Báo cáo khoa học: Okadaic acid induces DNA fragmentation via caspase-3-dependent and caspase-3-independent pathways in Chinese hamster ovary (CHO)-K1 cells pot
... Okadaic acid induces DNA fragmentation via caspase-3-dependent and caspase-3-independent pathways in Chinese hamster ovary (CHO)-K1 cells Ikuko Kitazumi, Yoko Maseki, ... Chinese hamster ovary (CHO)-K1 cells and examined the effects of gene knockout and treatment with caspase-3 inhibitors. Okadaic acid (OA) is a potent inhibitor of the ser...
Ngày tải lên: 22/03/2014, 21:20
... 5 m M ascorbic acid. Ó FEBS 2003 Ascorbic acid induces conformational change in DNA (Eur. J. Biochem. 270) 3103 (TEM) image of T4 DNA in the presence of 5 m M ascorbic acid, and indicates that ... whereas the minus band at 246 nm remains essentially constant. Interestingly, the band shape in the difference spectrum resembles that of the C-form of DNA [18,19]. Discussion A...
Ngày tải lên: 23/03/2014, 21:20
... obtained using the primer pair T7 and RIB. Lanes 2, 4, 6, 8, 10, 12 and 14 show the PCR products obtained using the primer pair T7 and ADC2. Lane 15, 1 kb DNA ladder standard. (B) DNA obtained ... con- taining DNA spots (5 lg each) corresponding to inter- nal fragments of ADC or cruzipain genes. The latter (used as a control) is a housekeeping gene that encodes the main cysteine p...
Ngày tải lên: 19/02/2014, 07:20
Tài liệu Báo cáo khoa học: "Discriminative Lexicon Adaptation for Improved Character Accuracy – A New Direction in Chinese Language Modeling" pptx
... be used in the construction. That is, beginning with only characters in the lexi- con and using the training data to alter the current lexicon in each iteration. This is also an interesting direction. References Maximilian ... objective point of view since SDR evalua- tion is inevitably effected by the selected queries. 5 Conclusion and Future Work Characters together is an interest...
Ngày tải lên: 20/02/2014, 07:20
Báo cáo khoa học: A new approach for distinguishing cathepsin E and D activity in antigen-processing organelles pdf
... proteinase with a limited distribu- tion in certain cell types, including gastric epithelial cells [4], but is mainly present in cells of the immune Keywords antigen-presenting cells; cathepsin ... processing MHC II-associated invariant chain [24] in antigen processing and presentation [25,26]. CatE is also reported to be involved in antigen processing by B cells [27,28] micr...
Ngày tải lên: 07/03/2014, 09:20
Báo cáo khoa học: The lactate dehydrogenases encoded by the ldh and ldhB genes in Lactococcus lactis exhibit distinct regulation and catalytic properties ) comparative modeling to probe the molecular basis pdf
... simulate the binding equilibrium of protons in proteins have been described in detail elsewhere [43,44]. These are based on continuum electrostatic methods and Monte Carlo (MC) sampling of binding states. ... present in the parental strain and in the mutant FI9078 were due to homofunctional enzymes displaying clear differences in kinetic and reg- ulatory parameters. Therefore,...
Ngày tải lên: 16/03/2014, 05:20
Báo cáo khoa học: "An Architecture for Dialogue Management, Context Tracking, and Pragmatic Adaptation in Spoken Dialogue Systems" pot
... chitecture is distinguished from these in its treatment of discourse-level processing. Most architectures, including ours, contain mod- ules for speech recognition and natural language interpretation ... Tracking The Context Tracker maintains a record of the discourse context which it and other components can consult in order to (a) resolve dependent forms that occur in inp...
Ngày tải lên: 17/03/2014, 07:20
Báo cáo khoa học: Study of uptake of cell penetrating peptides and their cargoes in permeabilized wheat immature embryos pot
... Tat 2 peptide in delivering plasmid DNA carrying the GUS gene was studied in permeabilized wheat embryos. Being arginine rich, Tat 2 can bind anionic DNA elec- A. Chugh and F. Eudes CPPs and their cargo in ... neuropeptide galanin in the N-terminus connected with Lys13 to 14 amino acids from the wasp venom mastoparan in the C-terminus. c Source: derived from murine vascular...
Ngày tải lên: 23/03/2014, 07:20
Báo cáo khoa học: Assimilation of excess ammonium into amino acids and nitrogen translocation in Arabidopsis thaliana – roles of glutamate synthases and carbamoylphosphate synthetase in leaves ppt
... synthesis in Arabidopsis [9]. The amino acids are then translocated in the apoplasm and in the phloem via the plasma membrane-located amino acid trans- porters [10]. Glutamine and asparagine, and to ... the T -DNA insertion site, and RT-PCR analysis of transcript levels in the Arabidopsis T -DNA insertion mutants. (A) GLU2 with T -DNA inser- tion in intron 9. (B) GLT...
Ngày tải lên: 30/03/2014, 01:20
Báo cáo khoa học: A single intersubunit salt bridge affects oligomerization and catalytic activity in a bacterial quinone reductase pptx
... reactant solutions were joined in single mixing mode, using a 0.5 mL stopping syringe. FMN oxidation and reduction were measured respectively, by monitoring changes in A 453 with a KinetaScanT diode array ... present as tetramers showing packing similar to the wild-type protein (Fig. 6). Inter- estingly, inverting the position of the interaction partners in the K109D ⁄ D137K protein v...
Ngày tải lên: 30/03/2014, 01:20