... strategy involved the insertion of several fragments of the 5¢-region of mce-1 into the mul-tiple cloning site of the vector pPD95.77 provided by A.Fire (Carnegie Institute, Baltimore, MD, USA). The ... of the domains occurs in several differentways, suggesting that the evolution of these proteinsprobably involved multiple gene duplication, genefusion and domain swapping events. Members of ... cleft, with the first strand of the N-terminal module antiparallel to the first strand of the C-terminal module. At the bot-tom of this U-shaped cavity is the metal binding site,where the divalent...