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Báo cáo khoa học: Identification of domains involved in the allosteric regulation of cytosolic Arabidopsis thaliana NADP-malic enzymes ppt

Báo cáo khoa học: Monomeric molten globule intermediate involved in the equilibrium unfolding of tetrameric duck d2-crystallin pdf

Báo cáo khoa học: Monomeric molten globule intermediate involved in the equilibrium unfolding of tetrameric duck d2-crystallin pdf

... concentrations. The d2-crystallin in lens was recruited during evolutionfrom argininosuccinate lyase, an enzyme involved in arginine biosynthesis derived from the urea cycle. Thesetwo proteins shared ... stabilityfor crystallins is essential in maintaining lens transparency[4]. Determining the mechanism of folding and assembly of these proteins is important for understanding how they canform stable ... further increased. The unfolding curveusing the ANS fluorescence probe suggested a multistateprocess, consistent with the results obtained by proteinintrinsic fluorescence and CD. The binding...
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Báo cáo khoa học: Cellular response to unfolded proteins in the endoplasmic reticulum of plants pptx

Báo cáo khoa học: Cellular response to unfolded proteins in the endoplasmic reticulum of plants pptx

... chain is brefeldin A-insensitive and isinhibited by the proteasome inhibitor clasto-lactacystinb-lactone, resulting in the accumulation of ricinA chains. These stabilized ricin A chains are partlydeglycosylated ... pivotal role of the UPR is to maintain ER home-ostasis. Therefore, the presence of mutated proteinsthat are unable to fold into their native conformation in the ER induces the UPR in an effort ... peptide in the 24-kDa a-zein and the 19-kDa a-zein endosperm storage proteins, respect-ively. These mutant proteins are translocated into the lumen of the ER, but remain anchored to the mem-branes...
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Báo cáo khoa học: Identification of functional domains in the formyl peptide receptor-like 1 for agonist-induced cell chemotaxis doc

Báo cáo khoa học: Identification of functional domains in the formyl peptide receptor-like 1 for agonist-induced cell chemotaxis doc

... control.Functional domains in FPRL1 Y. Le et al.774 FEBS Journal 272 (2005) 769–778 ª 2005 FEBS the involvement of multiple domains of FPR in recogni-tion of the agonist fMLF, including the first, second,and ... half of the first TM domain through the second intracellular loop of FPRL1 with the corresponding part of FPR (A). Chimera H contains the N-terminus and a segment between the fourth TM and the ... multiple domains are involved in FPRL1 interaction with its specific peptideagonists, with a prominent role for the sixth trans-membrane domain (TM) and the third extracellularloop in mediating the...
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Tài liệu Báo cáo khoa học: Identification of Ewing’s sarcoma protein as a G-quadruplex DNA- and RNA-binding protein ppt

Tài liệu Báo cáo khoa học: Identification of Ewing’s sarcoma protein as a G-quadruplex DNA- and RNA-binding protein ppt

... on the guanidini-um group of the Arg in the RGG domain in the C-terminal of EWS.Results and DiscussionSeveral DNA-binding proteins that bind to G-quadru-plex DNA have been investigated in ... nucleolin [21–27]. The RGG domain of FMRP, which is an RNA-binding protein involved in nerve cell differentiation,interacts with the G-quartet forming RNA [22–25]. In addition, the RBD and the ... that RGG3 of EWS binds mainly to the G-quadruplex.To test whether formation of the G-quadruplex isnecessary for RGG3 binding, we assayed the binding of RGG3 with Htelo in the presence of K+or...
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Tài liệu Báo cáo khoa học: Identification and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebrafish, Danio rerio docx

Tài liệu Báo cáo khoa học: Identification and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebrafish, Danio rerio docx

... all-alpha domain, STATprotein DNA-binding domain and SH2 domain of stat6, and the zinc-finger domain, leucine-zipperdomain and fork-head domain of foxp3. In addition,for stat6 and foxp3, there were ... adjacent DNA-binding sites,a coiled-coil STAT protein all-alpha domain, which isimplicated in other protein–protein interactions, aSTAT protein DNA-binding domain and an SH2domain, which binds phosphorylated ... the inducible chain of the IL-12 receptor (IL-12Rb2).Binding of IL-12 to its receptor induces signallingthrough STAT4, which further enhances IFN-c pro-duction and induces the expression of...
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Tài liệu Báo cáo khoa học: Identification of two late acyltransferase genes responsible for lipid A biosynthesis in Moraxella catarrhalis doc

