Báo cáo khoa học: Allosteric and binding properties of Asp1–Glu382 truncated recombinant human serum albumin – an optical and NMR spectroscopic investigation pot
Báo cáo khoa học: Allosteric and binding properties of Asp1–Glu382 truncated recombinant human serum albumin – an optical and NMR spectroscopic investigation pot
... mgÆmL )1 in serum of healthy human adults) makes it an important determinant of the pharmacokinetic behavior of many drugs. HSA also accounts for most of the antioxidant capacity of human serum. Further- more, ... truncated albumin G. Fanali et al. 2250 FEBS Journal 276 (2009) 224 1–2 250 ª 2009 The Authors Journal compilation ª 2009 FEBS Allosteric and binding...
Ngày tải lên: 23/03/2014, 04:21
Tài liệu Báo cáo khoa học: Understanding the binding properties of an unusual metal-binding protein ) a study of bacterial frataxin pdf
... A ˚ and 2.6 A ˚ ) and Glu19 (2.7 A ˚ ), and two water molecules (1.7 A ˚ and 1.9 A ˚ ) (Fig. 4E). In M5, the side chains of Asp76 (1.9 A ˚ ) and Asp27 (2.4 A ˚ ) of a symmetry-related molecule and ... without significant chemical shift variations. Among these were residues 5, 1 0–1 8, 20, 21, 39, 5 3–5 5, 57, 60, 61, 7 0–7 3 and 75, and the indole side chains of Trp...
Ngày tải lên: 18/02/2014, 16:20
Báo cáo khoa học: The starch-binding capacity of the noncatalytic SBD2 region and the interaction between the N- and C-terminal domains are involved in the modulation of the activity of starch synthase III fromArabidopsis thaliana pdf
... 3D): they span residues 31 6–3 44 in the D2 domain [D(31 6–3 44)] and residues 49 5–5 35 in the D3 domain [D(49 5–5 35)]. Co-expression and purification of CD and SBD recombinant proteins Protein–protein ... detected with anti-GS (lane 2) or anti-D123 (lane 3) serum. (E) Pull-down assays for D1 (left panel), D2 (middle panel) and D3 (right panel). The first lane of each p...
Ngày tải lên: 22/03/2014, 21:20
Báo cáo khoa học: Acute intermittent porphyria – impact of mutations found in the hydroxymethylbilane synthase gene on biochemical and enzymatic protein properties pdf
... Christiansen L, Petersen NE, Horder M & Kristiansen K (2005) Characterization of two iso- alleles and three mutations in both isoforms of purified recombinant human porphobilinogen deaminase. Scand J ... 11q24.1-q24.2 of the long arm [7]. The HMBS gene is divided into 15 exons of 3 9–4 38 bp in length and 14 introns of 8 7–2 913 bp in length and spans approximately...
Ngày tải lên: 23/03/2014, 04:21
Tài liệu Báo cáo khoa học: Post-translational modification of the deubiquitinating enzyme otubain 1 modulates active RhoA levels and susceptibility to Yersinia invasion pptx
... the probe. OTUB1 and OTUB1–probe adduct were visualized by immunoblotting using HA antibodies and quantified for two experiments (black and grey bars). The intensities of the corresponding bands were ... immunoprecipitation of OTUB1. Eluted material was analysed by SDS ⁄ PAGE gel and Coomassie Blue staining, and the band corresponding to a modified OTUB1 (rectangle) was excised a...
Ngày tải lên: 16/02/2014, 15:20
Tài liệu Báo cáo khoa học: Insights into the structure of plant a-type phospholipase D Susanne Stumpe, Stephan Konig and Renate Ulbrich-Hofmann ¨ ppt
... Bittova L, Sumandea M & Cho W (1999) A structure– function study of the C2 domain of cytosolic phospholi- pase A2. Identification of essential calcium ligands and hydrophobic membrane binding residues. ... maximum at 192 nm; and (b) a wide minimum between 208 and 220 nm. The a-helix and b-strand contents of the protein were calculated with the online programs dichroweb [2...
Ngày tải lên: 19/02/2014, 02:20
Tài liệu Báo cáo khoa học: DNA strand exchange activity of rice recombinase OsDmc1 monitored by fluorescence resonance energy transfer and the role of ATP hydrolysis pptx
... highly distance dependent between donor and acceptor dyes, will therefore unveil the sta- tus of physical union and separation of complementary strands during renaturation and strand exchange, respectively, ... reflected changes consistent with strand exchange reaction. Effect of homology dependence and ATP hydrolysis on strand exchange activity Strand exchange was monitored as a...
Ngày tải lên: 19/02/2014, 07:20
Báo cáo khoa học: The enzyme-binding region of human GM2-activator protein pdf
... the lipid- and the enzyme -binding of this cofactor, we combined a variety of functional and bio- physical analyses. Crystallographic analysis of the homodimeric iso- enzyme HexB [24,25] and subsequent ... showed 7–8 % of the WT activity, whereas the mutants D113K and E123K displayed almost a complete loss of activity. To assess the membrane activity and lipid -binding...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: A client-binding site of Cdc37 ppt
... with both anti-Myc and anti-FLAG polyclonal antibodies (WB: a-Myc and a-FLAG). Asterisks indicate nonspecific bands appearing in every lane. K. Terasawa and Y. Minami A client -binding site of Cdc37 FEBS ... similar experiment using the N- and C-terminal portions of Cdc37, namely FLAG–Cdc37( 1– 163) and FLAG–Cdc37(16 4–3 78), respectively, and found that FLAG–Cdc37(16 4–...
Ngày tải lên: 07/03/2014, 21:20
Báo cáo khoa học: On the aggregation properties of FMRP – a link with the FXTAS syndrome? pot
... disorder in humans. In a previous study, we described the biochemical and biophysical aggregation properties of constructs spanning the conserved region of FMRP and of two other human fragile ... for understanding granule formation and could have a link with the pathogenesis of FXTAS. Results FXR proteins present aggregation and amyloidogenic hot-spots To understand the ag...
Ngày tải lên: 14/03/2014, 23:20