Báo cáo khoa học: Control of transferrin expression by b-amyloid through the CP2 transcription factor pdf

... and 3). The presence of CP2 in the protein–DNA complex was verified by adding CP2 antibody. Addition of the CP2 antibody supershifted a portion of the CP2 com- plex (lane 6), but use of IgG as ... that CP2 is able to activate the transcription of transferrin. The transferrin proximal promoter contains a CP2- response element To determine the responding...

Ngày tải lên: 23/03/2014, 03:20

... this assumption. On the other hand, the absence of the inhibitor in free follicle cultures, may be due to the lack of cellular proliferation, absent in this type of culture. These facts support the hypothesis ... K, on the 105000·g supernatant from the thyroid monolayer cell culture. None of these proteolytic enzymes affected the inhibitory power. These results were co...

Ngày tải lên: 30/03/2014, 14:20

... some transcription factors, either at the level of their activa- tion (mainly by their ability to bind DNA) or at the level of their expression (mRNA or protein). In the lat- ter case, the specific ... presence of amino acid(s) resulted in inhibition of the DNA binding of the involved transcription factors. By contrast, the presence of amino acid may also re...

Ngày tải lên: 07/03/2014, 00:20

... determination of flux control coefficients by PFK, PK and LDH. The role of the las operon is dis- cussed on the basis of the distribution of flux control for PFK, PK and LDH. Results PFK has no control ... and the pyruvate bran- ches. The sum of control coefficients for the three enzymes individually was comparable with the control coefficient found for the...

Ngày tải lên: 19/02/2014, 17:20

... intermediate by the unique action of the serpin, whereby the RCL of the serpin inserts into the major A b -sheet causing the protease to be rapidly translo- cated from the top to the bottom of the inhibitor ... polymers of serpins, where the insertion of another molecule’s RCL takes place [8]. Both of these result in the irreversible inactivation (generally...

Ngày tải lên: 20/02/2014, 02:21

... homology 1 domain of all Smads binds to importin-b in the case of Smad3 and importin-a in the case of Smad4, while mutation of the NLS blocks the ability of these proteins to enter the nucleus [95–98]. ... established through the nuclear pore with high Ran–GDP concentrations in the cytoplasmic phase of the pore which gradually decrease along the pore [101]. In...

Ngày tải lên: 07/03/2014, 01:20

... achieved. The next step is to prove their functionality and to characterize them by the presence of specific chromatin signatures. The results of the first attempts have been published during the last ... diverse. This allows the conservation of loci to serve as a good predictor of occupancy by the receptor in vivo [47]. Furthermore, the variety of GRE sequences pr...

Ngày tải lên: 28/03/2014, 22:21

... reference level for these parental enzymes. On the other hand, compared to LAC3, as the steady-state kinetic parame- ters for all the enzymes are of the same order of mag- nitude, the ratio of the total ... preserved the integrity of domain D1 and the very end part of domain D3, two regions that interact precisely in the natural laccase fold (Fig. 5). Because of...

Ngày tải lên: 18/02/2014, 06:20

... affect sterol biosynthesis dur- ing the formation of a mating projection. Nevertheless, the phenotypes of mutants defective in ergosterol synthesis clearly demonstrate the importance of sterols for ... been suggested that the degree of sterol biosynthesis may increase in response to pheromone [28]. To test this hypothesis, we analyzed sterols from cells grown in the presence...

Ngày tải lên: 18/02/2014, 13:20

... cysteine and the P-loop in the structures of PhyAsr. Table S3. Least squares superposition of main chain atoms of the P-loop (HCX 5 RS ⁄ T) of PTP structures determined in the absence of an active ... S4). The average B-factors of the P-loop residues in the unoxidized and oxidized structures were 14.3 A ˚ 2 and 15.4 A ˚ 2 , respectively. This is 4–5 A ˚ 2 lower than...

Ngày tải lên: 18/02/2014, 18:20