gene protein level features and correlation analysis

... non-essential genes and was used directly from the Levy and Siegal study [38] Materials and methods Multi-functionality Description of gene/ protein level features and correlation analysis This ... JB, CM and PK conceived the project JB, SH, MM and TK designed and implemented the analysis JB, SH, MM, MC, BA, CB, GB, CM and PK wrote the paper CM and PK helped to develop the approach and supervised ... disordered residues of yeast proteins by their number of GIs (b) Multi-functionality (see Materials and methods) for disordered and structured GI hubs and non-hubs Hubs are genes in the top 90th percentile...

Ngày tải lên: 09/08/2014, 22:23

... LL-LL-LL band UWPT: LL-LL-LL band UWPT: LL-LL-LH band UWPT: LL-LL-HL band UWPT: LL-LL-HH band (b) Figure 1: (a) Undecimated wavelet packet transform tree for one-dimensional signal x, where A stands ... mirror filter (QMF) for creating the subbands is M, and the number of decomposition levels is L such that L ≤ log N NM and NA represent “number of multiplications” and “number of additions” that are ... of the head, and partial occlusion The WT and color histogram-based algorithms (CHC and CHB) have mistakenly tracked a wrong object in frame nos 139 and 160 (see Figures 6(c), 6(d), and 6(e)) As...

Ngày tải lên: 22/06/2014, 19:20

... plants (shrubs, trees) [20] As genetic selection and breeding activities in trees are being expanded to include genetic mapping and molecular markers, candidate genes like HD-Zip III are considered ... spp.) and the gymnosperms (P glauca) Distinct HD-Zip III gene family evolution in gymnosperm and angiosperm trees Gene sequences isolated from the moss P patens with features typical of HD-Zip genes ... reaction and with the transcript level of a reference gene The spruce data were normalised to the reference of transcript level gene encoding Elongation factor alpha, EF1a, (AJ132534) [56] The EF1a gene...

Ngày tải lên: 11/08/2014, 11:21

... performance analysis, including research model, data analysis, discussion and implications Section presents the firm productivity analysis, including research model, data analysis, discussion and implications ... build the balance sheet model, and build the income statement model with cost of goods sold, expenses on research and development (R&D), and expenses on selling, general and administrative activities ... performance of SaaS firms We present the performance analysis and productivity analysis separately in Section and Section In performance analysis, we look into whether the business model of a...

Ngày tải lên: 06/10/2015, 20:57

... Applications of Gene Cloning and DNA Analysis in Biotechnology 223 13 14 15 16 Production of Protein from Cloned Genes 225 Gene Cloning and DNA Analysis in Medicine 245 Gene Cloning and DNA Analysis ... Principles of Gene Cloning and DNA Analysis 1 Why Gene Cloning and DNA Analysis are Important 1.1 1.2 1.3 1.4 1.5 1.6 The early development of genetics The advent of gene cloning and the polymerase ... Edition xvi Part I The Basic Principles of Gene Cloning and DNA Analysis 1 Why Gene Cloning and DNA Analysis are Important Vectors for Gene Cloning: Plasmids and Bacteriophages 13 Purification of DNA...

Ngày tải lên: 06/03/2014, 22:20

... envelope between 11 and 26 charges (m ⁄ z 4982–2108) was observed, and for hexamers and nonamers, complete envelopes between 20 and 35 charges (m ⁄ z 5480–3132) and between 28 and 37 charges (m ... laevis surfactant protein C and B genes Gene Expr Patterns 7, 8–14 Nogee LM, Dunbar AE III, Wert S, Askin F, Hamvas A & Whitsett JA (2002) Mutations in the surfactant protein C gene associated ... 1.2 and 1.9 kV, and cone and RF lens potentials were 100 and 38 V, respectively The pumping of the ESI interface region was restricted; backing pirani vacuum gauge from 1.8 to 1.95 mbar, and...

Ngày tải lên: 07/03/2014, 05:20

... (1995) The human glioma pathogenesis-related protein is structurally related to plant pathogenesis-related proteins and its gene is expressed specifically in brain tumors Gene 159, 131–135 42 Chow ... Proteolytic treatment and amino acid sequence analysis Cyn d 24 was first denatured and reduced with 2-mercaptoethanol and the reduced protein was digested overnight at 37 °C with endoproteinase Lysyl-C ... program, and multiple amino acid sequence alignments were performed using the genedoc program A newly identified pollen allergen as pathogenesis-related proteins Glycopeptide identification and analysis...

