Báo cáo khoa học: K182G substitution in DevR or C8G mutation in the Dev box impairs protein–DNA interaction and abrogates DevR-mediated gene induction in Mycobacterium tuberculosis doc

... K182G substitution in DevR or C8G mutation in the Dev box impairs protein–DNA interaction and abrogates DevR- mediated gene induction in Mycobacterium tuberculosis Rajesh ... residue in DevR func-tion was assessed in vitro and in vivo using DevR K182G or K182A mutant protein and fdxA promoterharbouring mutations in C8 or ⁄ and T9base in the pri-mary DevR binding ... (phosphorylated cytoplasmic domain ofDevS). Lane 1, DevSP; lane 2, DevSP and DevR K182G mutantprotein; and lane 3, DevSP and WT DevR protein. The top panelrepresents the phosphorimage and the...

Báo cáo khoa học: Distinguishing between different pathways of bilayer disruption by the related antimicrobial peptides cecropin B, B1 and B3 pptx

... bacteria,including the lantibiotics nisin and epidermin and the bacteriocin pediocin PA-1, are most effective against speciﬁctarget organisms and utilize docking molecules present in the cell ... 2), an increase in relative responsewas observed, indicating a time-dependent increase in mass and suggesting that the peptides were interacting intimatelyFig. 1. Diagram detailing the liposome ... including cecropins, magainins and melittin or melittin-cecropin chimaeras, have shown that these peptidesdo not function via enzymatic processing or a chiral-speciﬁcreceptor, but by formation...

Báo cáo khoa học: Structure–activity relation for synthetic phenoxazone drugs Evidence for a direct correlation between DNA binding and pro-apoptotic activity pdf

... existence of a terminaldimethylamino group in the alkylamino side chain is animportant factor for anti-tumour activity. Minor modiﬁca-tions in the dimethylaminoalkyl side chain (e.g. elongationby ... possibleanchoring mechanisms and their different activities withnucleic acids [4]. These drugs include intercalators, groovebinders, and those binding with a combination of the twomechanisms. The ... parameters,DHbind, DSbind and DGbind, are summarized in Table 2.Differences in interaction of ActII–ActV with DNA canalso be estimated using the binding parameters DHbind,Fig. 2. Dose-dependent induction...

Báo cáo khoa học: "Medical emergency teams: deciphering clues to crises in hospitals"

... systematically ignore (and find) patientdeterioration. Hospitals in the future must develop methodologiesto find more reliably patients who are in crisis, and then respond tothem swiftly and effectively ... organizations such as the Institute forHealthcare Improvement and the Society for Critical CareMedicine have been promoting rapid response teams and METs. In North America and in Europe, there now appears ... of the METpatient; for example, who is at risk, and what conditions and settings are dangerous? In other words, we do notunderstand the epidemiology of the MET patient. It ispossible that there...

Tài liệu Báo cáo khoa học: Modulation of a-synuclein aggregation by dopamine in the presence of MPTP and its metabolite pptx

... 1B shows the patternseen after the development of the blot. With increase in time of incubation, the intensity of the band for the monomeric protein decreased, whereas the bands for the higher ... 0.096 and 0.177 h)1fora-synuclein incubated alone, and in the presence of100 lm MPTP and 200 lm MPTP, respectively. Nota-bly, in the presence of the neurotoxin, there was adelay in the lag ... for the ﬁbrillation of a-synuclein. For this,a-synuclein was incubated in the presence of MPTP and MPP+along withdopamine. The ﬁbrillation of a-synuclein was monitored by Thioﬂavin Tﬂuorescence...

Tài liệu Báo cáo khoa học: Animal models of amyloid-b-related pathologies in Alzheimer’s disease docx

... matching them for gen-der, and having sufﬁcient power are thereforeextremely important in minimizing the in uence ofconfounding factors arising from inherent problemsassociated with breeding APP ... identiﬁed AD mutation was located in the APP gene [3], although the majority of mutations were caused by geneticlesions in the presenilin (PS) genes, PS1 and PS2. The mutations either enhance the steady-state ... or expressing normal or mutant genes and examiningAPP ⁄ Ab-related phenotypes. A major caveat wheninterpreting the results from a cross-breeding experi-ment is the in uence of the genetic background....

Tài liệu Báo cáo khoa học: Neuronal growth-inhibitory factor (metallothionein-3): evaluation of the biological function of growth-inhibitory factor in the injured and neurodegenerative brain pdf

... of a brain-speciﬁc MT isoform, GIF, in 1991, sparked considerable interest in understanding the role of all MTs in the brain, and particularlywithin the injured or diseased brain. In the case ... properties. In this review, we examine the recent developments regarding the function of neuronal growth-inhibi-tory factor within the brain, particularly in response to brain injury or during neurodegenerative ... suggesting that this MT isoform has different and distinct functions within the brain. Furthermore, the discovery and continued investigation of this brain-spe-ciﬁc MT isoform has led to intense interest...

Tài liệu Báo cáo khoa học: Plasticity of laccase generated by homeologous recombination in yeast docx

... D1 and D2), acquired a T2–T3 clusterbinding site (the dioxygen reduction site mapping at the boundary of D1 and D3) and substrate-bindingsites (one for the electron donor and one for O2 )in neo-formed ... Cold Spring HarborLaboratory Press, Cold Spring Harbor, New York.37 Bradford M (1976) A rapid and sensitive method for the quantitation of microgram quantities of proteinutilizing the principle ... is used for the hybrids; the surface of the parts of hybrids originating from LAC3 is coloured in light blue; the surface of the parts of hybrids originating from eitherLAC1, LAC2 or LAC5 but...

Tài liệu Báo cáo khoa học: Collagen I regulates matrix metalloproteinase-2 activation in osteosarcoma cells independent of S100A4 pdf

... secreted as inactive proenzymes and latency is maintained by an interaction between acystein residue in the prodomain and Zn2+ in the active site of the catalytic domain. Two major types ofendogenous ... cell linesresulted in a reduced in vitro and in vivo invasive and metastatic capacity [12,13]. Furthermore, we alsoshowed that the reduction in the endogenous level ofS100A4 in these cell lines ... either in or close to the autoactivation site. Norcould the activation be explained by autoactivationcaused by a direct binding of either the 72 kDa pro-form or the 64 kDa intermediate to the...

Tài liệu Báo cáo khoa học: Characterization of 1H NMR detectable mobile lipids in cells from human adenocarcinomas doc

... prepared from the same cellculture were used to assess the error on the single mea-surement. Fitting of data to obtain model parameters wasperformed using origin software (OriginLab Corp.,Northampton, ... PCAextracts and are reported in Table 2. The standarddeviation was 0.005 p.p.m. for chemical shift values and 10% for linewidths and IR.Signals from the headgroups of glycerophosphoryl-choline (GPC) ... metabolism,including PL synthesis, slows down accordingly. On the other hand, intensities of ML (in cells) signals,mainly due to TG according to a previous study [13]as well as the present...

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