Báo cáo khoa học: Multidrug efflux pumps: The structures of prokaryotic ATP-binding cassette transporter efflux pumps and implications for our understanding of eukaryotic P-glycoproteins and homologues ppt

Tài liệu Báo cáo khoa học: MicroRNA-23a promotes the growth of gastric adenocarcinoma cell line MGC803 and downregulates interleukin-6 receptor pdf

... All of the tumor types were conﬁrmedby pathologic analysis. The experiments were undertakenwith the understanding and written consent of each subject. The study methodologies conformed to the ... After incubation for 24, 48 and 72 h, the cells were incubated with 20 lL of MTT (at aﬁnal concentration of 0.5 mgÆmL)1)at37°C for 4 h. The medium was removed and the precipitated formazan wasdissolved ... on the expression of IL6R by RT-PCR and western blot, and found an inverse correlation between miR-23a and IL6R expression at both the mRNA and protein levels.To conﬁrm the direct regulation of...

Tài liệu Báo cáo khoa học: Inorganic phosphate regulates the binding of coﬁlin to actin ﬁlaments pdf

... ends, and in particular at the barbed end [23]. On the other hand, Pi has a negli-gible effect on the critical concentration for polymeriza-tion of F-actin in the presence of ATP, because of the protective ... digestions of F-actin revealed that after30 s incubation with coﬁlin at pH 8.0 the rate and the extent of actin cleavage was the same in the presence and absence of 30 mm Pi. On the otherhand, at ... in the accessibility of etheno-ADP to collisional quenchers.These changes transform the nucleotide cleft of F-actin to G-actin-like.Pi regulation of coﬁlin binding and the coﬁlin regulation of...

Tài liệu Báo cáo khoa học: Final steps in the catabolism of nicotine Deamination versus demethylation of c-N-methylaminobutyrate doc

... completing the catabolism of the pyrrolidine moiety of nicotine.Expression of the genes of these enzymes was dependent on the presence of nicotine in the growth medium. Thus, two enzymes of the nicotine ... expression of these genesdepends on the presence of nicotine in the growthmedium and we have determined the enzyme activities of the proteins. Our results demonstrate the presence of two pathways of ... 4-aminobutyrate and CH3NH2as carbon source. (A) Seven microliters of medium of a10 mL culture grown for 1 h (lanes 2 and 6), for 2 h (lanes 3 and 7), for 3 h (lanes 4 and 8), and for 4 h (lanes 5 and...

Tài liệu Báo cáo khóa học: Further insights into the assembly of the yeast cytochrome bc1 complex based on analysis of single and double deletion mutants lacking supernumerary subunits and cytochrome b pdf

... 7. The arrowslabeled (b) and (c) point to the areas of interaction between the transmembrane helix of subunit 8 and helices G and H1 of cytochrome b and between the N-terminus of subunit 8 and ... panels A and B and the relative amounts of each of the subunits determined by densitometry scanning of the stained Westernblots of the q° and CKL57 membranes are shown in panels C and D, respectively.Ó ... amounts of the subunitsdetermined by densitometry scanning of the stained gels aretabulated in Table 3. The relative amounts of cytochrome b and the mature forms of both cytochrome c1 and RieskeISP...

Tài liệu Báo cáo khoa học: Oxygen tension regulates the expression of a group of procollagen hydroxylases docx

... thus on the onehand fully support these previous ﬁndings and on the otherhand extend them by far and set them in a more generalcontext, as both a-subunits of P4h, PDI as the b-subunit of P4h ... clear twofold increase of PDI mRNA,a more moderate increase of the mRNAs for prolylhydroxylases, and did not increase the mRNA abundance of the other enzymes.Discussion Our data show that the ... generated from the pooled RNA of the normoxic (control) cultures (at the different time points) of the respective experimental series(standard cDNA). Analysis of the individual unknownstherefore yielded...

Tài liệu Báo cáo khoa học: Proteasome involvement in the degradation of the Gq family of Ga subunits pptx

... activation of Ga16 and Gaqsubunits. Therefore the lack of change in the rate of degradation cannot be due to lack of receptor-activa-tion of the alpha subunits.Given that the activation of G proteins ... immunoprecipitates and the arrowheads indicate the position of Gaq and Ga16. The molecular mass standards areindicated. The arrow indicates the position of some unspeciﬁc bands.Proteasome degradation of ... ormaturation of the protein. An increase was alsoobserved in the rate of degradation of the remainingmutant protein (20% left of the protein) vs. the wildtype (40% left of the protein) at 3 h of chase(Fig....

Tài liệu Báo cáo khoa học: Multidentate pyridinones inhibit the metabolism of nontransferrin-bound iron by hepatocytes and hepatoma cells docx

... chelators on the uptake and fate of NTBI and to investigate the potential of these chelators for therapeutic use in Fe overload diseases and cancer chemotherapy. Desferrioxamine (DFO), the only chelator ... reactionsessential for growth and proliferation, is uncertain. Hence, itis of interest to investigate the uptake and metabolism of NTBI in normal and cancer cells, and the effect of Fechelators on these ... Australia and is in accordance with the AustralianCode of Practice for the care and use of animals for scientiﬁcpurposes as well as the guidelines published by the NationalInstitutes of Health,...

Tài liệu Báo cáo khoa học: Adaptive changes in the expression of nuclear and mitochondrial encoded subunits of cytochrome c oxidase and the catalytic activity during hypoxia pptx

... switch and alters the expression of the twonuclear genome coded isoforms of CytOX V [10,32]. The regulation of genes CytOX5a and CytOX5b, coding for the two isofoms Va and Vb, parallels that of ... with the latterexpressedinanO2 and heme regulated manner.In this study therefore, we investigated how O2modulates the expression of mRNAs for CytOX subunits and also the catalytic activity of ... hypoxia on the steadystate levels of nuclear genome encoded CytOX IV and VbmRNAs. There was no change in the levels of these mRNAsin macrophages at 6 h of hypoxia, the time point at whichthere...

Tài liệu Báo cáo khoa học: "Detecting Novel Compounds: The Role of Distributional Evidence" potx

... represents a window of twowords to the left and four words to the right of the candidate noun-noun sequence. Table 5 shows the performance of the two classifiers for some of the contextual feature ... between one and four words be-fore and after the candidate compounds. We usesymbols 1 and r for left and right context, respec-tively and number to denote the window size. For example, ... areused. We evaluated the influence of context byvarying both the position and the size of the win-dow of words (i.e., parts of speech) surrounding the candidate compound. The window size param-eter...

Báo cáo khoa học: NF-jB regulates the transcription of protein tyrosine kinase Tec pdf

... critical for the survival of developing B lym-phocytes and the maturation of spleen B cells [41–43].Abnormal activation of NF-jB has been shown to bea hallmark of the development and progression of ... Tectranscription via the NF-jB signaling pathway. Theseﬁndings provide further insight into the transcriptionalregulation of the TFKs and the role of NF-jB signalingin lymphocyte development and the immune ... regulating the function of B and T lym-phocytes, as well as of other types of cells. Kitanakaet al.[12] showed that Tec is activated in both mature and immature B-lymphoid cells, and is therefore...

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