Báo cáo khoa học: Proteomic characterization of lipid raft proteins in amyotrophic lateral sclerosis mouse spinal cord pot

... effective enrichment of lipid raft proteins using the centrifugation protocol. Proteomic analysis of mouse spinal cord lipid rafts To identify proteins in lipid rafts, purified lipid raft fractions ... same mouse; and (b) the protein was consistently identified in the independent Fig. 1. Evaluation of the isolation of lipid raft proteins. Lipid raft...

Ngày tải lên: 23/03/2014, 04:21

... generated by desolvation of the phosphate group. Nevertheless, although the binding enthalpy of the inhibitors is negative, the binding enthalpy of 6PG is Table 1. Binding parameters of substrate and ... aPy- ADP (a nonoxidizing analogue of NADP) are reported in Table 2. A binding isotherm and the fitted data for the binding of aPyADP to the enzyme are shown in Fig. 1. The bind...

Ngày tải lên: 07/03/2014, 05:20

... mode of binding and interference with enzyme catalysis. As the inactivation of any enzyme involved in a metabolic chain will render the whole chain inoperat- ive, the inactivation of any of the ... of the six proteins involved in pyrimidine de novo synthesis should result in the same profound effect on the pyrimidine nuc- leotide pools in C. albicans. However, in mammali...

Ngày tải lên: 07/03/2014, 12:20

... the amino acids are shown to the rightofeachline.ThesequenceoftheN-ter- minal 25 amino acid residues (IEP-1) deter- mined in the prior study [21] is underlined. The amino acid sequence (10 amino ... respectively, instead of the calyx cells. Further, the expression of IEP proteins is not synchronized with that of CkPDV capsid proteins; IEP proteins were observed approximately one...

Ngày tải lên: 31/03/2014, 15:20

... domain containing inositol 5-phosphatase, includes a series of protein interacting domains and has the ability to dephosphorylate phosphatidylinositol 3,4,5-trisphosphate [PtdIns(3,4,5)P 3 ] in ... localization of Src homology 2-containing inositol 5¢-phosphatase 2 via Shc association is required for the negative regula- tion of insulin signaling in Rat1 fibroblasts overexpress- ing...

Ngày tải lên: 16/03/2014, 19:20

... the interpretation is confirmed that the driving force for an optimal packing of the acyl chains is their linkage and distribution to the two glucosamines, causing the observed high inclination independent ... 10 min at 400 g and stored at )20 °C until deter- mination of cytokine content (see above). Determination of endotoxin activity by the chromogenic Limulus test Endotoxin activity...

Ngày tải lên: 23/03/2014, 13:20

... Regardless of dopamine treatment, overexpres- sion of a-synuclein significantly affected the levels of four proteins [stathmin 1, glutathione S-transferase (GST)p, Ran 1 binding protein and C1q binding ... suggested to be linked to early stages of PD pathogenesis [26]. Interestingly, in our model, dopamine induces an increase of the actin bundles regulator fascin 1 [27] and disco...

Ngày tải lên: 15/02/2014, 01:20

... proteins are a large family of RNA-binding proteins found in all eukaryotes. These proteins reduce the expression of mRNA targets by binding in 3¢ UTR regulatory elements, thus controlling translation ... alignment of the APUM proteins with the PUF domains most similar to Drosophila PUF domain. (A) APUM proteins of group I. (B) APUM proteins of group II. C. W. Franci...

Ngày tải lên: 18/02/2014, 06:20

... catalysis, ligand binding, and the role of storage proteins, the cupins constitute one of the most diverse superfamilies of proteins known. They have been described from all three domains of life [1,2], ... concentration of the 16 kDa protein subunit) increased linearly with increasing fractional saturation of the metal site in Bxe_A2876, the k cat determined from the molar c...

Ngày tải lên: 18/02/2014, 06:20

... stock of 0.5 nm radiolabeled 5¢ exon mimic in H10Mg buffer at 44 °C was added. The mixture was incubated at 44 °C for at least 90 min. To maintain the integrity of the bound species during gel ... site can play a role in determining the binding regis- ter of reaction substrates. Biochemistry 44, 1067–1077. P. P. Dotson II et al. Kinetics of the trans excision-splicing reaction FEB...

Ngày tải lên: 18/02/2014, 18:20