Báo cáo khoa học: Dissecting the role of the N-terminal metal-binding domains in activating the yeast copper ATPase in vivo pptx

... which appear to be involved in the final resorption of the parental macronucleus during PND in T. thermophila, and suggested the involvement of mitochondria in this process [19]. In mammalian apop- tosis, ... becomes acidic in the final step of PND [22,23]. DePsipher staining of the macronucleus appeared initially during the stage of autophagosome formation, and...

Ngày tải lên: 20/02/2014, 03:20

... Italy Introduction Towards the end of the 1970s, in the beginning of pre-mRNA splicing research [1,2], defining exons and introns was essentially based on observing the final composition of the mature ... Gunther V & Roesler J (2006) A splice- supporting intronic mutation in the last bp position of a cryptic exon within intron 6 of the CYBB gene induces its incor...

Ngày tải lên: 06/03/2014, 09:22

... leaves of barley and other species, and in whole plants: first, they induce novel abundant proteins designated jasmo- nat-induced proteins (JIPs); second, they repress the synthesis of photosynthetic ... 4666–4681 ª 2009 The Authors Journal compilation ª 2009 FEBS 4671 prodomains from caspases. Interestingly, the mycotox- in and sphingosine analogue fumonisin-B1 is a power- ful i...

Ngày tải lên: 16/03/2014, 02:20

... C-terminal suppressor of cytokine signalling (SOCS) box. SOCS3 and the related pro- tein, cytokine-inducible SH2-containing protein, are unique among the SOCS family of proteins in containing a region of mostly ... powerful tool in determining domain architecture. In this study we show that the N-terminal portion of the KIR of murine SOCS3 is unstructured, but the...

Ngày tải lên: 16/03/2014, 14:20

... other l-amino acids, including l-aspartate, l-glutamine, l-alanine, l-arginine, l-cysteine, l-proline, l-phenylalanine, l-lysine, l-tryptophan, l-isoleucine, l-tyrosine, l-histi- dine, l-leucine, ... standard conditions [32] following the instructions of the manufacturer. Cloning and sequencing of the alcohol dehydrogenase encoding gene The identification of the gene encoding a...

Ngày tải lên: 16/03/2014, 14:20

... distribution shows the prefer- ence of syntactic role of antecedents of a verb. Second, in the application phase, the syntactic role of an antecedent is decided using CPs and the syntactic role distribution. ... from the corpus. Then, the nominal words in the SRPs are substituted with their correspond- ing concept codes at level 4 of the Kadokawa thesa...

Ngày tải lên: 17/03/2014, 07:20

... demonstrates the results of the purification of the recombinant proteins. The occurrence of dominant bands of the recombinant proteins provides evidence of a high degree of purification obtained by ... could be involved in determining the unusual product profile of the pre- paration. The specific activity of the recombinant protein was rather low, being in t...

Ngày tải lên: 23/03/2014, 09:20

... an important role in the catalytic pro- cess of mIGPS and may be related to the inhibition mechanism of ATB107. To investigate the role of these residues in the inhib- itory effect of ATB107, we ... the autodock approach. The docking dummy center was arranged in the middle of the barrel composed of C-termini of b-sheets. The radius of the docking...

Ngày tải lên: 07/03/2014, 03:20

... N-terminus of the ATPase. To further study the role of the Ccc2 N-terminus in the overall function of the ATPase in vivo, we engi- neered DMBDCcc2 (Fig. 1E), a protein in which the N-terminus ... purified N-terminus and catalytic loop of the Wilson ATPase have shown that these two domains interact in the absence of copper and that their interactio...

Ngày tải lên: 23/03/2014, 05:22

... unsure of the mechanism of DNA-induced stabilization of the protein, and whe- ther it is related to the normal RNA-binding function of coat protein, as RNA binding had no effect. The increased ... against all the forms of denatura- tion we tested. This is consistent with the observation of increased stability of the single-chain version of the wild-type dimer [...

Ngày tải lên: 30/03/2014, 11:20