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Báo cáo khoa học: Mechanism of mild acid hydrolysis of galactan polysaccharides with highly ordered disaccharide repeats leading to a complete series of exclusively odd-numbered oligosaccharides doc

Báo cáo khoa học: Mechanism of mild acid hydrolysis of galactan polysaccharides with highly ordered disaccharide repeats leading to a complete series of exclusively odd-numbered oligosaccharides doc

Báo cáo khoa học: Mechanism of mild acid hydrolysis of galactan polysaccharides with highly ordered disaccharide repeats leading to a complete series of exclusively odd-numbered oligosaccharides doc

... 2137 Mechanism of mild acid hydrolysis of galactan polysaccharides with highly ordered disaccharide repeats leading to a complete series of exclusively odd-numbered oligosaccharides Bo Yang1, Guangli ... Kurosawa M, Sumi T, Xia X& Yamaguchi K (1998) A gas chromatographic methodfor the sugar analysis of 3,6-anhydrogalactose-contain-ing algal galactans. Anal Biochem 265, 42–48.28 Hama Y, Nakagawa ... positions.1RK2RK3RK4RK5RK6RK7RK8RK1K2K3K4K5K6K7K8K1RA2RA3RA4RA5RA6RA7RA8RA1I2I3I4I5I6I7I8I-S-S 1A 2A 3A 4A 5A 6A 7A 8A A B C D E Fig. 1. Gel filtration chromatography of j-carra -oligosaccharides and agaro -oligosaccharides and their alditols. (A) j-Carra- oligosaccharides resulting from mild acid hydrolysis. ...
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Báo cáo khoa học: "Minimized models and grammar-informed initialization for supertagging with highly ambiguous lexicons" pdf

... IntroductionCreating accurate part -of- speech (POS) taggersusing a tag dictionary and unlabeled data is aninteresting task with practical applications. Ithas been explored at length in the literature ... constrainlearning—prior to considering any particular lan-guage, grammar or data set. Baldridge (2008) usesthis observation to create grammar-informed tagtransitions for a bitag HMM supertagger ... given a data set, while the latter biases bitag transitionstoward those which are more likely based on a uni-versal grammar without reference to any data. Inthis paper, we show how these strategies...
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Tài liệu Báo cáo khoa học: Purification and structural characterization of a D-amino acid-containing conopeptide, conomarphin, from Conus marmoreus docx

Tài liệu Báo cáo khoa học: Purification and structural characterization of a D-amino acid-containing conopeptide, conomarphin, from Conus marmoreus docx

... 275–283.10 Kuwada M, Teramoto T, Kumagaye KY, Nakajima K,Watanabe T, Kawai T, Kawakami Y, Niidome T,Sawada K, Nishizawa Y et al. (1994) Omega-agatoxin-TK containing D-serine at position 46, but ... D-amino acid in peptide link-age by an enzyme from frog skin secretions. Proc NatlAcad Sci USA 102, 4235–4239.33 Shikata Y, Watanabe T, Teramoto T, Inoue A, Kawakami Y, Nishizawa Y, Katayama K & ... Kubota I, Takao T, Shimonishi Y,Yasuda-Kamatani Y, Minakata H, Nomoto K,Muneoka Y & Kobayashi M (1991) Fulicin, a novelneuropeptide containing a D-amino acid residueisolated from the ganglia...
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Tài liệu Báo cáo khoa học: Mechanism of dihydroneopterin aldolase NMR, equilibrium and transient kinetic studies of the Staphylococcus aureus and Escherichia coli enzymes docx

Tài liệu Báo cáo khoa học: Mechanism of dihydroneopterin aldolase NMR, equilibrium and transient kinetic studies of the Staphylococcus aureus and Escherichia coli enzymes docx

... S. aureus genomic DNA. ThepET17H vector was used for the production of a His-tagged SaDHNA. The primers for the PCR were 5¢-GGAATTCCATATG CAAGACA CAAT CTTTCTT AAAG-3¢(forward primer with a ... deprotonation and reprotonation of C2¢. The alter-native reaction can be initiated by deprotonation of C1¢ and protonation of N5 to form an enol intermedi-ate, which can turn into a keto intermediate ... the same reaction intermediate as that of the aldolase reaction (Fig. 1), whereas it has to beextracted by a base if the epimerase reaction followsthe mechanism of deprotonation and reprotonation...
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Tài liệu Báo cáo khoa học: Mechanism of protection of peroxidase activity by oscillatory dynamics docx

Tài liệu Báo cáo khoa học: Mechanism of protection of peroxidase activity by oscillatory dynamics docx

... decreasing pH. We were not able to measure the rate of inactivation by further decreases in pH,because other factors, such as increased autooxidation of NADH and other acid degradation of this ... experimental data, we increased theinfusion rate of NADH to obtain a higher rate of production of NAD+(Table 2, osc*). Comparing the two oscillatorystates (Table 2, osc and osc*) to the steady state ... in a decrease in the rate of enzyme inactivation. However, whenthe reaction is in a stationary state, the concentration of thearomatic cofactor does not seem to have any effect on therate of...
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Báo cáo khoa học: Energetic and metabolic transient response of Saccharomyces cerevisiae to benzoic acid docx

