Báo cáo khoa học: Identification of domains involved in the allosteric regulation of cytosolic Arabidopsis thaliana NADP-malic enzymes ppt

... concentrations. The d 2 -crystallin in lens was recruited during evolution from argininosuccinate lyase, an enzyme involved in arginine biosynthesis derived from the urea cycle. These two proteins shared ... stability for crystallins is essential in maintaining lens transparency [4]. Determining the mechanism of folding and assembly of these proteins is important for underst...

Ngày tải lên: 08/03/2014, 08:20

... chain is brefeldin A-insensitive and is inhibited by the proteasome inhibitor clasto-lactacystin b-lactone, resulting in the accumulation of ricin A chains. These stabilized ricin A chains are partly deglycosylated ... pivotal role of the UPR is to maintain ER home- ostasis. Therefore, the presence of mutated proteins that are unable to fold into their native conformation in...

Ngày tải lên: 16/03/2014, 11:20

... control. Functional domains in FPRL1 Y. Le et al. 774 FEBS Journal 272 (2005) 769–778 ª 2005 FEBS the involvement of multiple domains of FPR in recogni- tion of the agonist fMLF, including the first, second, and ... half of the first TM domain through the second intracellular loop of FPRL1 with the corresponding part of FPR (A). Chimera H contains the N-terminu...

Ngày tải lên: 16/03/2014, 18:20

... on the guanidini- um group of the Arg in the RGG domain in the C-terminal of EWS. Results and Discussion Several DNA-binding proteins that bind to G-quadru- plex DNA have been investigated in ... nucleolin [21– 27]. The RGG domain of FMRP, which is an RNA- binding protein involved in nerve cell differentiation, interacts with the G-quartet forming RNA [22–25]. In...

Ngày tải lên: 15/02/2014, 01:20

... all-alpha domain, STAT protein DNA-binding domain and SH2 domain of stat6, and the zinc-finger domain, leucine-zipper domain and fork-head domain of foxp3. In addition, for stat6 and foxp3, there were ... adjacent DNA-binding sites, a coiled-coil STAT protein all-alpha domain, which is implicated in other protein–protein interactions, a STAT protein DNA-binding domain and an SH2 doma...

Ngày tải lên: 16/02/2014, 09:20

... bacteria. The difference between these two mutants in bacterial clearance might reflect differences in the integrity of their outer membrane, binding activity and sensitivity of the murine comple- ment-mediated ... containing the various agents were plated on the lawn in triplicate at 37 °C for 18 h. Sensitivity was assessed by measuring the diameter of the zone of...

Ngày tải lên: 18/02/2014, 14:20

... are shown in white in the genomic gene and the cDNA. Intron size in bp, domain size in bp and domain size in amino acids are indicated at the bottom of each schematic representation of the gene, ... were in vitro translated in the presence of [ 35 S]methionine, and 5 lL of the labeled reac- tions were incubated with 2 lg of SmSmad1B-MBP-bound resin in bin...

Ngày tải lên: 18/02/2014, 16:20

... CA protein sequences. Iden- tical and similar amino acids shared by at least 50% of the isoforms are shown in black and grey, respectively. Histidine residues involved in zinc binding in the catalytic ... side of each filament (Fig. 2D), a homogenous fluorescence was observed in the cytoplasm of the cells. The staining appears to be specific of the probe sequence be...

Ngày tải lên: 18/02/2014, 16:20

... assisting the folding of cytoskeletal proteins that are actively synthesized and ⁄ or renatured under these conditions. The upregu- lation of all of these chaperones confirms the severity of the thermal ... number of genes encoding structural components of the cytoskeleton and proteins involved in contractile functions (including actin, tubulin myosin and profilin) wer...

Ngày tải lên: 18/02/2014, 16:20

... different domains of the protein, to determine the calcium-binding domain of the protein. (A) Schematic drawing of the recombinant proteins. Six GST-fused recombinant proteins containing the three ... be contained in the aggregate of the HMW proteins described above. After digestion of the side chains using deglycosylation enzymes, the intensity of the imm...

Ngày tải lên: 18/02/2014, 17:20