Báo cáo khoa học: K182G substitution in DevR or C8G mutation in the Dev box impairs protein–DNA interaction and abrogates DevR-mediated gene induction in Mycobacterium tuberculosis doc

... K182G substitution in DevR or C 8 G mutation in the Dev box impairs protein–DNA interaction and abrogates DevR- mediated gene induction in Mycobacterium tuberculosis Rajesh ... residue in DevR func- tion was assessed in vitro and in vivo using DevR K182G or K182A mutant protein and fdxA promoter harbouring mutations in C 8 or ⁄ a...

Ngày tải lên: 22/03/2014, 16:20

... bacteria, including the lantibiotics nisin and epidermin and the bacteriocin pediocin PA-1, are most effective against specific target organisms and utilize docking molecules present in the cell ... 2), an increase in relative response was observed, indicating a time-dependent increase in mass and suggesting that the peptides were interacting intimately Fig. 1. Diagram de...

Ngày tải lên: 08/03/2014, 08:20

... existence of a terminal dimethylamino group in the alkylamino side chain is an important factor for anti-tumour activity. Minor modifica- tions in the dimethylaminoalkyl side chain (e.g. elongation by ... possible anchoring mechanisms and their different activities with nucleic acids [4]. These drugs include intercalators, groove binders, and those binding with a combination of th...

Ngày tải lên: 31/03/2014, 07:20

... systematically ignore (and find) patient deterioration. Hospitals in the future must develop methodologies to find more reliably patients who are in crisis, and then respond to them swiftly and effectively ... organizations such as the Institute for Healthcare Improvement and the Society for Critical Care Medicine have been promoting rapid response teams and METs. In Nort...

Ngày tải lên: 25/10/2012, 10:45

... 1B shows the pattern seen after the development of the blot. With increase in time of incubation, the intensity of the band for the monomeric protein decreased, whereas the bands for the higher ... 0.096 and 0.177 h )1 for a-synuclein incubated alone, and in the presence of 100 lm MPTP and 200 lm MPTP, respectively. Nota- bly, in the presence of the neurotoxi...

Ngày tải lên: 14/02/2014, 19:20

... matching them for gen- der, and having sufficient power are therefore extremely important in minimizing the in uence of confounding factors arising from inherent problems associated with breeding APP ... identified AD mutation was located in the APP gene [3], although the majority of mutations were caused by genetic lesions in the presenilin (PS) genes, PS1 and PS2. The...

Ngày tải lên: 16/02/2014, 09:20

... of a brain-specific MT isoform, GIF, in 1991, sparked considerable interest in understanding the role of all MTs in the brain, and particularly within the injured or diseased brain. In the case ... properties. In this review, we examine the recent developments regarding the function of neuronal growth-inhibi- tory factor within the brain, particularly in response...

Ngày tải lên: 16/02/2014, 15:20

... D1 and D2), acquired a T2–T3 cluster binding site (the dioxygen reduction site mapping at the boundary of D1 and D3) and substrate-binding sites (one for the electron donor and one for O 2 )in neo-formed ... Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. 37 Bradford M (1976) A rapid and sensitive method for the quantitation of microgram quantities of pro...

Ngày tải lên: 18/02/2014, 06:20

... secreted as inactive proenzymes and latency is maintained by an interaction between a cystein residue in the prodomain and Zn 2+ in the active site of the catalytic domain. Two major types of endogenous ... cell lines resulted in a reduced in vitro and in vivo invasive and metastatic capacity [12,13]. Furthermore, we also showed that the reduction in the endoge...

Ngày tải lên: 18/02/2014, 11:20

... prepared from the same cell culture were used to assess the error on the single mea- surement. Fitting of data to obtain model parameters was performed using origin software (OriginLab Corp., Northampton, ... PCA extracts and are reported in Table 2. The standard deviation was 0.005 p.p.m. for chemical shift values and 10% for linewidths and IR. Signals from the headgroups o...

Ngày tải lên: 18/02/2014, 13:20