Báo cáo khoa học: Arg143 and Lys192 of the human mast cell chymase mediate the preference for acidic amino acids in position P2¢ of substrates pdf
Báo cáo khoa học: Arg143 and Lys192 of the human mast cell chymase mediate the preference for acidic amino acids in position P2¢ of substrates pdf
... conferring a strong preference for acidic amino acids at position P2¢ of the substrates. Table 1. P2¢ specificity and amino acids found in positions 40, 143 and 192 of nine different chymases. Chymase ... Lys,respectively). We therefore hypothesized that Arg143 and Lys192 of human chymase mediate the preference for acidic amino acids at position P2¢ of substrates. In order to address this question, we performed ... wehypothesized that Arg143 alone or in cooperation with Lys192 mediates the preference for acidic amino acids at position P2¢. In the present study, we tested the roles of Arg143 and Lys192 as P2¢ specificity-determin-ing...
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Tài liệu Báo cáo khoa học: Diego and friends play again Old planar cell polarity players in new positions doc
... at the top of the hierarchy and is responsible for recruiting the other transmem-brane proteins, Fz and Stbm. This is supported by the finding that in the absence of fmi negligible amounts of ... PCP protein localization in the developing wing and eye. (A) During the initial phase of pupal wing development [up to 24 h after prepupa formation (APF)] the protein products of the core PCP ... activity of other proteins involved in intercellular signaling. The best candidates as targets of Fj are the atypicalcadherins Ds and Ft, which have been shown to actdownstream of fj [16]. The analysis...
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Tài liệu Báo cáo khoa học: Structure and function of active chromatin and DNase I hypersensitive sites pdf
... mammalian cells [153]. Phosphorylation of histone H3S10 by Pim1 kinase enables the recruitment of both MOF and P-TEFb via interactions involving the adaptor protein 14-3-3 and the bromodomain pro-tein ... varia-tion and complexity in the number of nucleosomes dis-rupted, the histone composition of the remainingnucleosomes, and the number and nature of proteincomplexes they interact with.Much has ... promoters and may therefore play a role of maintaining this class of promoter in an open confor-mation [196].Many promoters contain CG islands that formunstable nucleosomes The majority of CG...
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Tài liệu Báo cáo khoa học: miRNAs and regulation of cell signaling pptx
... by various cell signaling cascades. The insightsgained in such studies support the idea that miRNAs are involved in the highly complex network of cell signaling pathways. In this minireview, ... pathway.Some other miRNAs are also reported to be inducedby the MAPK signaling pathway. In the human B -cell line Ramos, miR-155 is induced by signaling by the B -cell receptor through the ERK and c-Jun ... fac-tor induced the expression of miR-221 and miR-222 in PC12 cells, and that this induction is dependent onsustained activation of the ERK1 ⁄ 2 pathway [20].Furthermore, the induction of miR-34a...
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Tài liệu Báo cáo khoa học: Functional and structural analyses of N-acylsulfonamidelinked dinucleoside inhibitors of RNase A ppt
... the Kmvalues is attributable to the uracil moiety binding in the same fashion [38], which could trigger the initialbinding of both substrates. In UpG, the binding of the guanine moiety is deterred by ... puckering in one of its alternativeconformations. The pseudorotation angles for the uridine of N-acyl-sulfonamide 7 were found in both the C3¢-endo (N)conformation and the O4¢-endo conformation, ... studies of the structure and binding sites of enzymes. 8. Inhibitor binding toribonucleases. J Mol Biol 45, 491–511.33 Ui N (1971) Isoelectric points and conformation of proteins. I. Effect of urea...
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Tài liệu Báo cáo khoa học: Active and regulatory sites of cytosolic 5¢-nucleotidase doc
... [1]. The transfer of phosphate can lead to phosphorylation of inosine,guanosine and a number of their analogues [2]. There-fore, in addition to being involved in regulation of purine intracellular ... only the amino acid residues involved in the binding of the purine base were identified, while the ribose moiety was completely disordered [11].Mutation of two residues involved in the binding of adenine ... Met in cN-III and by Ala or Gly in the otherenzymes. Therefore, the two phosphotransferases havea bulkier amino acid in this position compared to the other four enzymes, which have amino acids...
