Báo cáo khoa học: Rapamycin inhibits lipopolysaccharide induction of granulocyte-colony stimulating factor and inducible nitric oxide synthase expression in macrophages by reducing the levels of octamer-binding factor-2 doc

... 2010 The Authors Journal compilation ª 2010 FEBS Rapamycin inhibits lipopolysaccharide induction of granulocyte-colony stimulating factor and inducible nitric oxide synthase expression in macrophages ... an inhibitory effect of rapamycin on the lipopolysac-charide (LPS)-induced expression of both inducible nitric oxide synthase (iNOS) and granulocyte-colony stimulating factor (G-CSF) in macrophages and ... the LPS-induced increase in iNOS and G-CSF mRNA levels and iNOS and G-CSF protein levels. Blocking of mTOR-signaling using a dominant-negative mTOR expression plasmid resulted in inhibition of...

Báo cáo khoa học: Tumor suppressor p16INK4a ) modulator of glycomic proﬁle and galectin-1 expression to increase susceptibility to carbohydrate-dependent induction of anoikis in pancreatic carcinoma cells ppt

... relationship between galectin-1 and the expression of the p16INK4aprotein could bedelineated. In this sense, the p16INK4a-dependentincrease of the presentation of binding sites by reduceda2,6-sialylation ... redu-cing the extent of binding of labeled galectin-1 to the cell surface (data not shown). These results imply that the a5b1-integrin is a binding partner of anoikis-indu-cing galectin-1.S. ... note, these two cell lines giveinsight into the speciﬁcity of the effect as a result of their differences in the status of the retinoblastomatumor-suppressor gene, pRb. Increased galectin-1expression...

Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx

... full-lengthv-KIND; DKIND1, deletion of KIND1;DKIND2, deletion of KIND2; DKIND1 + 2,deletion of both KIND1 and KIND2; DRasN,deletion of RasN; DGEF, deletion of RasGEF; KIND1, KIND1 domain; KIND2,KIND2 ... lackingKIND2 (D KIND2 and DKIND1 + 2) failed to bindto GST-CD2 (Fig. S2A). This indicates that the KIND2 domain of v-KIND is involved in the interac-tion with the CD2 region of MAP2. Furthermore,a ... that the coresequence critical for the speciﬁc MAP2 binding resideswithin the 32 residues (amino acids 456–487) of the v-KIND KIND2 domain.To examine whether the MAP2 binding core site of v-KIND...

Tài liệu Báo cáo khoa học: Mixed-type noncompetitive inhibition of anthrax lethal factor protease by aminoglycosides ppt

... binding. Only one ion pair seems to beinvolved in the binding process, which suggests the presence of a singlebinding site. Combining the results of our substrate competition studieswith the ... governing the inhibition of the lethal factor protease by aminoglycosides. Forexample, the kinetic mechanism of inhibition by neo-mycin B has been reported as being competitive with the substrate ... substrate, in conjunctionwith the mixed-type noncompetitive inhibition patternseen with neomycin B and other inhibitors, suggesteither the presence of two distinct binding sites or the involvement of...

Tài liệu Báo cáo khoa học: Fatty acid synthesis Role of active site histidines and lysine in Cys-His-His-type b-ketoacyl-acyl carrier protein synthases ppt

... reaction.An interesting aspect of the H298E mutation is the orientation of the T300 ⁄ T302 side chains. Both havebeen implicated in the binding of ACP [2], probably by donating hydrogen bonds to one of ... all pointto the orientation of H333 and its hydrogen bonddonor properties as being the cornerstone of obtaining and ⁄ or retaining residual decarboxylase activity. The emerging picture of well-deﬁned ... the vicinity of the active site (Fig. 2). Three of the six cation lig-ands are main-chain oxygen atoms, and three are the side-chain oxygen atoms of N296, E342 and S387. The glutamic acid and asparagine...

Tài liệu Báo cáo khóa học: Non-speciﬁc depolymerization of chitosan by pronase and characterization of the resultant products pptx

... linear polymer, its freeze-drying results in the aggregation of the chains by inter- and intrachain hydrogenbonding network resulting in the formation of particle-like structure [1]. ESEM of ... 3600–3000 cm)1 in the spectra of bothchitosan and LMWC indicated the absence of free -OHgroups and the involvement of both -OH… 3and -CH2OH…6 in intra- and intermolecular hydrogen bonding(Fig. ... beadcharacteristics, in which increases in molecular mass and DA resulted in efﬁcient loading and minimization of the release of entrapped lipase [30]. The observed increase in speciﬁc activity with increasing...

Báo cáo khóa học: Endoplasmic reticulum-associated degradation of glycoproteins bearing Man5GlcNAc2 and Man9GlcNAc2 species in the MI8-5 CHO cell line docx

... lgÆmL)1castanospermine, an inhibitor of glucosidases I and II. In the presence of castanospermine, the levels of both Glc1Man9GlcNAc2 and peak X were increased(Fig. 2A,B). Under these conditions, the decreasingamounts ... 2003Most of the evidence for the occurrence of degradationsignals involved in ERAD has been obtained by studying the effect of inhibitors of demannosylation (kifunensine and deoxymannojirimycine) ... min of preincubation in 0.175 mMGlc (T0 h) and chased in the culture medium containing 5 mMGlc and 5 mMManfor 3 and 6 h (T3 h and T6 h, respectively). The cells were then submitted to the...

Báo cáo khoa học: Purple membrane lipid control of bacteriorhodopsin conformational ﬂexibility and photocycle activity An infrared spectroscopic study docx

... under the loop region containing the charged acidic amino acidsshould produce a strain limiting the mobility of both the amino-acid-containing loops and the attached transmem-brane a-helices. These ... reconstitution in lipid dispersions containing vari-ous concentrations of NaCl which control the extent of lipidrebinding. The intrinsic mobility of the transmembranea-helices of bacteriorhodopsin in ... intensity and bandwidth of the amide IImode on the release of retinal induced by either heat or light(S. Barnett & I. W. Levin, unpublished work). These smalldecreases in the amide I and amide...

Báo cáo khoa học: Discriminating between the activities of human cathepsin G and chymase using ﬂuorogenic substrates potx

... Speciﬁcinhibition of thrombin-induced cell activation by the neutrophil proteinases elastase, cathepsin G, and pro-teinase 3: evidence for distinct cleavage sites within the aminoterminal domain of ... speciﬁcity of CG and chymase The crystallographic data reported by Hof et al. [1]indicate that the side-chain of Arg41 located on the 30S insertion loop in CG projects from the molecularsurface to the ... Ribbon plot of CG and chymase in irreversible complexes with syn-thetic inhibitors showing ball -and- stick mod-els for the seven residues located in the vicinity of the active site. The molecular...

Tài liệu Báo cáo khoa học: Autophagy inhibits reactive oxygen species-mediated apoptosis via activating p38-nuclear factor-kappa B survival pathways in oridonin-treated murine ﬁbrosarcoma L929 cells doc

... by 3-methy-ladenine or small interfering RNA against LC3 and Beclin 1 promotedoridonin-induced cell apoptosis. The effects of p38 and nuclear factor- kappa B in oridonin-induced apoptosis and ... Asshown in Fig. 9B, the inhibition of autophagyincreased the oridonin-induced SubG1cell proportion in L929 cells. Furthermore, we knocked down the expressions of Beclin 1 and LC3 by using speciﬁcsiRNAs. ... involved in the activation of ERK pathway in oridonin-induced L929cells.Inhibition of NF-jB and p38 activations increasesoridonin-induced apoptosis and ROSaccumulation The transcription factor...

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