Báo cáo khoa học: Fragile X-related protein FXR1 controls posttranscriptional suppression of lipopolysaccharide-induced tumour necrosis factor-a production by transforming growth factor-b1 doc

... induction of TNF via FXR1 FEBS Journal 277 (2010) 2754–2765 ª 2010 The Authors Journal compilation ª 2010 FEBS 2765 Fragile X-related protein FXR1 controls post- transcriptional suppression of lipopolysaccharide-induced tumour ... lipopolysaccharide-induced tumour necrosis factor-a production by transforming growth factor-b1 Tarnjit K. Khera 1 , Andrew...

Ngày tải lên: 22/03/2014, 21:21

... investi- gation of the chemical modifications of OBP as a result of incubation with large excesses of HNE and evaluation of their effects on the binding properties of the protein; (c) determination of OBP ... pT7-7 and the expression of the protein was realized as reported above for the recombinant forms of porcine and bovine OBP. The purification of the protein was obt...

Ngày tải lên: 23/03/2014, 10:20

... induced by isopropyl thio-b- D -galactoside and the overexpressed protein was finally purified by SP ion-exchange chromatography [24]. The purity of the protein was verified by SDS/PAGE. Protein concentration ... The extent of refolding was calculated by taking the ratio of the activity of the refolded enzyme to the activity of the same amount of native enzyme. Fluoresc...

Ngày tải lên: 23/03/2014, 12:20

... molecular docking. The computational approach allowed first, the creation of binary complexes of the natural pterin cosubstrate BH 4 and the catalytic domain of the enzymes, followed by the docking of ... substrate specificity of the enzymes. In a separate set of docking experiments, we modeled the inhibition of PAH and TH by catecholamine end products to explain the reduced s...

Ngày tải lên: 31/03/2014, 07:20

... summarize the properties of the three protein classes, with the main focus being on RNA chaperones and annealer proteins. Specifically binding proteins A specific protein cofactor binds to its RNA ... increase of the system drives binding of the peptide to the RNA (and thus, indirectly, the acceleration of annealing). However, the extent of decrease of annealing acceleration cau...

Ngày tải lên: 14/02/2014, 19:20

... the MAPKs were produced as GST-lofMPK3 and GST- lofMPK6. However, when lofMPK3 and lofMPK6 proteins were tested for phosphorylation with OXI1, no phosphorylation of lofMPK3 or lofMPK6 was observed (Fig. ... interaction) l MPK6 binds to PTI1-4 by pull down (View interaction) l PTI1-4 and MPK3 phosphorylate by protein kinase assay (View interaction) l OXI1 phosphorylates OXI1 by prot...

Ngày tải lên: 14/02/2014, 19:20

... MLL1 recruitment to chromatin results in the methylation of H3K4 by the SET domain of MLL1, an activity that is regulated in part by a core complex of proteins that includes WDR5, RbBP5, Ash2L and DPY-30 ... portion of the MLL1 TAD bound to the KIX domain of the cAMP response element-binding (CREB) binding protein (CBP) [23], a peptide from the Win motif of MLL1 bound to the...

Ngày tải lên: 16/02/2014, 14:20

... specific inhibitor of protein disulfide isomerase Anna-Riikka Karala and Lloyd W. Ruddock Biocenter Oulu and Department of Biochemistry, University of Oulu, Finland Introduction Protein disulfide ... the specificity of bacitracin for PDI nor the detailed mech- anisms of inhibition of PDI have been investigated. Furthermore, since the original reporting of the inhibi- tion of P...

Ngày tải lên: 16/02/2014, 14:20

... molecules of the viral envelope polyprotein, which are embedded in the membrane. Thus, condensation of the capsid drives its coating by an envelope polyprotein-containing lipid bilayer. As a result of ... poly- merization of Gag occurs mainly through the forma- tion of an intermediate layer of cup-shaped structures, each made of one hollow hexameric ring of CA domains placed...

Ngày tải lên: 18/02/2014, 06:20

... mature capsid of human immunodeficiency virus, HIV-1, is formed by the assembly of copies of a capsid protein (CA). The C-terminal domain of CA, CTD, is able to homodimerize and most of the dimerization ... coherence; MA, matrix protein of HIV-1; NC, nucleocapsid protein; NTD, N-terminal domain of CA, comprising residues 1–145 of the intact protein; NYAD-1, an improved...

Ngày tải lên: 18/02/2014, 06:20