Báo cáo Y học: Cloning and characterization of novel snake venom proteins that block smooth muscle contraction pptx
Báo cáo Y học: Cloning and characterization of novel snake venom proteins that block smooth muscle contraction pptx
... Cloning and characterization of novel snake venom proteins that block smooth muscle contraction Yasuo Yamazaki1, Hisashi Koike1, Yusuke Sugiyama1, Kazuko Motoyoshi1, Taeko ... polylepis).Calciseptineisaknown blocker of L-type Ca2+channels, a property that underlies its ability to block K+-induced contractions of aortic smooth muscle and spontaneous contractions of uterine smooth muscle ... of the artery. Theseresults indicate that several snake venoms contain novel proteins with neurotoxin-like activity.Keywords: snake venom; neurotoxin; helothermine; cysteine-rich secretory...
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Báo cáo Y học: Cloning and characterization of the mammalian-specific nicolin 1 gene (NICN1) encoding a nuclear 24 kDa protein doc
... Hassan Y. Naim2 and Tosso Leeb11Institute of Animal Breeding and Genetics and 2Institute of Physiological Chemistry, School of Veterinary Medicine Hannover;3Institute of Molecular Biology, ... RT-PCRanalysis provided evidence that the murine Nicn1 gene is alsoexpressed during development (Fig. 3). A more detailedanalysis of the expression of the Nicn1 gene at days 8.5, 9.5, and 10.5 of ... 488 nm excitation line of an argon laser and an emission range between 505 and 525 nm forfluorescence detection as described previously [9].RESULTS AND DISCUSSIONAnalysis of the NICN1 cDNA sequenceDuring...
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Tài liệu Báo cáo khoa học: Cloning and characterization of CBL-CIPK signalling components from a legume (Pisum sativum) ppt
... MgCl2without addition of any exogenous Ca2+.Phospho-amino acid analysisThe phosphorylated 26-kDa band of PsCBL and the58-kDa band of PsCIPK were separately eluted from thegel and hydrolysed in 6 n ... 7A, D, I and L are diamidino-2phenylindolehydrochloride (DAPI) stained cells showing the nuc-leus, and Fig. 7C, F, K and N are the merged images of A and B, D and E, I and J, and L and M, respect-ively.DiscussionNature ... response to array of stresses. The exist-ence of a large family of CIPK and CBL genes hasbeen reported in Arabidopsis and rice [15]. However,the details of the role of these proteins and the inter-Stress-induced...
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Tài liệu Báo cáo khóa học: Cloning and characterization of two distinct isoforms of rainbow trout heat shock factor 1 ppt
... formationNobuhiko Ojima and Michiaki YamashitaCell Biology Section, Physiology and Molecular Biology Division, National Research Institute of Fisheries Science,Fisheries Research Agency, Yokohama, JapanTo ... cycle of 15 min at 95 °C,amplification cycles of 0.5 min at 94 °C, 0.5 min at 50 °C and 0.5 min at 72 °C, and one termination cycle of 1 min at72 °C, with 35 cycles in total for HSF1a and HSF1b and ... potentialassemblies of HSF1 trimer, namely, two homotrimers (a3 and b3) and two heterotrimers (a2b1 and a1b2). The existence of the four types of trimer may be reflected in the broadband migrating...
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Tài liệu Báo cáo Y học: Expression and characterization of active site mutants of hevamine, a chitinase from the rubber tree Hevea brasiliensis docx
... activity (Table 3).CONCLUSIONSIn this study we investigated the catalytic role of Asp125,Glu127 and Tyr183 in hevamine by X-ray crystallographic and kinetic analysis of several mutants. We show that Glu127 ... (1995) Stereochemistry of chitinhydrolysis by a plant chitinase/lysozyme and X-ray structure of acomplex with allosamidin: evidence for substrate assisted catalysis.Biochemistry 34, 15619–15623.14. ... refolding and purification they were characterized by both structural and enzyme kinetic studies. Mutation of Tyr183 t o phenylalanineproduced an enzym e with a lower kcat and a slightly higherKmthan...
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Báo cáo khoa học: Cloning and characterization of the genes encoding toxic lectins in mistletoe (Viscum album L) pot
... similarpattern of five bands, one of which was also more intensivethan the others. Thus, the different intensity of the bandsmay be the result of a different copy number of the genes inthe hybridizing ... sequences of the proteins encoded bythe ml1p, ml2p, and ml3p genes are identical to MLI by 98,88, and 77%, respectively. The similarity to MLI of theamino acid sequence encoded by the gene ... singlechain proteins (with few exceptions) resembling the toxinA-chain in structure and function. They are essentiallynontoxic and they are abundantly present in a wide variety of plants. The toxic type...