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Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx
... has a Gly in the position of the proposed catalytic Asp of HPRT. In PRTFDC1, a water molecule at the position of the aspartic acid side chain position in HPRT might beresponsible for the low activity ... solutions. The ligandbound was interpreted as GMP because the N2 of the base makes a hydrogen bond to a main chain car-bonyl. Binding of a xanthine base from XMP in the same position, would lead to the ... N7 (Fig. 1C). The 2¢OH of the ribose is hydro-gen bonded to the main chain and the side chain of Asp142 (Fig. 1C). Both hydroxyl groups of the riboseare involved in coordinating a putative...
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Tài liệu Báo cáo khoa học: Enzymatic and electron paramagnetic resonance studies of anabolic pyruvate synthesis by pyruvate: ferredoxin oxidoreductase from Hydrogenobacter thermophilus doc
... coupledassay. In the presence of the reduced Fd1 and acetyl-CoA, the HE-TPP radical intermediate was formed(Figs 3F and 4B), indicating the intermediacy of the HE-TPP radical in both the oxidative and ... CO2 and the carboxylgroup of 2-oxoglutarate [44], and therefore interfereswith the detection of [14C] pyruvate. Instead, the rate of pyruvate formation was determined by monitoring the LDH-coupled ... under-standing of the metabolism of H. thermophilus. Indeed,this would enable the kinetic analysis of the POR reac-tions in both directions. In particular, the reaction rateunder physiological intracellular...
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Tài liệu Báo cáo khoa học: Expression and secretion of interleukin-1b, tumour necrosis factor-a and interleukin-10 by hypoxia- and serum-deprivation-stimulated mesenchymal stem cells Implications for their paracrine roles ppt
... paracrine manner under ischaemic conditions.Taken together, these findings may improve understanding of the cellu-lar and molecular basis of the anti -in ammatory and paracrine effects of mesenchymal ... enhances the transcription and secretion of IL-10, which may be an importantmediator of the cells’ paracrine anti-fibrotic effects.These findings help to improve our understanding of the cellular and ... IL-10 for 24 h. DNA synthesis at 24 h was measured using a BrdUELISA kit. Briefly, the cells were incubated for 4 h at37 °C with 20 lLÆwell)1 of BrdU. The supernatant was thenremoved and the cells...
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Tài liệu Báo cáo khoa học: Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 docx
... are used as inter-mediates in several industries. d -amino acids areinvolved in the synthesis of antibiotics, pesticides,sweeteners and other biologically active peptides.l -amino acids are used ... analysis of substrate on the active site of HYDJs The substrate binding pocket in the active site was inves-tigated based on the homology model to obtain moreinformation on substrate binding in ... hydantoinases involved screening and iso-lating naturally occurring enzymes possessing hydan-toin-hydrolyzing activity from microbes, and usingthem to produce optically pure amino acids [4,16–18].The...
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Từ khóa: báo cáo khoa học mẫubáo cáo khoa học y họcbáo cáo khoa học sinh họcbáo cáo khoa học nông nghiệpbáo cáo khoa học lâm nghiệpbáo cáo khoa học thủy sảnchuyên đề điện xoay chiều theo dạngMột số giải pháp nâng cao chất lượng streaming thích ứng video trên nền giao thức HTTPNghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namBiện pháp quản lý hoạt động dạy hát xoan trong trường trung học cơ sở huyện lâm thao, phú thọGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDEPhát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Tìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinThiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíBT Tieng anh 6 UNIT 2Tăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)chuong 1 tong quan quan tri rui roTrách nhiệm của người sử dụng lao động đối với lao động nữ theo pháp luật lao động Việt Nam từ thực tiễn các khu công nghiệp tại thành phố Hồ Chí Minh (Luận văn thạc sĩ)BÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘI