Báo cáo khoa học: Probing the substrate specificities of matriptase, matriptase-2, hepsin and DESC1 with internally quenched fluorescent peptides potx

... subfamilies: matriptase, matrip- tase-2, hepsin and DESC1. We determined their pH optimum, their k cat , K m and k cat ⁄ K m values toward a number of internally quenched fluorescent (IQF) peptides and their ... picture of the relative activities of matriptase, matriptase-2, hepsin and DESC1 towards the fluorogenic peptides, 18 IQF peptides were incu-...

Ngày tải lên: 23/03/2014, 04:21

... they are only taken to indicate the presence of the specific holocytochrome under investigation if the features of the spectrum, in terms of wavelength maxima, and the position and intensity of ... pylori. The two systems can each be subdivided into proteins which contribute to the handling of heme and ligation of heme to the apocytochrome, and those involve...

Ngày tải lên: 06/03/2014, 09:22

... associated with tomato fruit aroma, and use substrate docking analysis to assess the basis for the substrate specificity. Results and Discussion Cloning and sequence analysis of SlUGT5 The SGN Unigene ... UGT72B1) (Fig. S3). In the Arabidopsis UGT72B1 structure, an interaction between Tyr315 and the main-chain atoms of Ser14 and Pro15 anchors this loop within the...

Ngày tải lên: 28/03/2014, 23:20

... carboxydipeptidase activities and have compared them with those of human recombinant cathepsin B and cathepsin L [21]. Therefore we also comparatively a nalyzed the carboxydipeptidase activit- ies of rCPB3 and r H84Y ... ivities of CPB3 and H 84Y were also observed u sing the peptide Abz-FRAK(Dnp)-OH and some of its ana- logues. The kinet ic parameters of hydrolysi...

Ngày tải lên: 23/03/2014, 13:20

... formed new major bands with either GDR or DDR in the presence of ADP or in the absence of nucleotides (Fig. 4A,B, lanes 3 and 4). In the presence of ATP, the new major band with DDR did not appear, ... (DR), and GDR (GR) are indicated with arrowheads to the right of the gels, and their complexes 1–4 to the left. Bands 1 and 2 correspond to 1 : 2 and 1 : 1...

Ngày tải lên: 16/03/2014, 05:20

... (brown sphere) and the catalytic facial triad of H204, H209 and D356. (D,E) Molecular sur- faces of the substrate channel leading to the binding pocket of the WT AtdA3 (D) and the mutant I248L (E). The substrate ... entry and exit of substrate and product molecules, explaining the A B DE C Fig. 3. (A) The homology model of the AtdA3, with the s...

Ngày tải lên: 19/02/2014, 02:20

... stabilize the aromatic and the hydrocarbon chain of the FA. The importance of the interaction between an aromatic tyrosine and the phenolic ring of the substrate is consistent with the biochemical ... side of the pyranose ring of a sugar monomer, aligns itself upon binding with the aromatic ring face of the amino acid to contribute to selectivity o...

Ngày tải lên: 30/03/2014, 20:20

... requires further structural insight is, on the one hand, the widespread use of Asp at the P7 position in many naturally occurring NADPH-dependent GDHs and, on the other hand, the failure of the E243D ... titrations showed that even with these mutations binding of NADPH was still too weak to allow determination of a K d value in the absence of the other substrate....

Ngày tải lên: 30/03/2014, 03:20

... to judge the information content of the translations, the translation quality, and the user-friendliness. The list of criteria for self evaluation consisted of technical, linguistic and ergonomic ... translated but comes with an incorrect determiner (wristband : die Handgelenkband instead of das Handge- lenkband). c (correct) The translation is correct. Out...

Ngày tải lên: 22/02/2014, 03:20

... picture of the enzyme and should be helpful in a comprehensive under- standing of the role of hKAT-I, especially in KYNA biosynthesis in the human brain. Experimental procedures Enzyme expression and ... shown). Incorporation of a-ketobutyrate or other a-keto acid concomitantly decreased and increased the 335 nm and 422 nm peak, respectively, and the relative dimen...

Ngày tải lên: 07/03/2014, 16:20