Báo cáo khoa học: Loss of sense transgene-induced post-transcriptional gene silencing by sequential introduction of the same transgene sequences in tobacco pot
... Loss of sense transgene- induced post-transcriptional gene
silencing by sequential introduction of the same transgene
sequences in tobacco
Sayaka Hirai
1
, Kouta ... and the 5¢-termini of the NtFAD3
transgene cDNAs were distributed in the same region
of the transgene sequences, suggesting that the NtFAD3
transgene was transcribed und...
... substantially
smaller in sense plants than in antisense and control
plants: 5 days after infection, the diameter of the
lesions was about 44 mm in the leaves of antisense
plants, but only 17 mm in the leaves of the ... obtained
from the Internet.
A phylogenetic tree was constructed online using the
default settings of the web-based alignment tool multa-
lin (...
... with
benzyladenine resulted in staining of 76% of the BY- 2
cells. These results indicate that the AaGlucan does
not induce cell death in the BY- 2 cells under the condi-
tions employed.
The production of ... and the periodate-sensitive
residues are essential for the chitinase inducing activity
of AaGlucan.
Cellular responses induced by the AaGlucan
elicitor
T...
... each concept contains 7
fields including lexical entries in Chinese, Eng-
lish gloss, POS tags for the word in Chinese and
English, and a definition of the concept including
its category and ... dis-
ambiguation in these systems.
We therefore propose to generate a bilingual
frame semantics mapping (word sense diction-
ary), simulating the “semantic map” in a bilin-
gual...
... anti-SigF domain of MursiF, the
RsbW ⁄ kinase domain (WKD), shows 40% similarity
to B. subtilis RsbW, a serine kinase belonging to the
GHKL family of kinases. This family of kinases is
defined by the presence ... common domain, RsbU ⁄ phosphatase domain (
). The sequences homologous to other two domains,
the RsbW domain (
) and the RsbV domain ( ), of Rv1364c (A) exist...
... preceded/succeeded sense by all the
specific words in the article of the specific
abbreviation instance.
4 Implementing the OS Hypothesis
As mentioned above, the basic assumption of the
OS hypothesis ... written styles. Based on this
hypothesis, many terms repeat themselves with the
same meaning in all their occurrences. Within the
context of determining...
... X
6
remains the same
upon engineering of the SBS of XBS, the results
obtained in the previous screening experiment directly
Table 1. The effect of genetic engineering of the secondary bind-
ing site ... enzyme in a final volume of 7.80 mL
after 10 min of pre-incubation. During incubation, the sam-
ples were continuously stirred. For OSX, the reaction was
termi...
... were increased in TG mice by the HF diet.
mRNA expression of adipogenic genes in WAT of
TG mice
We measured the amounts of PGD
2
in WAT after
6 weeks of the HF diet. WAT of TG (S55) mice con-
tained ... we show, for the first time, that PGD
2
is
involved in the activation of adipogenesis and regula-
tion of insulin sensitivity in vivo. Further characteriza-...
... Interestingly, in
all WhiB proteins except WhiB2, the amino acid imme-
diately after the C-terminal cysteine of the CXXC
motif is proline (Fig. S6). In WhiB2 the absence of
proline and therefore of the ... for
resistance to intracellular killing, the scavenging of free
radicals and the reactivation of degenerated proteins
during infection by a family of protein...
... from the entire cod-
ing sequence of the MCPIP gene. The coding sequence
lacking the PIN domain was obtained in two steps. First,
the fragment of 423 bp situated in front of the PIN domain
was ... the MCPIP PIN domain. Multiple
sequence alignment of target ⁄ template PIN domain
sequences was performed using clustalx [8].
Generation of genetic constructs
The co...