... comparison with the one obtained at pH 6.0. No preferential loss of signal in the region of any of the aromatic residues was observed, suggesting a global loss of the tertiary structure of ovorubin. Enzymatic ... regard to pH In order to evaluate the influence of pH on ovorubin struc- ture, solutions of 0.24 mgÆmL )1 of the protein at different pH values (...
Ngày tải lên: 07/03/2014, 06:20
... in the IM becoming freely permeable to protons, leading to the uncoupling of oxidative phosphorylation. MPT is caused by the opening of the nonselective, highly conductive PTP in the mitochon- drial ... from the generation of an electro- chemical gradient across the IM forms the basis of the IM w m . During cell death, MP often increases, allow- ing for the relea...
Ngày tải lên: 16/02/2014, 09:20
Tài liệu Báo cáo khoa học: Arabidopsis thaliana BTB⁄ POZ-MATH proteins interact with members of the ERF⁄AP2 transcription factor family ppt
... interaction requires a complete MATH domain and the N-terminal region of RAP2.4 The use of a truncated version of BPM1 in the Y2H screens demonstrated that the BTB domain is not involved in the assembly ... RAP2.13. To further confirm the interaction of RAP2.4 and RAP2.13 with BPM proteins, we cloned the corre- sponding full-length cDNAs for both genes, generated GST fusi...
Ngày tải lên: 18/02/2014, 06:20
Tài liệu Báo cáo khoa học: High affinity copper binding by stefin B (cystatin B) and its role in the inhibition of amyloid fibrillation docx
... obtained from injections of the metal into a solution of the protein. Typically an initial injection of 2 lL copper chelate solution was followed by a further 29 injections of 4 lL of Cu(II) into the ... oligomers are the cause of the initial insult rather than the insoluble fibrous material. Environmental factors, among them metal ions, are believed to contribute...
Ngày tải lên: 19/02/2014, 06:20
Tài liệu Báo cáo khóa học: Determination by electrospray mass spectrometry and 1H-NMR spectroscopy of primary structures of variously fucosylated neutral oligosaccharides based on the iso-lacto-N-octaose core doc
... by cone voltage fragmentation [14]. The fragment ions of m/z 729 and m/z 544 are both from the monofucosylated D 2b-5 ion m/z 1037 and not from the difucosylated D 2b-5 ion m/z 1183. Thus the ... in the 6-branch, and believed to be from a contaminant (see below), having the same molecular mass with one Fuc in each of the 3- and 6-branches. Comparison of the product...
Ngày tải lên: 19/02/2014, 12:20
Báo cáo khoa học: Protective effect of dietary curcumin and capsaicin on induced oxidation of low-density lipoprotein, iron-induced hepatotoxicity and carrageenan-induced inflammation in experimental rats ppt
... alteration in endothelial function, are considered among the early events in the pathogenesis of atherosclerosis [43]. The alteration of oxidant ⁄ antioxidant balance may affect the susceptibility of ... influence the activity of serum alkaline phospha- tase and acid phosphatase in iron(II)-injected rats, only the combination of spice principles significantly coun- tered...
Ngày tải lên: 08/03/2014, 08:20
Báo cáo khoa học: Dual effect of echinomycin on hypoxia-inducible factor-1 activity under normoxic and hypoxic conditions docx
... enhance Sp1 activity in these conditions. This effect was no longer observed at higher concentrations. The protein level of Sp1 was checked in the different conditions: the results showed that ... result of the loss of the molecule during the extraction procedure: indeed, only the nuclear proteins are recovered and the DNA is dis- carded. By contrast, when echinomycin...
Ngày tải lên: 16/03/2014, 05:20
Báo cáo khoa học: Protective effect of active oxygen scavengers on protein degradation and photochemical function in photosystem I submembrane fractions during light stress pdf
... scavengers used that only the generation of 1 O 2 is responsible for the photooxidation effects. The role of active oxygen scavengers in the protection of the structure and function of the photo- systems ... The main site of PSI photo- inhibition under aerobic conditions was then shown to be the inactivation of iron–sulfur centers [11], whereas anaerobic photoinhib...
Ngày tải lên: 16/03/2014, 18:20
Báo cáo khoa học: 2-Oxo acid dehydrogenase complexes in redox regulation Role of the lipoate residues and thioredoxin pot
... assess the net effects of redox perturbations that reflect integrated metabolic responses, dissecting the mechanisms of these overall responses requires investigation of the separate components of the ... pyruvate dehydrogenase phosphorylation depends on the state of the complex-bound dihydrolipoate. Thus, the redox regulation of the eukaryotic pyruvate dehydrogen...
Ngày tải lên: 17/03/2014, 09:20
Báo cáo khoa học: Cell death-inducing DFF45-like effector, a lipid droplet-associated protein, might be involved in the differentiation of human adipocytes pdf
... 23 (b) and 33 (c) of gestation (scale bar = 25 lm). The expression of CIDEC increased along with the differentiation of adipose tissue, and the highest level of expression of CIDEC was detected in ... between the 14th and 16th weeks of gestation in humans, and that the second tri- mester of gestation is the critical period in adipogenesis. After the 23rd week of...
Ngày tải lên: 23/03/2014, 03:20