Báo cáo khoa học: Identification of structural determinants for inhibition strength and specificity of wheat xylanase inhibitors TAXI-IA and TAXI-IIA doc

... was used for G1 N-T, the region that was used for G1 pC-T and region that was used for G1 dC-T are marked in different shades of gray. S385, S401 and T407 are marked in black (bold and underlined). ... catalytic subunit of PKA (PKA-cs) and [ 32 P]ATP[cP]. Autoradiograms of subsequent SDS gels revealed GIRK1 migrating in two bands, indicating a glycosylated and a nonglyco...

Ngày tải lên: 07/03/2014, 00:20

... characterization of the precise structural and functional properties of oxidized HSA. Here, we characterize both the structural and functional differences between reduced and oxidized HSA. Using LC-ESI- TOFMS ... frag- ment of HSA. N, nonoxidized HSA; H, highly oxidized HSA. (B) Densitometric analysis of intensities of undigested HSA. Changes in intensities of HSA bands...

Ngày tải lên: 16/03/2014, 13:20

... Journal compilation ª 2009 FEBS 3927 Identification of structural determinants for inhibition strength and specificity of wheat xylanase inhibitors TAXI-IA and TAXI-IIA Annick Pollet 1 , Stefaan ... area of Pro294 rTAXI-IIA , compared with that of Leu292 TAXI-IA (6.8% and 10.1%, respectively). Structural basis for the inhibition of BSX by TAXI-IA...

Ngày tải lên: 23/03/2014, 05:22

... Munson, R.S. Jr (1999) Identification of a novel sialyltransferase gene from Haemophilus ducreyi and construction of mutants deficient in the production of sialic acid containing glycoforms of the lipooligo- saccharides. ... further information on the nature of the sialylated glycoforms. Comparison of the total ion electropherogram in negative ion mode (Fig. 3A) and selective...

Ngày tải lên: 23/03/2014, 21:21

... was performed with RGG3 (lanes 2 and 4) and 32 P-labeled Htelo (lanes 1 and 2) or rHtelo (lanes 3 and 4). (B) EMSA was performed with RGG3 (lanes 2 and 4) and 32 P-labeled rHtelo (lanes 1 and 2) ... 1. Affinity of EWS for binding to G-quadruplex DNA. (A) EMSA was performed with EWS (lanes 2 and 4) and 32 P-labeled ETS-1 (lanes 3 and 4) or ssDNA L (lanes 1 and 2). (B)...

Ngày tải lên: 15/02/2014, 01:20

... internal control for RT-PCR. PCR condi- tions were as follows: one cycle of 94 °C for 3 min, 32 cycles of 94 °C for 30 s, 54 °C for 30 s and 72 °C for 30 s, followed by one cycle of 72 °C for 5 min. ... expression of T-bet has been seen within a wide variety of tissues and cells in the Ginbuna crucian carp [27] and, in mammals, in the lung tissue of mouse and sp...

Ngày tải lên: 16/02/2014, 09:20

... and structural analysis of OSs in LOSs of lpxX and lpxL mutants The OS portion of each LOS was obtained after mild acid hydrolysis and analyzed for its glycosyl composi- tions and by MALDI-TOF ... chloroform (1 : 1 v ⁄ v). A new volume of chloroform was used for each of the three extractions. Finally, the organic phase was re-extracted with de-ionized water to remove t...

Ngày tải lên: 18/02/2014, 14:20

... properties of SmSmad1 and SmSmad1B as described above. However, in the presence of SmSmad4 and the active mutant form of TbRI, both SmSmad2 and SmSmad4 interacted with GCN5, indicating the formation of ... Oliveira 2 and Philip T. LoVerde 1,2 1 Department of Microbiology and Immunology, School of Medicine and Biomedical Sciences, State University of New York, NY, USA...

Ngày tải lên: 18/02/2014, 16:20

... reactions were performed in triplicates. Amplification conditions were 40 cycles with the following profile: 95 °C for 30 s, 60 °C for 30 s, and 72 °C for 1 min. For each kind of tissue, standard curves were ... overexpression approach of RpCAbr and RpCAtr, we cannot know the functional effect of changes of some key amino-acids. Origin and number of nonvertebrate CAs Alt...

Ngày tải lên: 18/02/2014, 16:20

... HSPs (HSP70 and HSP23) were greatly and rapidly upregu- lated in gills but slightly less and later in mantle. One inducible and two constitutive isoforms of HSP70 are synthesized in the gills and mantle ... metabolism and translational processing. Total RNA was extracted from gills and mantle of 10 oysters exposed to 13 and 25 °C for 0, 3, 7, 10, 14, 17 and 24 days. A...

Ngày tải lên: 18/02/2014, 16:20