Báo cáo khoa học: Insulin is a kinetic but not a thermodynamic inhibitor of amylin aggregation pot

... significant inhibitory and promotional effects on amylin aggregation. In the SEC analysis (Fig. 6), the peak of insulin supernatant almost disappeared after 48 h of incubation, suggesting that amylin insulin complexes ... various caseins during their fibrillation. Alto- gether, these results indicate that insulin is a kinetic but not a thermodynamic inhibitor of...

Ngày tải lên: 23/03/2014, 04:21

... GGATCCTTTAAGCG GCAAGCAA), AtGPX2 (CATATGGCGGATGAATC TCCAA ⁄ GGATCCTCCTCCTCCTGGTGAT), AtGPX5 (CATATGGGTGCTTCATCATCAT ⁄ GG ATCCCTGTG CGTGTTCACAA) and AtGPX6 (CATATGGC AGC AGAGAAGTCTG ⁄ GGATCCAGTTATCCAGATTGAA) without ... GGATCCAGTTATCCAGATTGAA) without transit peptides or Trx h2 (CATATGGGA GGAGCTTTATC ⁄ GGATCCGCGTTAACAATGCTCA) and Trx h3 (CATATGGAAGAGAAGCCGCA ⁄ GGATCC AAATCAAGCAGCAGC) proteins...

Ngày tải lên: 16/03/2014, 12:20

... Dictionary and Dynamically Estimated Domains of Unknown Words Chikara Hashimoto Graduate School of Science and Engineering Yamagata University Yonezawa-shi, Yamagata, 992-8510, Japan ch@yz.yamagata-u.ac.jp Sadao ... Japan ch@yz.yamagata-u.ac.jp Sadao Kurohashi Graduate School of Informatics Kyoto University Sakyo-ku, Kyoto, 606-8501, Japan kuro@i.kyoto-u.ac.jp Abstract This paper presen...

Ngày tải lên: 31/03/2014, 00:20

... structure. This paper presents a way of utilizing statistical decision trees to systematically raise the memory capacity of Markov models and effectively to make Markov models be able to accommodate various ... performances. For ex- ample, we often find that in some cases a certain lexical context can improve the performance of an HMM-based POS tagger, but incorporating such ad- di...

Ngày tải lên: 31/03/2014, 03:20

... in a mutant that lacks NirF; this too is not trivial as the DnirF strain does not accumulate readily detectable amounts of an intermediate of d 1 synthesis. Experimental procedures DNA manipulations DNA ... 25 2.2 2 1.8 1.4 1.2 1.6 1 0.8 0.6 0.4 0.2 0 A 600 A 600 A 600 Time (h) Fig. 5. His41 is essential for Paracoc- cus pantotrophus NirF, but Asp129 is dis- pensable....

Ngày tải lên: 15/02/2014, 01:20

... that bacitracin should not be regarded as a specific inhibitor of PDI. Results Bacitracin does not inhibit the catalysis of disulfide bond formation and isomerization by PDI PDI is a catalyst of ... point. Table 1. Analysis of the aggregation rate during rhodanese refold- ing at pH 7.2. The rate of aggregation relative to the negative con- trol in the absence of baci...

Ngày tải lên: 16/02/2014, 14:20

... human intestinal epithelial cells (Caco-2). Cell 60, 429–437. 5 Misumi Y, Hayashi Y, Arakawa F & Ikehara Y (1992) Molecular cloning and sequence analysis of human dipeptidyl peptidase IV, a ... Allison JP, Chesner JE, Leger MJ, Ridge LL & Walborg EF Jr (1983) Characterization of a family of glycoproteins associated with the bile canalicu- lar membrane of normal hepatocytes...

Ngày tải lên: 19/02/2014, 07:20

... peroxisomal aspartate aminotransfer- ase Aat2p in HPLC fraction 7 at a molecular mass of approximately 45 kDa (Fig. 1A) [15]. The predicted molecular mass of Lpx1p is 44 kDa. It carries a peroxisomal ... Woolford CA, Noble JA, Garman JD, Tam MF, Innis MA & Jones EW (1993) Phenotypic analysis of protein- ase A mutants. Implications for autoactivation and the maturation pathwa...

Ngày tải lên: 07/03/2014, 05:20

... The absorbance was determined at 492 nm with plate reader (Sunrise, Tecan, Gr ¨ odig, Austria). Data analysis Data were analyzed by Student’s t-test. A value of P < 0.05 was considered statistical ... reaction volume with rTaq DNA polymerase or LA Taq TM DNA polymerase with GC buffer (TaKaRa). Human NANOGP8 mRNA was amplified by RT-PCR using total RNAs extracted from urinary bladder can...

Ngày tải lên: 07/03/2014, 12:20

... plasmid pUG27 [13] using the primers disSUT2fwd 5¢-TGACGCTCACCAAGCTATTGGTTT GTTTGGATCAATCGTCAGATATGAAGGCATAG GCCACTAGTGGATCTG-3¢ and disSUT2rev 5¢-TAT TAATATTCCTATATTTTACATAGGAGGAAATTA CATGCATGAAACCTACAGCTGAAGCTTCGTAC GC-3¢, ... S.pombe his5 + construct amplified from plasmid pUG27 as described above using the primers disRAS1fwd 5¢-TTCACGATTGAACAGGTAAACAAAATTTTCC CTTTTTAGAACGACATGCAGCTGAAGCTTC...

Ngày tải lên: 07/03/2014, 15:20