GENE CLONING AND DNA ANALYSIS pptx

... Principles of Gene Cloning and DNA Analysis 1 | Why Gene Cloning and DNA Analysis are Important 3 2 | Vectors for Gene Cloning: Plasmids and Bacteriophages 13 3 | Purification of DNA from Living ... gene 258 14.3 Gene therapy 259 14.3.1 Gene therapy for inherited diseases 259 14.3.2 Gene therapy and cancer 260 14.3.3 The ethical issues raised by gene the...

Ngày tải lên: 19/03/2014, 09:20

... Principles of Gene Cloning and DNA Analysis 1 | Why Gene Cloning and DNA Analysis are Important 3 2 | Vectors for Gene Cloning: Plasmids and Bacteriophages 13 3 | Purification of DNA from Living ... gene 258 14.3 Gene therapy 259 14.3.1 Gene therapy for inherited diseases 259 14.3.2 Gene therapy and cancer 260 14.3.3 The ethical issues raised by gene the...

Ngày tải lên: 06/03/2014, 22:20

... 5¢-upstream region was cloned and analyzed. Transient analysis revealed that the Pokemon promoter is constitutive. Deletion analysis and a DNA decoy assay indicated that the NEG-U and NEG-D elements were ... sup- pressor gene and is a central regulator in oncogenesis. Overexpression of the Pokemon gene can decrease expression of the ARF gene, which in turn results in p53 de...

Ngày tải lên: 30/03/2014, 04:20

... the Analysis • Sensitivity and Breakeven analyses are also known as: ‘scenario analysis , and ‘what-if analysis . • Point values of forecasts are known as: ‘optimistic’, ‘most likely’, and ... abandoned or sold off prior to its planned termination. Using Sensitivity: Sensitive variables are investigated and managed in two ways: 6 Management Use of Sensitivity and Breakeven...

Ngày tải lên: 16/03/2014, 01:20

... facilities of the GeneDB resource (http://www.genedb. org) [66] which unites nucleic acid and protein sequence information obtained from the T. brucei and L. major genome projects and a separate ... positive clones were processed, the DNA was purified and appropri- ate restriction fragments containing the PFK-2 and FBPase-2 genes were subcloned in plasmid vectors. Each part of the...

Ngày tải lên: 16/03/2014, 22:20

Ngày tải lên: 23/03/2014, 14:20

... Matrices and Multiplier Analysis Activities and commodities The SAM distinguishes between “activities” and ”commodi- ties.” Activities are the entities that produce goods and services, and commodities ... the SAM, total demand Z in each sector is the sum of intermediate input demand, household consumption demand, and other exogenous sources of demand E, such as public consumpti...

Ngày tải lên: 29/03/2014, 20:20

... thermophilus through random PCR mutagenesis Gene cloning and protein characterization Anna LoÈnn 1 ,Ma  rk Ga  rdonyi 1 , Willem van Zyl 2 ,BaÈ rbel Hahn-HaÈ gerdal 1 and Ricardo Cordero Otero 2, * 1 Department ... F163L (M-1024) and E372G (M-1026). The wild- type and mutated xylA genes w ere cloned and expressed in Escherichia coli HB101 using the v ector pGEM Ò-T Easy, and...

Ngày tải lên: 31/03/2014, 15:20

... sand and then transfer the hot sand to the pyrolysis vessel. Such an option has advantages but also is more challenging because of solids transport and temperature control (overheating of sand ... movement of sand and particles through the system causes abrasion of this char layer but mostly at the elbows and bends where there is more forceful interaction between the particles an...

Ngày tải lên: 01/04/2014, 00:20

... rerio ) cDNA sequence, tissue-specific expression and gene linkage analysis Marianne C. Cameron 1 , Eileen M. Denovan-Wright 2 , Mukesh K. Sharma 1 and Jonathan M. Wright 1 1 Department of Biology, and 2 Department ... cDNA probe used for Northern blot and radiation hybrid linkage mapping analysis are boxed and numbered Ô1Õ and Ô2Õ, respectively. The polyadenylation signal se...

Ngày tải lên: 17/03/2014, 11:20