Báo cáo khoa học: Biochemical analysis of the human EVL domains in homologous recombination doc

... a deletion analysis with EVL, which revealed that the EVH2 domain is responsible for the recombination activity of EVL. We found that the EVH2 domain: (a) contains the ssDNA-binding and dsDNA-binding activities; ... [15–18]. These actin-binding activities are conserved in the EVH2 domain [19,20]. On the other hand, the EVH1 domain binds to proteins containing the con...

Ngày tải lên: 23/03/2014, 04:20

... co-exist. DNA-binding activity of the human DMC1-I37N variant The Ile37 residue of the DMC1 protein is located in the N-terminal domain. The corresponding N-terminal domain of the RAD51 protein was suggested ... performed in the presence of the DMC1 (6 lM) or DMC1-I37N (6 lM) protein in the same manner as described in (B), except for the presence of th...

Ngày tải lên: 23/03/2014, 06:20

... ordering in the protein during nucleotide binding. These structural changes were also observed dur- ing changes in the oligomerization state. We observed only a minor in u- ence of the C-terminal ... con- served in the primary structure of the GTPase domain of hGBP5, so the formation of GMP must be impaired in an as yet unknown manner. Sequence alignments of th...

Ngày tải lên: 15/03/2014, 10:20

... strategy involved the insertion of several fragments of the 5¢-region of mce-1 into the mul- tiple cloning site of the vector pPD95.77 provided by A. Fire (Carnegie Institute, Baltimore, MD, USA). The ... of the domains occurs in several different ways, suggesting that the evolution of these proteins probably involved multiple gene duplication, gene fusion and do...

Ngày tải lên: 23/03/2014, 13:20

... FEBS 4773 of Q A – in PSII centers that are affected by the nonsatu- rating concentration of inhibitor [7,8]. The E m of Q A is raised in the presence of DCMU in the Q B pocket, making it energetically ... to the reduction of the PQ pool. Results As reported in the literature, the I step of the O–J–I–P fluorescence transient cannot be clearly distinguished...

Ngày tải lên: 19/02/2014, 05:20

... C-terminal region, including the PAS-B domain of BMAL1, is required for the binding to DEC1, Fig. 4. Identification of the domain of D EC1 that interacts with BMAL 1. Interactions of various DEC1 ... region of DEC1, including the bHLH domain, interacted with the C-terminal r egion o f BMAL1 in a mammalian two-hybrid assay. Accordingly, a Fig. 5. Analysis of DEC1 mutan...

Ngày tải lên: 19/02/2014, 16:20

... structure of the N-terminal domain of the human homologue, solved at 2.2 A ˚ resolution [11]. The obtained model covers residues 254–568 of HpPex5p, corresponding to the C-terminal half of the protein ... concluded from the structural model of the PTS1-binding C-terminal domain of HpPex5p (Fig. 4). In this domain three of the seven Trp residues of HpPex5p a...

Ngày tải lên: 21/02/2014, 00:20

... flavin-binding domain of ferre- doxin : NADP + reductase [10]. The lysine residue is not conserved; instead, an arginine residue interacts with the FAD molecule of the enzyme. In the case of FMN-binding ... that calculated from the amino acid sequence, indicating that the native form of flavoredoxin is a dimer. Amino acid sequence analysis of flavoredoxin The N-termin...

Ngày tải lên: 07/03/2014, 02:20

... with GST–a4 in the presence of His–PP2Aca (data not shown). Similar results were obtained using the PP2Ac-binding domain of a4, a4D222 [24], instead of the full-length protein (Fig. 4B), which further ... derivative strains containing pACT2-PP2Aca and the indi- cated TIPRL mutant cDNAs fused to the lexA DNA-binding domain of pTL1 were plated on synthetic minimal medium lac...

Ngày tải lên: 07/03/2014, 05:20

... simi- larly docked into the saccharide-binding site of Calsepa (RCSB Protein Data Bank code 1OUW) [28]. Cartoons showing the docking of Man ⁄ MeMan in the mannose-bind- ing sites of the lectins were ... energy over the threshold value. The docking of MeMan into the carbohydrate-binding sites of Orysata and other JRLs was performed with the program insightii. The...

Ngày tải lên: 14/03/2014, 23:20