Báo cáo khoa học: Polysaccharide binding sites in hyaluronate lyase – crystal structures of native phage–encoded hyaluronate lyase and its complexes with ascorbic acid and lactose docx

... 339 2–3 402 ª 2009 The Authors Journal compilation ª 2009 FEBS Polysaccharide binding sites in hyaluronate lyase – crystal structures of native phage–encoded hyaluronate lyase and its complexes with ... respectively. The crystal structures of native HylP2 and its two complexes with ascorbic acid and lactose have been determined....

Ngày tải lên: 23/03/2014, 04:21

... of the protein). Discussion Examination of the previously determined crystal structures of native and inhibited forms of Exg from C. albicans revealed that this enzyme recruits the same set of ... atlas of surface topography of proteins with structural and topographi- cal mapping of functionally annotated residues. Nucleic Acids Res 34, W116–W118. W. M. Patrick et...

Ngày tải lên: 15/03/2014, 23:20

... DNA- binding properties of murine Six3, in vitro binding site selection demonstrated high-affinity binding to sequences containing the TAAT core motif, and clus- ters of these tetranucleotide sites ... 60 amino acid HD, which is a DNA -binding domain, has a conserved global fold consisting of three a-helices and a flexible N-terminal arm that becomes more ordered upon DNA...

Ngày tải lên: 29/03/2014, 08:20

... peptide fragments obtained after 3 or 30 min of incubation in the presence of ATP and after 3 h of incubation in the absence of ATP w ere also analyze d in the same way. The yields of the fragments ... y amino -acid analyses. The results are shown in Fig. 4. Twenty-seven cleavage sites were identi®ed with the sample incubated for 30 min in the presence of ATP. Dur...

Ngày tải lên: 21/02/2014, 03:20

... 2011) doi:10.1111/j.1742-4658.2011.08043.x Starch -binding domains are noncatalytic carbohydrate -binding modules that mediate binding to granular starch. The starch -binding domains from the carbohydrate -binding module family ... b-cyclodextrin injection, and the bottom panel depicts the binding isotherm; open circles represent the integrated binding heat of the data in the...

Ngày tải lên: 06/03/2014, 00:21

... RPE65c was incubated with liposomes con- taining atRE (A), liposomes containing atRE in the presence of 1m M bipyridine (B) and liposomes containing atRE, in the presence of 1 m M bipyridine and 6 ... magnification images of the boxed area in (B4) showing RPE65c staining (B5), GS staining (B6), merged RPE65c and GS staining (B7), and DAPI staining (B8), respectively. Whit...

Ngày tải lên: 14/02/2014, 14:20

... identified no other inner membrane proteins in our vesicle pre- paration, we suggest that Gut2 is an outer membrane protein with its N-terminal FAD -binding domain facing the intermembrane space. ... reliable indicator of whether a protein is integral in the outer membrane [17,2 0–2 2]. As a distinct means to separate integral and peri- pheral membrane proteins we undertook clou...

Ngày tải lên: 19/02/2014, 07:20

... Creutz- feldt–Jakob and Parkinson’s diseases [ 5–8 ]. Increased levels of sHsps, in particular aB-crystallin and Hsp27, are observed in the brains of sufferers of these diseases [9,10]. Hsp25 is the murine ... (2002) Role of the C-terminal extensions of a-crystallins: swap- ping the C-terminal extension of aA-crystallin to aB- crystallin results in enhanced chaperone a...

Ngày tải lên: 07/03/2014, 04:20

... MINIREVIEW Alternative binding proteins: Anticalins – harnessing the structural plasticity of the lipocalin ligand pocket to engineer novel binding activities Arne Skerra Lehrstuhl ... bilin -binding protein (BBP) of Pieris brassi- cae [19] was employed to engineer an artificial binding site for ligands such as fluorescein and digoxigenin, as well as other small molecules and...

Ngày tải lên: 23/03/2014, 07:20

... binding are shown in orange and those involved in nucleotide binding are shown in red. Residues involved in the binding to the PLP moiety are shown in blue, whereas those involved in protein–protein ... PDZ interactions with the C-terminal amino acid triplet of serine were crucial for binding, but instead, the interaction was established with its N-terminal 66 r...

Ngày tải lên: 30/03/2014, 04:20