Báo cáo khoa học: Molecular structures and functional relationships in clostridial neurotoxins potx

... the single chain molecule. The position of the binding domain of E could be obtained by rotating the binding domain of A or B about the lin- ker region connecting the translocation and binding domains. ... B there are only limited interactions between the translo- cation and binding domains and no interaction between the binding and catalytic domains. In BoNT ⁄ E, all three domains...

Ngày tải lên: 22/03/2014, 15:21

... and 80 °C between 190 and 260 nm with a step increase of 0.2 nm, and a bandwidth of 1 nm. Thermal stability of Rep245 was measured by incu- bating the protein at 60, 70 and 80 °C for 30 min and ... self-standing structure protruding from the interface between the DNA binding and the active site subdomains, we man- aged to model the entire domain without it. Another significant findi...

Ngày tải lên: 18/02/2014, 18:20

... are indicated by solid thin lines (b 1 ) andshadedthinlines(b 1B ), and the unidenti- fied 3¢ untranslated region of b 1B is indicated using the thin interrupted broken line. The stop codon is indicated ... UÆmL )1 ;Gibco)inCa 2+ -free medium, followed by rinsing and storage in saline medium containing Ca 2+ and 50 lgÆmL )1 gentamicin. Stage V–VI oocytes were separated for injection th...

Ngày tải lên: 16/03/2014, 23:20

... administration. The lead II ECG maintained continuous readings using a polygraph system. One hour after the intravenous admini- stration of BmKIM (dissolved in distilled water and administered in ... lepidopterous and dipterous insects [8]. The excitatory toxins cause a fast excitatory paralysis in animals and induce repetitive firing in insect nerves; in contrast, the depressa...

Ngày tải lên: 31/03/2014, 09:20

... in the eyestalk but not in muscle or stomach. We also inves- tigated the ability of dsRNA to inhibit the CHH function in shrimps in vivo. Injection of CHH dsRNA into the abdominal hemolymh sinuses resulted ... to examine in this research the CHH gene expression in the stomach, including the fore gut site. Recently, CHH was reported in the endocrine cells of the fore gut and hi...

Ngày tải lên: 23/03/2014, 10:20

... proteolytic processing), and GmPDIL-1 and GmPDIL-2 associate with proglycinin and b-con- glycinin in the ER, suggesting that they may play important roles in folding and in formation and rearrangement ... redox-active CGHC motifs within the a-domain and a¢-domain. Instead, they both contain the sequence CPRS in the a-domain and CMNC or CINC in the a¢-domain. GmPDIL-3a...

Ngày tải lên: 18/02/2014, 11:20

... protein was N-terminally blocked. Besides immunoblotting we also performed a Covalink- ELISA to determine IgE binding to the natural Lyc e 2 and the recombinant protein. All sera reacting with the ... contaminating 90-kDa band was also inhibited by MUXF, so this protein may be an IgE reactive glycoprotein as well. Preincubation of a non- CCD binding serum with MUXF had no effect on the IgE-b...

Ngày tải lên: 21/02/2014, 00:20

... serine protease inhibitor 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF) (Fig. 2B). However, two other serine protease inhibi- tors, aprotinin and antipain, had no effect, although the latter interferes ... l M), pepstatin A (100 lM), AEBSF (1 mM), aprotinin (0.3 l M), antipain (75 lM) and a mixture containing all inhibitors in the assigned concentrations. Scpep1 was immunopre- cipitat...

Ngày tải lên: 07/03/2014, 03:20

... that disrupt the folding of glycinin or b-conglycinin, suggesting that GmPDIL-1 and GmPDIL-2 are involved in the proper folding or quality control of such storage proteins as molecular chaperones. Abbreviations AZC, L-azetidine-2-carboxylic ... suggested that GmPDIL-1 and GmPDIL-2 associate with pro- glycinin, a precursor of the seed storage protein glycinin, and the a¢-subunit of b...

Ngày tải lên: 07/03/2014, 05:20

... alkaloid biosyn- thesis in E. californica, because CYP719A2 and CYP719A3 produced (S)-nandinine from (S)-scoulerine in vitro; indeed, CYP719A3 converted (S)-scoulerine to (S)-nandinine with high affinity ... (S)-stylopine is synthesized via (S)-cheilanthifoline, not (S)-nandinine [7,30,31]. If we assume that (S)-nandinine is produced in E. californica, there may be no need for a cataly...

Ngày tải lên: 07/03/2014, 10:20