Báo cáo khoa học: Multidrug efﬂux pumps: Substrate selection

Báo cáo khoa học: "The Descent of Hierarchy, and Selection in Relational Semantics" docx

... happening in (lo- cation) or done by the kidney. Immunoglobulin staining, (D12 -Amino Acids, Peptides-, and Proteins, E05 - Investigative Techniques-) can mean either staining with immunoglobulin ... from training instances, achieving about 60% accuracy on an 18-way classiﬁcation problem using a very small training set. That approach is bottom up and requires good coverage in the trai...

Ngày tải lên : 17/03/2014, 08:20

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Báo cáo khoa học: Binding and activation of nitric oxide synthase isozymes by calmodulin EF hand pairs potx

... N-terminal oxygenase domain. The reductase domains bind NADPH, FAD, and FMN, and the oxygenase domain contains binding sites for heme, tetrahydrobiopterin (H 4 B), and the substrates l-arginine ... construct includes only the C-terminal EF hand pair including the central linker region (residues 7 6–1 48). In addition, CaMNN contains two repeats of the N-terminal EF hand pair (residues...

Ngày tải lên : 16/03/2014, 13:20

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Báo cáo khoa học: Identiﬁcation of preferred substrate sequences for transglutaminase 1 – development of a novel peptide that can efﬁciently detect cross-linking enzyme activity in the skin pot

... The CE comprises several proteins, including involucrin, loricrin, trichohyalin and small proline rich-proteins, that are preferred substrates for TGases in the skin. Of the eight TGase isozymes, four ... 3 and 5) are expressed in keratino- cytes [1 3–1 8], and are involved in cross-linking of the structural proteins to form the CE at distinct intracellu- lar sites [8,18]. In the cu...

Ngày tải lên : 23/03/2014, 06:20

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Báo cáo khoa học: Chaperone activity of recombinant maize chloroplast protein synthesis elongation factor, EF-Tu docx

... recombinant p rotein using the E CL che miluminescent development me thod with primary antibody raised against the my c epitope, which is included near the C-terminus of recombinant protein [9]. Mass ... separated using a C18 RP PepMap, 75 lm (internal diameter) column (LC P ackings, Dionex, San Francisco, CA). The standard gradient us ed was a s follows: 0 –2 min, 3% B isocratic; 2–4 0...

Ngày tải lên : 23/03/2014, 13:20

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Tài liệu Báo cáo khoa học: Enzymatic and electron paramagnetic resonance studies of anabolic pyruvate synthesis by pyruvate: ferredoxin oxidoreductase from Hydrogenobacter thermophilus doc

... acetyl-CoA, using OGOR to reduce ferredoxin as in the coupled assay. In the presence of the reduced Fd1 and acetyl- CoA, the HE-TPP radical intermediate was formed (Figs 3F and 4B), indicating the intermediacy ... acting as physiological electron donors. However, because this assay is a complex system involving four proteins, kinetic analysis was not possi- ble. The substrates, CoA and CO...

Ngày tải lên : 16/02/2014, 09:20

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Tài liệu Báo cáo khoa học: Roles of AP-2 transcription factors in the regulation of cartilage and skeletal development doc

... proline-rich and glutamine-rich N-terminus, which is important for transactivation, is shown in yellow, and contains the PY-motif (green). The helix–span–helix domain at the C-terminus shown in ... also been found in frogs and ﬁsh, and homologs occur in invertebrates. All AP-2s have a highly conserved basic helix–span–helix DNA-binding and dimerization domain at their C-terminus, and...

Ngày tải lên : 16/02/2014, 09:20

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Tài liệu Báo cáo khoa học: The two Caenorhabditis elegans metallothioneins (CeMT-1 and CeMT-2) discriminate between essential zinc and toxic cadmium docx

... binding specificity in zinc finger peptides. Inorg Chem 32, 93 7–9 40. 47 Blindauer CA (2008) Metallothioneins with unusual residues: histidines as modulators of zinc affinity and reactivity. J Inorg ... Capdevila M (1999) Replacement of terminal cysteine with histidine in the metallothionein a and b domains maintains its binding capacity. Eur J Biochem 259, 51 9–5 27. 51 Harris RK, Beck...

Ngày tải lên : 16/02/2014, 15:20

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Tài liệu Báo cáo khoa học: The alr2505 (osiS) gene from Anabaena sp. strain PCC7120 encodes a cysteine desulfurase induced by oxidative stress docx

... recorded before (bold line) and after (discontinued line) adding 0.1 m M of L-cysteine. (B) Protein–protein interactions between OsiS monomers. OsiS–OsiS interactions were detected using an E. coli two-hybrid ... molybdopterin [ 1–3 ], thiamin [4,5], the thionucleo- tides in tRNA [ 6–9 ] and the assembly of Fe-S clusters [1 0–1 5]. The ﬁrst cysteine desulfurase to be discovered wa...

Ngày tải lên : 18/02/2014, 04:20

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Tài liệu Báo cáo khoa học: Molecular defect of isovaleryl-CoA dehydrogenase in the skunk mutant of silkworm, Bombyx mori ppt

... the monomer, pointing toward FAD or its neigh- bouring monomer in the tetramer. By contrast, the side chain of the mutated residue (Val376) points toward the inside of the helix–loop–helix structure. Consequently, ... role in regu- lating all three branched-chain amino acid levels in haemolymph. Thus far, little is known about amino acid catabolism in insects. It is hoped that the sku...

Ngày tải lên : 18/02/2014, 04:20

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Tài liệu Báo cáo khoa học: An ecdysteroid-inducible insulin-like growth factor-like peptide regulates adult development of the silkmoth Bombyx mori docx

... KS (1993) Plasma insulin, C-peptide, and proinsulin concentrations in obese and nonobese individuals with varying degrees of glucose tolerance. J Clin Endocrinol Metab 76, 4 4–4 8. 25 Baker J, ... of insulin-like growth factors in embryonic and postnatal growth. Cell 75, 7 3–8 2. 26 Holt RIG (2002) Fetal programming of the growth hor- mone–insulin-like growth factor axis. Trends Endoc...

Ngày tải lên : 18/02/2014, 13:20

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Báo cáo khoa học: Multidrug efflux pumps: Substrate selection in ATP-binding cassette multidrug efflux pumps – first come, first served? doc

Báo cáo khoa học: "The Descent of Hierarchy, and Selection in Relational Semantics" docx

Báo cáo khoa học: Binding and activation of nitric oxide synthase isozymes by calmodulin EF hand pairs potx