Báo cáo khoa học: Production of a recombinant mouse monoclonal antibody in transgenic silkworm cocoons pptx

... 5. Analysis of N-glycans in the recombinant mAb by lectin blot- ting. Recombinant mAb was subjected to lectin blotting with AAL and concanavalin A. The purified recombinant mAb (R) and standard human ... 10 4 Fig. 4. Antigen binding of recombinant mouse mAb. The binding of recombinant mouse mAb to human IgG was analyzed by ELISA. Recombinant mAb (closed triangles), hy...

Ngày tải lên: 23/03/2014, 04:20

... substantia nigra pars compacta and appearance of Lewy bodies consisting of aggregated protein, mainly a- synuclein, in Keywords amyloid; fibrillation; Parkinson’s disease; synuclein; thioflavin T Correspondence I. ... H, Okamura K, Bauer PO, Furukawa Y, Shimizu H, Kurosawa M, Machida Y, Miyazaki H, Mitsui K, Kuroiwa Y et al. (2008) RNA-binding protein TLS is a major nuclear aggregate-i...

Ngày tải lên: 14/02/2014, 19:20

... Ohno-Iwashita Y, Shimada Y, Waheed AA, Hayashi M, Inomata M, Nakamura M, Maruya M & Iwashita S (2004) Perfringolysin O, a cholesterol-binding cytolysin, as a probe for lipid rafts. Anaerobe ... 10, 125–134. 19 Waheed AA, Shimada Y, Heijinen HFG, Nakamura M, Inomata M, Hayashi M, Iwashita S, Slot JW & Ohno- Iwashita Y (2001) Selective binding of perfringolysin O derivative to cho...

Ngày tải lên: 20/02/2014, 03:20

... CLAP_2:AAGTCTGTCGTCAAAATGTTATGAACGTCTCTTGTCATAAAGAAAGAGAACCTCTCTTTTTAGTTTGGTTTAGATATTAAGGACAGATCCAAAATATTTG 1132 * CLAP_1:AGGACCTTTTATTAGACATTTCAAATATATAATAAACGTTATTTTA AAATTAGAAAAATTGAAAGACAAGCTAATGAAAGCTTATTGCCGATTGGAAAGT 1300 CLAP_2:AGGACCTTTTATTAGACATTTCAAATATATAATAAACGTTATTTTA AAATTAGAAAAATTGAAAGACAAGCTAATGAAAGCTTATTGCCGATTGGAAAGT ... CLAP_2:ATTAGTGCTATAGTTTGGGAAATATTTAGTCCTTGTTTTG...

Ngày tải lên: 07/03/2014, 16:20

... plot of AMP–AMP phosphotransferase activity (as percentage of total activity) in mixtures containing various amounts of AdK (0.1–4.0 nmol in a final volume of 0.25 mL) and a fixed amount of MK ... 0.3 AdK activity in the presence of A1 34974 0 AdK activity in the presence of Ap 5 A 5.46 ± 0.3 Table 3. MK, AdK, ADA and AMP–AMP phosphotransferase activi- ties in nor...

Ngày tải lên: 23/03/2014, 06:20

... 0.2 M GalNAc GalNAca1 fi 3Gal Sophora japonica (SJA) Galb1 fi 3GalNAc 0.2 M Gal Galb1 fi 3,4GlcNAc Wistaria floribunda (WFL) a/ bGalNAc 0.2 M GalNAc Helix pomatia (HPL) GalNAca1 fi 3GalNAc 0.2 M GalNAc GalNAca1 ... within 10 days. Quantification of alkaline phosphatase and aminopeptidase activities Specific alkaline phosphatase (ALP) and N-aminopeptidase (APN) enzymatic activities of BBMV prote...

Ngày tải lên: 23/03/2014, 13:20

... GACGACGACAAGATGGACACCAACTTCCCCATCTGCATCTTCTGCTG TAAATGCTGTAACAATTCCCAGTGTGGTATCTGTTGCAAAACATAA Mouse hepcidin reverse GAGGAGAAGCCCGGTTATGTTTTGCAACAGATACCACACTGGGAATT GTTACAGCATTTACAGCAGAAGATGCAGATGGGGAAGTTGGTGTCCA Human hepcidin ... forward GACGACGACAAGATGGACACCCACTTCCCGATCTGCATTTTCTGCTG CGGCTGCTGTCATCGATCAAAGTGTGGGATGTGCTGCAAGACGTAA Human hepcidin reverse GAGGAGAAGCCCGGTTACGTCTTGCAGCACATCC...

Ngày tải lên: 18/02/2014, 18:20

... 4753 Application of a fluorescent cobalamin analogue for analysis of the binding kinetics A study employing recombinant human transcobalamin and intrinsic factor Sergey N. Fedosov 1 , Charles ... Haber E & Olesen H (1971) Nature of vitamin B 12 binding. II. Steric orientation of vitamin B 12 on binding and number of combining sites of human intrin- sic factor and the tran...

Ngày tải lên: 19/02/2014, 05:20

... (2007) Surface-scanning mutational analy- sis of protein arginine methyltransferase 1: roles of specific amino acids in methyltransferase substrate spec- ificity, oligomerization, and coactivator function. ... Purandare AV, Chen Z, Huynh T, Pang S, Geng J, Vaccaro W, Poss MA, Oconnell J, Nowak K & Jayar- aman L (2008) Pyrazole inhibitors of coactivator associ- ated arginine methyltr...

Ngày tải lên: 06/03/2014, 11:20

... GGGGATCCGTCGACCTGCAGCGTACGAGGAAAGGAAATAGGC cyc1-y GTTTAAACGAGCTCGAATTCATCGATCCGTCAACGACAGTTG cyc1-z GCATCAGAAAGCATAGGC cyc1-m TGGGAATACGATAGAGTAG nb2 primer GTTTAAACGAGCTCGAATTC Coq7 in fission yeast R. ... GGGGATCCGTCGACCTGCAGCGTACGACATACTACTTCATTTG Spcoq3-y GTTTAAACGAGCTCGAATTCATCGATCCTAGCGTTACCGTTG Spcoq3-z GTATGCGATGTGGAATTTG Spcoq3-m GATGCCTTCCAATGAATTAC cyc1-w GAACCAATGAAATAAGGGCG cyc1...

Ngày tải lên: 18/02/2014, 14:20