Báo cáo khoa học: Crystal structure of the halotolerant c-glutamyltranspeptidase from Bacillus subtilis in complex with glutamate reveals a unique architecture of the solvent-exposed catalytic pocket docx

... deeply grooved catalytic pockets in the asymmetric unit, and the glutamate- binding modes are identical to each other (Fig. 2A) . The a- carboxyl and a- amino groups of the bound glutamate are at the bottom of ... report the crystal structure of B. subtilis GGT in complex with glutamate, a product of the enzymatic reaction, at 1.95 A ˚ resolutio...

Ngày tải lên: 22/03/2014, 21:20

... 13% of the accessible surface area of each dimer. Neighboring tet- ramers came into loose contact with each other in the crystal packing. Similar molecular arrangements have been found in the crystal ... bound to the metal, in the company of a water oxygen, from the apical positions. The manga- nese was only 0.06 A ˚ out of the equatorial plane (Table...

Ngày tải lên: 14/02/2014, 22:20

... medi- ate interaction of the two b-barrels, formation of the catalytic machinery and structures required for binding of the main chain of the substrate peptide and proper positioning of the scissile ... main chain of the catalytic domain (B-chain) of DESC1 is well defined. Only a few side chains on the surface of the mole- cule are partially undefined in...

Ngày tải lên: 19/02/2014, 00:20

... concentra- tion justifies the presence of acetate in the crystal structure. The binding of acetate in the active site is a further indication that the enzyme may be involved in deacetylation because acetate ... present the crystal structure of BcZBP at 1.8 A ˚ resolution. The N-terminal part of the 234 amino acid protein adopts a Rossmann fold whereas...

Ngày tải lên: 19/02/2014, 00:20

... l-glutamine, with that of Erwinia carotovora asparaginase, which has substrates other than l-glutamine, shows that Mglu has a larger substrate-binding pocket that prevents the binding of l-asparagine ... the structure of E. carotovora asparaginase in complex with l -glutamate (PDB 2hln) [18]. Mglu binds l -glutamate in an extended form (Fig. 7A) with a contact...

Ngày tải lên: 06/03/2014, 09:22

... of the interactions of papain with loop L6 of chagasin and loop L2 of cysta- tin B. (C) Zoom -in view of the interactions of papain with loop L4 of chagasin and the N-terminal segment of cystatin ... Od1 atom of N64e forms a water-mediated contact with the main-chain N atom of G68, and the main-chain nitrogen of G66 of chagasin forms a water-mediat...

Ngày tải lên: 16/03/2014, 04:20

... quaternary organization may be critical in creating regulatory abilities. Crystal packing Both crystal forms of TM1415 have a tetramer present in the asymmetric unit with similar packing of individ- ual ... on the structure of the eukaryotic FBPase takes a 30° rotation in the twist direction of the archaeal enzyme. In comparing the dimer orientation of...

Ngày tải lên: 16/03/2014, 11:20

... by a core DNA polymerase assembly that is attached to a sliding clamp. Although a single sliding clamp may remain attached to the polymerase during replication of the leading strand, each Okazaki ... nuclear antigen (PCNA) in eukaryotes and archaebacteria] that tether the DNA polymerase to DNA [2]. The interaction between the DNA polymerase and the sliding clamp enables...

Ngày tải lên: 16/03/2014, 16:20

... were used: the Ramachandran distance and a real-space distance. The Ramachandran distance, D, was computed by comparing the Ramachandran plots for CLIC1 and CLIC4(ext) using the equation: D ¼fðU CLIC4 À ... coordi- nates and structure factors have been deposited in the Protein Data Bank (accession code 2AHE). Ramachandran and real-space distances To locate structural changes...

Ngày tải lên: 23/03/2014, 15:21

... between bB and aB, which may arise from binding of the C-terminus of a neighboring PDZ domain in the crystal. We therefore used chain A, which does not contain anything in the ligand binding site, ... typical architecture for PDZ domains. In the structure of the peptide complex, only the last four C-terminal residues of the GluR- A are visible, and ali...

Ngày tải lên: 30/03/2014, 10:20