... flow rate of 0.5 mLÆmin)1with a 20-min linear gradient (5–30 mM )of sodium phosphate (pH 7.5), in 20 mMtetrabutylam-monium bromide/20%methanol (v/v) (buffer A) followed by a 10-min linear gradient ... the5-min incubation are shown in Fig. 3. No appreciable synthesis of poly(A) (spot at the origin) was observed in the absence of Gp2G, whereas in its presence the ATPspot decreased, increasing ... preferences: comparing dinucleotideswith the same number of inner phosphates, guaninedinucleotides are more active than adenine dinucleotidesand, adenine dinucleotides with even number of innerphosphates...