Mechanism of Eukaryotic Homologous Recombination ppt
Tài liệu ECCENTRIC MECHANISM OF FOUR-BAR LINKAGE pptx
... select the surface of eccentric, then select the front surface of the bushing as shown below. 28 Pick this surface of eccentric part. Then pick the front surface of the bushing. ... the name of arm_asm. OK. 21 Click . Then open arm.prt and assemble it at default location by selecting . Open clip-1.prt and place it in the groove of the pin...
Ngày tải lên: 19/01/2014, 05:20
Báo cáo Y học: Role of conserved residues within helices IV and VIII of the oxaloacetate decarboxylase b subunit in the energy coupling mechanism of the Na+ pump ppt
... Role of conserved residues within helices IV and VIII of the oxaloacetate decarboxylase b subunit in the energy coupling mechanism of the Na + pump Markus Schmid, Thomas ... membrane-bound b subunit of the oxaloacetate decarboxylase Na + pump of Klebsiella pneumoniae catalyzes the decarboxylation of enzyme-bound biotin. This event is coupled to the transport of 2 Na + ions ......
Ngày tải lên: 08/03/2014, 23:20
Báo cáo khoa học: The role of Tyr71 in Streptomyces trypsin on the recognition mechanism of structural protein substrates ppt
... sche- matically. The recombination sites of chimeras A–H are shown in (B). The primary structures of SOT (upper) and SGT (lower) are shown. The recombination sites of the chimeras are boxed. The names of the ... isothiocyanate (FITC). One unit of activity was defined as the amount of the enzyme necessary to release 1 nmol of FITC per minute under these assay conditions. The c...
Ngày tải lên: 16/03/2014, 00:20
Tài liệu Báo cáo khoa học: Modeling of tRNA-assisted mechanism of Arg activation based on a structure of Arg-tRNA synthetase, tRNA, and an ATP analog (ANP) ppt
... tRNA Arg ICG . Distances between C1¢ of C72 of the 1Æ72 pair in the acceptor stem, C1¢ of G18 forming a hydrogen bond to U55 of the T-loop and C1¢ of C31 or G39 of 31Æ39 of the anticodon stem of tRNA were compared ... formation of the hydrogen bond between O2 of the carboxyl group of the cognate amino acid and the 2¢-OH group of the ribose moiety of A76 maintains the...
Ngày tải lên: 18/02/2014, 11:20
Tài liệu Báo cáo khoa học: Manipulation of prenyl chain length determination mechanism of cis-prenyltransferases ppt
... parts of sequences of the mutants of M. luteus B-P 26 UPP synthase (EKE, RAKDY) and of Srt1p (Srt1p-delta) are shown. (B) TLC autoradiograms of polypre- nyl alcohols corresponding to products of cis-prenyl ... described [29]. In order to understand the molecular mechanisms of chain length determination, we built structural models of Rv1086 and of LLLH mutant of M. luteus U...
Ngày tải lên: 19/02/2014, 07:20
Tài liệu Báo cáo Y học: Insights into the reaction mechanism of Escherichia coli agmatinase by site-directed mutagenesis and molecular modelling ppt
... present findings of a markedly decreased activity of a D153N variant of E. coli agmatinase. From the enzymic properties of D153N agmatinase and a modelled structure, we conclude that the lower activity of ... extension, in the case of E. coli agmatinase, of the loop indicated by the letter a. Fig. 5. Scheme of the binuclear manganese cluster and the localization of the side-c...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: Dynamic mechanism of nick recognition by DNA ligase ppt
... 5¢-phosphate of the nick and backwards in the 5¢-direction of the nicked strand. Fig. 3. Illustration of the proposed mechanism of dynamic nick recognition by DNA ligase. (Left) binding of the dsDNA. ... [35,37,41]. Nonspecific interactions lead to a decrease of the degree of hydration of the bound DNA. As a Fig. 2. Structures of DNA polymerase domain of HIV-1 reverse tra...
Ngày tải lên: 21/02/2014, 01:21
Báo cáo khoa học: The product chain length determination mechanism of type II geranylgeranyl diphosphate synthase requires subunit interaction pptx
... volume of 200 lL, 0.5 nmol of [1- 14 C]IPP (2 GBqÆmmol )1 ), 0.5 nmol of an allylic primer (GPP, FPP or GGPP), 1 lmol of MgCl 2 ,2lmol of a Tris– HCl buffer (pH 8.0) and a suitable amount of each ... determination mechanism. This conclusion is supported by the recently-solved crystal structure of type II GGPS from mustard [23]. The mechanism of product chain length determin...
Ngày tải lên: 07/03/2014, 06:20
Báo cáo khoa học: Branched chain mechanism of polymerization and ultrastructure of prion protein amyloid fibrils ppt
... important role in the evolution of our ideas regarding the mechanism of prion conversion, neither of them emphasize the importance of multiplication of the active centers of prion conversion, a key ... FEBS consistent with the mechanism of branched-chain reactions. Employing the theory of branched-chain reactions will greatly benefit our understanding of the prion rep- li...
Ngày tải lên: 07/03/2014, 10:20
Báo cáo khóa học: Insight into the activation mechanism of Bordetella pertussis adenylate cyclase by calmodulin using fluorescence spectroscopy pptx
... direct exploitation of the structural results of the former to explain the mechanism of CaM activation of the latter. Crystallization and resolution of the 3D structures of AC and AC–CaM, which ... the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254. 36. Laemmli, U.K. (1970) Cleavage of structura...
Ngày tải lên: 07/03/2014, 15:20