Ngày tải lên: 07/03/2014, 12:20

... homologue gene is coded far away, after the gene HP1286, corresponding to an YceI protein homologue, defining a divergon with the downstream genes HP1290 and HP1291 [4] Recently, a transposon mutagenesis ... apparently lacks the genes devoted to the biosynthesis of the thiamin precursor HMP and HET moieties Moreover, the two genes HP1290 and HP1291 could define a divergon with the gene coding for the ... Gasteiger E, Hoogland C, Gattiker A, Duvaud S, Wilkins MR, Appel RD & Bairoch A (2005) Protein Identification and Analysis Tools on the ExPASy Server In The Proteomics Protocols Handbook (Walker...

Ngày tải lên: 16/03/2014, 00:20

... a-helices and 12% b-turns (Fig 1) Incubation of the protein with different ligands such as palmitate, caprylic and arachidonic acids and retinol in concentrations sufficient to saturate the protein ... helminth lipidbinding proteins offload their ligands to membranes: differential mechanisms of fatty acid transfer by the ABA-1 polyprotein allergen and Ov-FAR-1 proteins of nematodes and Sj-FABPc of ... subtraction, normalization and smoothing were performed by using jasco cd j-715 data manipulation software and the analysis of the data was carried out with the programs contin and selcon Protein labeling...

Ngày tải lên: 16/03/2014, 18:20

... CCTTCGATGTCGGCTCT-3¢) and 28S-R (5¢-CTAA CCTGTCTCACGACGGT-3¢), and was used as an internal control probe in Northern hybridization for normalization of gcGLUT expression Phylogenetic analysis Phylogenetic analysis ... GLUT1 and GLUT3 (exons 3–8), and four in human GLUT2 (exons 7–10) and GLUT4 (exons 6–9) (Table 1) The codons for arginine (96) and valine (231) in gcGLUT (Fig 2) are split between exons and 5, and ... the gcGLUT gene (A) The 12 exons of the gcGLUT gene are shown as boxes Filled and open boxes indicate translated and untranslated regions, respectively The position of the start (ATG) and stop...

Ngày tải lên: 17/03/2014, 03:20

... band of 28 kDa under reducing conditions and two bands of 33 and 40 kDa under nonreducing conditions 2-ME, 2-mercaptoethanol (B) The N-terminal amino-acid sequences of three bands The 28-kDa protein ... fusion proteins (A) GST fusion proteins including L1, L2, and L1(DL2) (B) The fusion proteins were expressed in clones generated by PCR from the spermosin cDNA as described in Materials and methods ... designated L1), and the 33-kDa protein consisted of the heavy chain (residues 130±388) and a light chain designated as L2 (residues 97±129) There were no distinct bands of L1 and L2 chains on...

Ngày tải lên: 17/03/2014, 11:20

... Applications of Gene Cloning and DNA Analysis in Biotechnology 223 13 14 15 16 Production of Protein from Cloned Genes 225 Gene Cloning and DNA Analysis in Medicine 245 Gene Cloning and DNA Analysis ... Principles of Gene Cloning and DNA Analysis 1 Why Gene Cloning and DNA Analysis are Important 1.1 1.2 1.3 1.4 1.5 1.6 The early development of genetics The advent of gene cloning and the polymerase ... Edition xvi Part I The Basic Principles of Gene Cloning and DNA Analysis 1 Why Gene Cloning and DNA Analysis are Important Vectors for Gene Cloning: Plasmids and Bacteriophages 13 Purification of DNA...

Ngày tải lên: 19/03/2014, 09:20

... of WT and K182G mutant DevR proteins with DevS201P (phosphorylated cytoplasmic domain of DevS) Lane 1, DevSP; lane 2, DevSP and DevR K182G mutant protein; and lane 3, DevSP and WT DevR protein ... 0.7, 0.8, 0.9 lM of WT DevR protein; lane 10 represents free DNA; lanes 11 and 12 contain 0.5 and 1.0 lM of K182G mutant DevR protein (C) qRT-PCR analysis of DevR regulon gene expression RNA was ... O6 and N7 atoms of G13 and with the N7 atom of A12 in the DNA strand In silico analysis of WT versus K182 mutant DevR protein reveals the loss of three K182mediated H bonds in the mutant protein...