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Báo cáo Y học: Cloning and expression of sterol D14-reductase from bovine liver potx
... Della Fazia4 and Giuseppe Servillo41Department of Internal Medicine, University of Perugia, Italy;2Department of Pharmacological Sciences, University of Milan,Italy;3Department of Biochemical ... UniversitaÁÔLa SapienzaÕ Roma, Italy;4Department of Biochemical Sciences and Molecular Biotechnology, University of Perugia, ItalyBiosynthesis of cholesterol represents one of the funda-mental cellular ... from y east [7±9] and fungi[10]. Gene c loning of D14-SR from Ara bidopsis thaliana and analysis o f m utants has h ighlighted the role of theprotein in cell growth and embryonic development of...
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Báo cáo Y học: Isolation and characterization of a thioredoxin-dependent peroxidase from Chlamydomonas reinhardtii doc
... by HPLC and some of themwere totally or partially analyzed by Edman sequencing and/ or by MALDI-TOF mass spectrometry ( Fig. 2).Computer database searches based on the amino-acidsequences of ... reductase system in yeast and animals, w hile 1Cys-Prx may be r educed by a smallthiol molecule such as glutathione. R ecently, 1Cys-Prx hasbeen identi®ed i n y east and Arabi dopsis, and has ... residues:the 2Cys-Prx subgroup, and 1Cys-Prx subgroup containtwo and one conserved cysteines, respectively. 2Cys-Prx proteins are reduced by the AhpF protein in bacteria, and by the thioredoxin/thioredoxin...
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Báo cáo Y học: Expression and characterization of recombinant vitamin K-dependent c-glutamyl carboxylase from an invertebrate, Conus textile doc
... carboxylase activity. The effect of proFIX18, proPT18 and pro-e-TxIX/12 on FLEEL carboxylation bythe recombinant bovine carboxylase (closed symbols) and the recom-binant Conus carboxylase (open symbols) ... crude venom duct homogen-ate or bovine carboxylase (Table 3).Effect of the propeptides of vitamin K-dependentprotein precursors onConuscarboxylase activityOur work [43] and that of Bandyopadhyay ... 2002by the addition of proFIX18 and proPT18, indicating that the Conus carboxylase, like the bovine carboxylase, isactivated by the human propeptides.To compare the potency of the effect of these...
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Báo cáo Y học: Cloning and expression of two novel aldo-keto reductases from Digitalis purpurea leaves potx
... Biology (ZMBP), University of Tu¨bingen, Germany;2Department of Plant Biology, University of Valencia, SpainThe aldo-keto reductase (AKR) superfamily comprises proteins that catalyse mainly ... superfamily [8].The members of the AKR superfamily are cytosolic,monomeric proteins that catalyse mainly the NAD(P)H-dependent reduction of a wide variety of carbonyl com-pounds; some enzymes ... molecular phylogenetictree suggests that these seven proteins belong to a new sub-family of the AKR superfamily. Southern analysis indicated that DpARs are encoded by a family of at most five...
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Từ khóa: báo cáo y họcbáo cáo y học cổ truyềnmẫu báo cáo y học cổ truyềnbao cao y hoc colchicinphan ban luan trong bao cao y hoc co truyenstudies on growth crystal structure and characterization of novel organic nicotinium trifluoroaexpression purification and characterization of hiv 1 in proteinsbáo cáo khoa học y họcbáo cáo y tế học đườngmẫu báo cáo y tế học đườngbáo cáo y tế học đường cuối nămbáo cáo y tế học đường năm 2012báo cáo y tế học đường trường mầm nonbiểu mẫu báo cáo y tế trường họcbáo cáo y tế học đường trường tiểu họcBáo cáo thực tập tại nhà thuốc tại Thành phố Hồ Chí Minh năm 2018Báo cáo quy trình mua hàng CT CP Công Nghệ NPVchuyên đề điện xoay chiều theo dạngNghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDEPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhTrả hồ sơ điều tra bổ sung đối với các tội xâm phạm sở hữu có tính chất chiếm đoạt theo pháp luật Tố tụng hình sự Việt Nam từ thực tiễn thành phố Hồ Chí Minh (Luận văn thạc sĩ)Thơ nôm tứ tuyệt trào phúng hồ xuân hươngTổ chức và hoạt động của Phòng Tư pháp từ thực tiễn tỉnh Phú Thọ (Luận văn thạc sĩ)BT Tieng anh 6 UNIT 2Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtchuong 1 tong quan quan tri rui roGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtTrách nhiệm của người sử dụng lao động đối với lao động nữ theo pháp luật lao động Việt Nam từ thực tiễn các khu công nghiệp tại thành phố Hồ Chí Minh (Luận văn thạc sĩ)BÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘI