Báo cáo khoa học: Conformational stability and multistate unfolding of poly(A)-specific ribonuclease docx

... article. Multistate unfolding of PARN G J. He et al. 2860 FEBS Journal 276 (2009) 2849–2860 ª 2009 The Authors Journal compilation ª 2009 FEBS Conformational stability and multistate unfolding of poly(A)-specific ... Allosteric regulation of human poly(A)-specific ribonuclease by cap and potassium ions. Biochem Bio- phys Res Commun 379, 341–345. 24 Jaenicke R (1999)...

Ngày tải lên: 23/03/2014, 04:21

... dissociation and denaturation of Hc. The sigmoidal plot of A 340 ⁄ A 280 vs. salt concentration can be interpreted in terms of a conformational transition between a native oligomeric state and an unfolded state, ... identified by the strict conservation of the chemical features of amino acids listed in Table 1, C or I, and, to some extent, also I* and C*. The lower stabilit...

Ngày tải lên: 23/03/2014, 13:20

... methods used, and then the testing results of the MLRs and the MDR. In this paper, we report the results of the four types of anaphora, namely NAME-ORG, QZPRO-ORG, DNP-ORG, and ZPRO-ORG, ... of the MDR. MLR-6 shows the effect of not training on anaphoric chains. It results in poorer performance than the MLR-1, 2, 3, 4, and 5 configurations and the MDR. One of the...

Ngày tải lên: 23/03/2014, 20:21

... analyses of the kinetic and thermodynamic origin of protein thermo- stability and hyperthermostbility, however, have often been hampered by unfolding irreversibility of such pro- teins in vitro ... thermodynamic stability (DG U ), and kinetic folding ⁄ unfolding parameters (k f and k u ) for hyperthermostable proteins. If not otherwise stated, T M and DG U refer to pH...

Ngày tải lên: 07/03/2014, 05:20

... nuclear magnetic resonance study of the cyclic AMP receptor protein (CRP): assignments of the NH protons of histidine and tryptophan residues and the effect of binding of cAMP to CRP. Biochem J 107, 304–309. 14 ... k on and k off for the interaction of ICAP, lac and gal pro- moters observed in this work result from the extent of the DNA bending. This suggestion is in con...

Ngày tải lên: 07/03/2014, 16:20

... Ulrih and T. Lanis ˇ nik Riz ˇ ner Conformational stability of 17b-HSDcl FEBS Journal 273 (2006) 3927–3937 ª 2006 The Authors Journal compilation ª 2006 FEBS 3937 Conformational stability of 17b-hydroxysteroid dehydrogenase ... Ulrih 1 and Tea Lanis ˇ nik Riz ˇ ner 2 1 Department of Food Science and Technology, University of Ljubljana, Slovenia 2 Institute of Biochemis...

Ngày tải lên: 16/03/2014, 13:20

... Alessandra Corazza 1 , Paolo Viglino 1 , Luc Moens 3 , Gennaro Esposito 1 and Angelo Fontana 2 1 Department of Biomedical Sciences and Technologies and MATI Centre of Excellence, University of ... cleavage sites of apoMb undergo hydrolysis, as a consequence of the stability of the helical fold of the protein [11]. An important determinant responsible for the conforma- ti...

Ngày tải lên: 30/03/2014, 01:20

... Sevcik J, Wilson KS, Dauter Z, Hartley RW et al. (1998) Conformational stability and thermody- namics of folding of ribonucleases Sa, Sa2 and Sa3. J Mol Biol 279, 271–286. 34 Pace CN & Thomson ... rest of the molecule, in agreement with the larger rmsd of the solution bundle in these regions [14]. Residues in the loop between strands b4 and b5 (Thr149, Asn151 and Lys15...

Ngày tải lên: 30/03/2014, 04:20

... Fluorescence titration of the FMN domain with the hMS AD. Fig. S2. Comparison of the electrostatic potentials of the surface of the CPR FMN domain and of a model of the FMN domain of human MSR. Fig. ... mammalian difla- vin reductases and also subdomains of MSR; (b) the extent of reductive remethylation of hMS catalysed by the different redox states of MSR; and (c)...

Ngày tải lên: 18/02/2014, 08:20

... dis- tribution of the energy between the overall tumbling and the internal mobility, and is indicative of the degree of internal restraint of each 15 N– 1 H vector motion. In Fig. 4, most of the points ... inter- pretation [4] and provides support for our previous proposal. The link between the nearly absolute invari- ance of Asn51 and Arg53 and the conformational dyna...

Ngày tải lên: 18/02/2014, 16:20