Báo cáo khoa học: Arg143 and Lys192 of the human mast cell chymase mediate the preference for acidic amino acids in position P2¢ of substrates pdf

... conferring a strong preference for acidic amino acids at position P2¢ of the substrates. Table 1. P2¢ specificity and amino acids found in positions 40, 143 and 192 of nine different chymases. Chymase ... Lys, respectively). We therefore hypothesized that Arg143 and Lys192 of human chymase mediate the preference for acidic amino acid...

Ngày tải lên: 22/03/2014, 21:21

... at the top of the hierarchy and is responsible for recruiting the other transmem- brane proteins, Fz and Stbm. This is supported by the finding that in the absence of fmi negligible amounts of ... PCP protein localization in the developing wing and eye. (A) During the initial phase of pupal wing development [up to  24 h after prepupa formation (APF)] the pr...

Ngày tải lên: 20/02/2014, 01:20

... mammalian cells [153]. Phosphorylation of histone H3S10 by Pim1 kinase enables the recruitment of both MOF and P-TEFb via interactions involving the adaptor protein 14-3-3 and the bromodomain pro- tein ... varia- tion and complexity in the number of nucleosomes dis- rupted, the histone composition of the remaining nucleosomes, and the number and nature of p...

Ngày tải lên: 14/02/2014, 18:20

... by various cell signaling cascades. The insights gained in such studies support the idea that miRNAs are involved in the highly complex network of cell signaling pathways. In this minireview, ... pathway. Some other miRNAs are also reported to be induced by the MAPK signaling pathway. In the human B -cell line Ramos, miR-155 is induced by signaling by the B -cell...

Ngày tải lên: 14/02/2014, 19:20

... the K m values is attributable to the uracil moiety binding in the same fashion [38], which could trigger the initial binding of both substrates. In UpG, the binding of the guanine moiety is deterred by ... puckering in one of its alternative conformations. The pseudorotation angles for the uridine of N-acyl- sulfonamide 7 were found in both the C 3 ¢-endo...

Ngày tải lên: 14/02/2014, 22:20

... [1]. The transfer of phosphate can lead to phosphorylation of inosine, guanosine and a number of their analogues [2]. There- fore, in addition to being involved in regulation of purine intracellular ... only the amino acid residues involved in the binding of the purine base were identified, while the ribose moiety was completely disordered [11]. Mutation of two...

Ngày tải lên: 15/02/2014, 01:20

... has a Gly in the position of the proposed catalytic Asp of HPRT. In PRTFDC1, a water molecule at the position of the aspartic acid side chain position in HPRT might be responsible for the low activity ... solutions. The ligand bound was interpreted as GMP because the N2 of the base makes a hydrogen bond to a main chain car- bonyl. Binding of a xanthine...

Ngày tải lên: 15/02/2014, 01:20

... coupled assay. In the presence of the reduced Fd1 and acetyl- CoA, the HE-TPP radical intermediate was formed (Figs 3F and 4B), indicating the intermediacy of the HE-TPP radical in both the oxidative and ... CO 2 and the carboxyl group of 2-oxoglutarate [44], and therefore interferes with the detection of [ 14 C] pyruvate. Instead, the rate of pyruvat...

Ngày tải lên: 16/02/2014, 09:20

... paracrine manner under ischaemic conditions. Taken together, these findings may improve understanding of the cellu- lar and molecular basis of the anti -in ammatory and paracrine effects of mesenchymal ... enhances the transcription and secretion of IL-10, which may be an important mediator of the cells’ paracrine anti-fibrotic effects. These findings help to improve our...

Ngày tải lên: 18/02/2014, 04:20

... are used as inter- mediates in several industries. d -amino acids are involved in the synthesis of antibiotics, pesticides, sweeteners and other biologically active peptides. l -amino acids are used ... analysis of substrate on the active site of HYD Js The substrate binding pocket in the active site was inves- tigated based on the homology model to obtain more i...

Ngày tải lên: 18/02/2014, 08:20