Ngày tải lên: 22/03/2014, 16:20

... RNase III proteins, Drosha and Dicer, collaborate in the stepwise processing of micro RNAs (miRNAs), and play key roles in miRNA-mediated gene regulation of processes such as development and differentiation ... of these have been purified and characterized as alkaline [12] and acidic RNases [13], and a third poly(U)- and poly(C)-specific ribonuclease has also been isolated and characterized [14,15] In ... Rampias et al Genomic structure and expression analysis of the Cc RNase epidermis and intestine of 6-day-old larvae and adult heads and ovaries, was blotted and hybridized using the Cc RNase cDNA as...

Ngày tải lên: 23/03/2014, 06:20

... of the protein Nevertheless, different protein preparations showed the same specificity and affinity when considering the amount of bound protein as the sum of both shifted bands These bands displayed ... (A) and I50K–HAKN1 (B) bound to their preferred binding sites After binding and hydroxyl radical attack, free (F) and bound (B) DNA were separated and analysed The left and right panels in (A) and ... Ile50 and Arg55 must have been particularly important Other homeodomains that contain Ile50 and Arg55 are those of the TGIF, Meis and Bell families [22] and yeast MATa1 [36] TGIF and Meis proteins...

Ngày tải lên: 23/03/2014, 13:20

... digital camera (Princeton Instrument) and stored on a CD for subsequent analysis Estimations of Glu- and Tyr-microtubules present in fields selected at random were obtained by measuring the integrated ... the assembled and nonassembled tubulin fractions from cells treated and nontreated with lM OA was concentrated on phosphocellulose columns (for details see Materials and methods) and enzyme activity ... microtubule-stabilizing buffer, and homogenized to isolate the microtubular and soluble tubulin fractions Both fractions were concentrated and assayed as described in Materials and methods Carboxypeptidase...

Ngày tải lên: 23/03/2014, 15:21

... activity of KCTD10; and SP1 and AP-2a can regulate the promoter activity and endogenous KCTD10 expression oppositely through binding this region SP1 and AP-2a regulate KCTD10 Results Analysis of genomic ... SP1 and AP-2a regulate KCTD10 R Liu et al A B Fig Genomic structure of human and mouse KCTD10 genes, and the 5¢-flanking region sequence of human KCTD10 (A) Genomic organization of human and mouse ... of AP-2a or Sp1 alter the KCTD10 level, we checked endogenous KCTD10 expression in HeLa cells at the mRNA and protein levels using quantitative real-time RT-PCR and western blot through overexpressing...

Ngày tải lên: 30/03/2014, 02:20

... tumor suppressor gene and is a central regulator in oncogenesis Overexpression of the Pokemon gene can decrease expression of the ARF gene, which in turn results in p53 degradation and oncogenic ... Pokemon gene both inhibited oncogene-mediated cellular transformation and induced cell senescence and apoptosis [22,26] Therefore, Pokemon plays a crucial role in cell tumorigenesis and may be ... sequences (254 and 106 bp) between the NEGU and NEG-D elements The distance between the NEG-U and NEG-D elements was 253 and 106 bp in D-254 and D-106, respectively The right-hand panel shows...

Ngày tải lên: 30/03/2014, 04:20

... isolated and subjected to restriction analysis and subcloning Phage k5 harbored the cardosin A gene, and the remaining phages contained two new cardosin genes, designated cardosins C (k6) and D ... this study, the genomic sequences of the cardosin A and B genes and of two new cardosin genes (those encoding cardosins C and D) were isolated and characterized Our results showed that in cardoon ... tropomyosin gene [25] and in the A thaliana phenylalanine ammonia-lyase gene (GenBank accession number X84728) ‘CAAT’ motifs are present in positions ) 71 bp and ) 74 bp of the cardosin A and D genes,...

Ngày tải lên: 30/03/2014, 09:20

... with the procedures generally used m protein chemistry m terms of senstttvtty and acceptance of solvents, detergents, and buffers, They must be able to handle impure samples and complex mixtures ... K L., Glish, G L., and McLuckey, S A (1988) Mass Spectrometry/Mass Spectrometry Technzques and Applications of Tandem Mass Spectrometry VCH, New York 16 Hogeland, K E , Jr and Demzer, M L (1994) ... shown to be capable of the analysis of large underivatized peptides (8), and, soon after, of proteins, by the demonstration of the first mass spectrum of insulin in 1982 (9) and of a number of snake...

Ngày tải lên: 11/04/2014, 10:11