Báo cáo khoa học: AdipoR2 is transcriptionally regulated by ER stress-inducible ATF3 in HepG2 human hepatocyte cells pdf

... analyzed in ER stress-induced HepG2 cells determined by western blot. Table S2. Densitometric values of proteins analyzed in HepG2 cells by western blot. Table S3. Statistical analysis of arbitrary ... activity. This adipokine has amelio- rative effects on insulin resistance in peripheral tissues, and plays a central role in the regulation of energy Keywords AdipoR2; A...

Ngày tải lên: 22/03/2014, 21:21

... streptozotocin (STZ) in rodents, which degenerates pancreatic insulin-producing b -cells [2]. This model is characterized by decreased plasma insulin levels, severe hyperglycaemia and alterations in insulin-dependent signal ... performed to distinguish between perivenous and periportal hepatocytes [30]. UPFK-2-overexpressing hepatocytes were predominantly located in the perivenous...

Ngày tải lên: 30/03/2014, 01:20

... For phosphatidylserine determination, cells were washed with binding buffer and incubated with Annexin V-FITC for 15 min at room temperature. Cells were deposited immedi- ately in poly(l-lysine) 19 coated ... molecules. Herein, we report that parameters of iron metabolism were regulated in a coordinated-fashion in a human hepatoma cell line (HepG2 cells) after iron loading, le...

Ngày tải lên: 19/02/2014, 16:20

... fate of creatine that is being synthesized by tumor cells could not be determined precisely. Furthermore, there were no significant differences in ornithine and creatinine content in the blood ... important one in tumor cells is the conversion of ornithine to putrescine, which is the precursor molecule of polyamines such as spermi- dine and spermine. This reaction is cataly...

Ngày tải lên: 07/03/2014, 04:20

... neokyotorphin stimulated proliferation, it had no effect in medium containing no fetal bovine serum. Thus, in L929 cells, neokyotorphin does not induce mitosis, rather it acts by enhancing the effect ... (5000–8000 cells per well). After 18 h of subculture, fetal bovine serum-supplied medium was replaced by fetal bovine serum- free medium containing test substances. In control sa...

Ngày tải lên: 07/03/2014, 11:20

... 2-vinylpiridine at room temperature for 60 min. In a typical experiment, 0.15 mL of supernatant was treated with 3 lL of undiluted 2-vinyl- pyridine. Nine microliters of triethanolamine were ... (1991) Reversal of age- related increase in brain protein oxidation, decrease in enzyme activity, a nd loss in temporal an d s patial m emory by chronic administration of the s pin trapping...

Ngày tải lên: 16/03/2014, 18:20

... as determined by staining with nigrosin. Caco-2 cells were cultured in minimal essential medium supplemented with 2mm glutamine, 1% nonessential amino acids and 10% N. Obermajer et al. Cystatin-loaded ... with anti-cytokeratin monoclonal IgG were added and the cells were incubated for different time periods. To determine specific uptake, MCF-10A neoT and Caco- 2 cells were plated eit...

Ngày tải lên: 23/03/2014, 07:20

... ring A of genistein. Therefore, the recognition of unique binding sites in HSA by genistein and warfarin is due to significant structural differences in ring B and such a characteristic binding ... strength on binding of genistein– HSA interaction To determine whether ionic interactions play a role in the genistein–HSA interaction, the ionic strength of the buffer was increased by...

Ngày tải lên: 23/03/2014, 11:20

... FEBS peripheral regions of the fibrinogen molecule were his- torically named D-domains. However, it was discov- ered by X-ray analysis that there were two distinct bC- and cC-domains in the peripheral ... BbArg14 is involved in another fibrin poly- merization site localized at the N-terminal fragment of the fibrin Bb-chain. This site is operational before fibrinopeptide B is split off...

Ngày tải lên: 30/03/2014, 03:20

... The Netherlands 7 Anaesthsiologist-intensivist, Department of Intensive Care Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands 8 Internist-intensivist, Research ... Netherlands 4 Clinical Epidemiologist, Department of Clinical Epidemiology and Biostatistics, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands 5 Internist-intensi...

Ngày tải lên: 25/10/2012, 10:39