Báo cáo khoa học: Structural insight into the evolutionary and pharmacologic homology of glutamate carboxypeptidases II and III ppt

... both GCPIII and GCPII, including the ‘glutarate sensor’ and the Pro-rich region [27]. Both GCPII and GCPIII display an induced-fit movement of the ‘glutarate sensor’ upon binding of a glutamate (or glutamate- like) ... therapeutics and ligands, as well as in the investigation of the diverse biological functions of GCPIII and GCPII. Experimental procedures rhGCPI...

Ngày tải lên: 23/03/2014, 05:22

... erva- tamin-A, the P3 moiety of both the molecules of the asymmetric unit runs along the extended backbone of residues 65–64 and is partly exposed to the solvent (Fig. 2B). On the other hand, the P3 ... at the bottom of the subsite is conserved in ervatamins. Compared to a Ser in the equivalent position of papain, the role of Leu here is to restrict the s...

Ngày tải lên: 18/02/2014, 16:20

... portions to the substrate solution during 1 h at 25 °C. Formation of the product was accompanied by the development of a yellow and persistent color in the solution. Then, the pH of the mixture ... excess of nitrite (from 40 l M to 1.6 m M ). The compound II reduction to iron (III) followed a first-order behavior. In order to decrease the noise in the readings, t...

Ngày tải lên: 16/03/2014, 16:20

... ion in the P-loop and Glu172 to the 3¢-OH of the substrate. In the dTTP complex, the phosphates are bound by the LID argi- nines and the 3¢-OH is bound to Glu172. In these cases, Glu171 in the LID ... comparing the phosphorylation of the natural substrates of the double mutant with the single mutant, it seems that the mutation N45D in the double mutant co...

Ngày tải lên: 30/03/2014, 20:20

... 7. The arrows labeled (b) and (c) point to the areas of interaction between the transmembrane helix of subunit 8 and helices G and H1 of cytochrome b and between the N-terminus of subunit 8 and ... panels A and B and the relative amounts of each of the subunits determined by densitometry scanning of the stained Western blots of the q° and CKL57...

Ngày tải lên: 19/02/2014, 12:20

... alignment of the amino acid sequences of RVV-X HC with those of other represen- tative P -III enzymes. It shows highest similarity (82%) to VLFXA HC, and lower similarity to other P -III proteases, ... sequenced. Of these, 10 clones were found to encode the LC2 and LC1 sub- units. Others were found to encode other variants of the C-lectin-like venom proteins. The amino...

Ngày tải lên: 07/03/2014, 06:20

... the measurement of the rate of passive diffusion of the dye through the plasma membrane. This cannot be done by the simple measurement of the increase of the fluorescence signal (F cyto ) of the ... show that the efficiencies of the P-gp-mediated efflux of Rh I, Rh II, TMR, Rh 6G, Rh B, RhI ,II hydro- lysed are similar to each other and to the efficiency...

Ngày tải lên: 08/03/2014, 08:20

... initiation of cDNA synthesis. Because most of these mutants were able to cleave single- and double-stranded DNA substrates (Fig. 4), the impairment of mobility may reflect the requirement of these ... [2,20–25]. The L. lactis Ll.ltrB intron and the yeast aI1 and aI2 introns, which are the best studied mobile introns and serve as a paradigm for group II intron mobili...

Ngày tải lên: 15/03/2014, 09:20

... help translate the cellular and biochemical insights into a better understanding of disease mechanism. In this minireview, we discuss the contributions of animal models to our growing understanding of the ... biology of cavernous malformations, including the elucidation of the cellular context of CCM protein actions and the in vivo confirmation of abnormal endot...

Ngày tải lên: 15/03/2014, 10:20

... VioC•L-arginine•tar- trate•Fe (II) . The b-strands B, G, D, I and C build the major side of the jelly roll fold, and the minor side is built by the b-strands F, E and H. The flexible lid region is shown in blue, the bound ... contains three helices (a1–a3) and one b-strand (A) parallel to the first b-strand, B, of the jelly roll core. The linkage of the fourt...

Ngày tải lên: 16/03/2014, 02:20