis-retinoids Activity with 11-cis-retinol and localization in retina docx
... an estimate of the effect of thecis-
bond position on the substrate specificity of human ADH4.
The 7-cis -and9 -cis-retinol and retinal isomers were the most
active substrates, in terms of k
cat
/K
m
,forADH4,followedby
11-cis-retinol ... coordinates of human ADH4 [35] were
used to simulate its interaction with all-trans,9-cis and 11-cis
isomers of retinol and retinal...
Báo cáo khoa học: Site specificity of yeast histone acetyltransferase B complex in vivo pot
... of
strains W303-1a (wild-type; +) and RS1263 (hat14; )) to a final
concentration of 200 m
M and, at the indicated time points, equal
amounts of cells were collected, and used for preparation of
WCEs. ... acetylation of any site on soluble
histone H4 and histone H3 except for Lys12 and Lys5
of histone H4.
Involvement of different components of the yeast
HAT-B complex in...
Báo cáo khoa học: Aglycone specificity of Escherichia coli a-xylosidase investigated by transxylosylation ppt
... Acceptor
specificity and regioselectivity were investigated using various sugars as
acceptor substrates and a-xylosyl fluoride as the donor substrate. Compari-
son of the rate of formation of the ... showed a
correlation between C-1¢ of the xylosyl moiety and H-6
of the fructosyl moiety, and the other showed a corre-
lation between C-1¢ of the xylosyl moiety and H-3 of...
Báo cáo Y học: Substrate specificity of human kallikrein 2 (hK2) as determined by phage display technology docx
... percentage of hydrolysis, the specificity
constant and the site of cleavage (11).
Ó FEBS 2002 Substrate specificity of kallikrein hK2 (Eur. J. Biochem. 269) 2749
nitrilotriacetic acid beads; the substrate ... inhibitor-1 and the Arg–Gln
motif is cleaved in IGF-BP-2. Using each of the 44
pentapeptides substrate sequences,
FASTA
and
BLAST
searches were done to look for new...
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