Tài liệu Báo cáo khoa học: Identification of two late acyltransferase genes responsible for lipid A biosynthesis in Moraxella catarrhalis doc

... bacteria. The difference betweenthese two mutants in bacterial clearance might reflectdifferences in the integrity of their outer membrane,binding activity and sensitivity of the murine comple-ment-mediated ... containing the various agents were plated on the lawn in triplicate at37 °C for 18 h. Sensitivity was assessed by measuring the diameter of the zone of growth inhibition in two axes, and the ... [25], and Schwin-gel et al. found the lgt6 gene involved in the initialassembly of the LOS [26]. However, for lipid Abiosynthesis of the M. catarrhalis LOS, only an lpxAgene encoding UDP-GlcNAc...
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Tài liệu Báo cáo khoa học: Identification and characterization of an R-Smad ortholog (SmSmad1B) from Schistosoma mansoni pdf

Tài liệu Báo cáo khoa học: Identification and characterization of an R-Smad ortholog (SmSmad1B) from Schistosoma mansoni pdf

... areshown in white in the genomic gene and the cDNA. Intron size in bp, domain size in bp and domain size in amino acids are indicated at the bottom of each schematic representation of the gene, ... were in vitro translated in the presence of [35S]methionine, and 5 lL of the labeled reac-tions were incubated with 2 lg of SmSmad1B-MBP-boundresin in binding buffer overnight at 4 °C. The ... structure in which the female worm resides for mating, the upregulation of SmGCP by TGFb indicates a role in worm pairingand reproduction. Together with the recent report of Smad involvement in mammalian...
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Tài liệu Báo cáo khoa học: Identification, sequencing, and localization of a new carbonic anhydrase transcript from the hydrothermal vent tubeworm Riftia pachyptila docx

Tài liệu Báo cáo khoa học: Identification, sequencing, and localization of a new carbonic anhydrase transcript from the hydrothermal vent tubeworm Riftia pachyptila docx

... CA protein sequences. Iden-tical and similar amino acids shared by at least 50% of the isoforms are shown in black and grey, respectively. Histidine residues involved in zinc binding in the catalytic ... side of eachfilament (Fig. 2D), a homogenous fluorescence wasobserved in the cytoplasm of the cells. The stainingappears to be specific of the probe sequence because the staining is clear with the ... transcript, the relativequantification result was obtained by comparing the level of expression in each tissue with the level of expression in the calibrator tissue, with the latter being chosen as the...
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Tài liệu Báo cáo khoa học: Identification of differentially expressed genes of the Pacific oyster Crassostrea gigas exposed to prolonged thermal stress docx

Tài liệu Báo cáo khoa học: Identification of differentially expressed genes of the Pacific oyster Crassostrea gigas exposed to prolonged thermal stress docx

... assisting the folding of cytoskeletal proteins that are actively synthesizedand ⁄ or renatured under these conditions. The upregu-lation of all of these chaperones confirms the severity of the thermal ... number of genes encoding structural components of the cytoskeleton and proteins involved in contractilefunctions (including actin, tubulin myosin and profilin)were differentially expressed in C. ... other chaperones, the chapero-nin-containing TCP1 (subunit 7, isoform b) presented the same expression pattern in response to heat stress in our study. This complex, involved in folding actin,tubulin...
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Tài liệu Báo cáo khoa học: Identification of a novel matrix protein contained in a protein aggregate associated with collagen in fish otoliths pdf

Tài liệu Báo cáo khoa học: Identification of a novel matrix protein contained in a protein aggregate associated with collagen in fish otoliths pdf

... different domains of the protein, to determine the calcium-binding domain of the protein. (A) Schematic drawing of the recombinant proteins. SixGST-fused recombinant proteins containing the three ... be contained in the aggregate of the HMW proteins described above. After digestion of the side chains using deglycosylation enzymes, the intensity of the immunoreactive band in the HMW region ... secreted into the extracellu-lar matrices of the inner ear. In the otoliths, they areboth localized in the ring-like structures. These find-ings identify for the first time proteins with these...
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