Báo cáo khoa học: Energetic and metabolic transient response of Saccharomyces cerevisiae to benzoic acid docx

... steady-statesis that the concentrations of the weak acids in thetricarboxylic acid cycle (pyruvate, citrate, a- ketogluta-rate, succinate, fumarate and malate) in the presence of benzoic acid are all ... yeasts such as Saccharomyces cerevi-siae and Zygosaccharomyces bailii, both of which areknown to be important food spoilage yeasts, are able to adapt to the presence of these weak acids with ... the plasma membrane H(+)-ATPase and optimalglycolytic flux are required for rapid adaptation andgrowth of Saccharomyces cerevisiae in the presence of the weak -acid preservative sorbic acid. Appl...
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Báo cáo khoa học: Two L-amino acid oxidase isoenzymes from Russell’s viper (Daboia russelli russelli) venom with different mechanisms of inhibition by substrate analogs pdf

Báo cáo khoa học: Two L-amino acid oxidase isoenzymes from Russell’s viper (Daboia russelli russelli) venom with different mechanisms of inhibition by substrate analogs pdf

... a ligand to enter andanchor at the respective functional sites of LAAO that may facilitate thedesign of suicidal inhibitors.AbbreviationsLAAO,L-amino acid oxidase; NAT, N-acetyltryptophan; ... half-reaction involving the conversion of FAD(flavin cofactor) to FADH2and concomitant oxidation of the amino acid to an imino acid. The imino acid intermediate of the oxidation pathway undergoesnonenzymatic ... Amersham Pharmacia Biotech (2001) Chromatofocusing with Polybuffer and PBE, pp. 15–24. Amersham Phar-macia Biotech AB, Uppsala.Inhibitor-binding sites of L-amino acid oxidase S. Mandal and D. Bhattacharyya2094...
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Báo cáo khoa học: Reconstruction ofde novopathway for synthesis of UDP-glucuronic acid and UDP-xylose from intrinsic UDP-glucose inSaccharomyces cerevisiae pptx

Báo cáo khoa học: Reconstruction ofde novopathway for synthesis of UDP-glucuronic acid and UDP-xylose from intrinsic UDP-glucose inSaccharomyces cerevisiae pptx

... TOY1 and TOY3 strains (leftpanel). The AtUXS3 enzyme was detectable as a 40-kDa band with a c-Myc antibody in the TOY2 and TOY3 strains (right panel). Lane1, Control W30 3a strain; lane 2, TOY1 ... identified in A. thaliana and they can be classifiedinto two types. AtUXS1 and AtUXS2 are type IImembrane proteins localized to the Golgi. AtUXS3lacks an N-terminal transmembrane region and is a soluble ... is a substrate of b1,3-glucan synthase and b1,6-glucan synthase in thesynthesis of cell wall b-glucan polysaccharides, is abun-dant in the cytoplasm [19–21]. Therefore, yeast appears to have...
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Báo cáo khoa học: Studies on structure–function relationships of indolepyruvate decarboxylase from Enterobacter cloacae, a key enzyme of the indole acetic acid pathway ppt

Báo cáo khoa học: Studies on structure–function relationships of indolepyruvate decarboxylase from Enterobacter cloacae, a key enzyme of the indole acetic acid pathway ppt

... molecular mass standards.Small angle X-ray solution scattering with synchrotronradiation. Data were collected on the X33 camera of theEuropean Molecular Biology Laboratory outstation atHasylab at ... detail, a coupled opticalassay was elaborated with alcohol dehydrogenase asauxiliary enzyme, catalysing the aldehyde–alcohol conver-sion similar to the assays established for pyruvate decarb-oxylase ... Fig. 5. A kcat of 3.81 ± 0.24 s)1andanS0.5-value of 0.7 mMwas calculated according to the rateequation for substrate activation [18].Cofactor binding experimentsCofactor binding was studied...
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Báo cáo khoa học: Mechanism of the reaction catalyzed by dehydroascorbate reductase from spinach chloroplasts doc

Báo cáo khoa học: Mechanism of the reaction catalyzed by dehydroascorbate reductase from spinach chloroplasts doc

... Takayama, Ikoma, Nara 630-0101, Japan.Fax: + 81 743 72 5569, Tel.: + 81 743 72 5560,E-mail: yokota@bs.aist-nara.ac.jpAbbreviations: APX, ascorbate peroxidase; AsA, ascorbate; DHA,dehydroascorbate; ... 5¢-AGCTTGTTGGGGGTGGTGAC-3¢;P2,5¢-AATCTGTCACCACCCCCAAC-3¢;P3,5¢-CCTTGACGGATATTTGGAGTG-3¢;P4,5¢-TGGCGATTCTCCATTTTGCCAAAGAGTG-3¢;andP5,5¢-TGGCGATTGTCCATTTTCCCAAAGAGTG-3¢.Mutatedbases are underlined. The ... (Kuma-moto, Japan). Other chemicals and reagents were of thehighest purity commercially available.Assay of the activity of DHARThe reaction rate of DHAR was determined by monitoringthe glutathione-dependent...
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