Báo cáo khoa học: Hexadecylphosphocholine inhibits phosphatidylcholine biosynthesis and the proliferation of HepG2 cells pot

... biosynthesis of PC in HepG2 cells at the level of CTactivity. It interferes with the translocation process of CTfrom the cytosol to the membrane and ﬁnally leads to aninhibition of the biosynthesis ... nonviable cells, as measured by the trypan blue exclusion assay and LDH release into the medium.Inhibition of PC biosynthesis by HePCWe examined the effect of HePC on the biosynthesis of PCby ... Hexadecylphosphocholine inhibits phosphatidylcholine biosynthesis and the proliferation of HepG2 cells Jose´M. Jime´nez-Lo´pez, Marı´a P. Carrasco, Josefa L. Segovia and Carmen...

Tài liệu Báo cáo khoa học: Local stability identiﬁcation and the role of a key aromatic amino acid residue in staphylococcal nuclease refolding pdf

... structure of the protein. Figure 2 shows the ﬂuorescence spectra of wild-type SNase and the fourmutants E142O, K133A, W140A and W140O. The ﬂuorescence spectra of E142O and K133A are similarto that of ... analysis of protein unfolding The DSC curves of the wild-type protein and the mutants K133A, E142O, W140A and W140O areshown in Fig. 3, with their thermodynamic parameterssummarized in Table 1. The ... melting curves of wild-type and mutants of SNase. DSC curves of ﬁve proteins [wild-type (WT), W140A,E140O, W140O and K133A]. The curves of W140A and W140Oare nearly linear in terms of intensity....

Báo cáo khoa học: Interaction between Lim15/Dmc1 and the homologue of the large subunit of CAF-1 – a molecular link between recombination and chromatin assembly during meiosis pot

... activity and invades the homologous double-stranded region of the other allele. These steps of homology search and recombination are catalysedby two bacterial RecA homologues, Rad51 and Lim15/Dmc1. ... [8]. The heterodimeric complex of Hop2 and Mnd1 stimulates strand exchange of Lim15/Dmc1[9–11]. The meiosis-speciﬁc proteins Mei5 and Sae3form a complex with Lim15/Dmc1 and are necessaryfor the ... Western analysis of the rat anti-CcCac1L IgG. The cell extract at the mei-otic prophase was examined. (C) Nuclear localization of CcLim15 and CcCac1L in the nuclei of C. cinerea meiotic cells. Meiotic...

Báo cáo khoa học: Local stability identiﬁcation and the role of key acidic amino acid residues in staphylococcal nuclease unfolding ppt

... capacity (DCp) and difference of DCp of mutant from wild-type (%) were determined and the results for the wild-type protein and 20mutants are given in Table 1. The DHcal and Tm of the wild-type ... the addition of only 2.5 protons (datanot shown). Therefore, we hypothesize that pHunfolding SNase is mainly processed by the addition of 2.5 protons and both E75 and E129 are the targets of ... lower than that of the wild-type protein. The thermodynamic parameters of the glycine susti-tution mutants also differed greatly from the wild-typeprotein. The Tmvalues of these mutants ranged...

Báo cáo khoa học: "PARSING VS. TEXT PROCESSING IN THE ANALYSIS OF DICTIONARY DEFINITIONS" pot

... a sample definition and the triples the parser found in it. ABDOMEN 0 1 N THE PART OF THE BODY BETWEEN THE THORAX AND THE PELVIS (THE) pmod (PART) (ABDOMEN 0 1 N) lm (THE) (ABDOMEN 0 1 ... extracted the set of intransitive verb definitions, suspecting that these would be the easiest to work with. This is the smallest of the four major 219 W7 part of speech categories (the others ... definitions and one vocabulary en~r. There were 128 cases of noun followed by an -/n& form; in 116 of these the -/ng form was a participle, otherwise it was the head noun. (The other case...

Báo cáo khoa học: Glucagon-like peptide-2 stimulates the proliferation of cultured rat astrocytes pptx

... the percentage of hypodiploid cells with respectto total cells [31] and the stage of the cell cycle as the percentage of cells in G0/G1 (diploid cells) , G2/M (tetra-ploid cells) , and S (intermediate ... of GLP-2 (1 lM) and fetal bovine serum (10%) on the percentage of cells present in each phase of the cell cycle incomparison with the control cells. The decrease in the number of cell in the ... administration of GLP-2results in a signiﬁcant increase in the number of c-Fos-positive cells in the dorsomedial hypothalamic nucleus of the rat [25] and in the enteric ganglia of the mouse [7],...

Báo cáo khoa học: From meiosis to postmeiotic events: The secrets of histone disappearance potx

... differentiation is the replacement of canoni-cal histones by a variety of histone variants, whichtakes place extensively in meiotic and postmeiotic cells before the replacement of almost all of the histones.Keywordsacetylation; ... because the latter would be the winners of the competition forDNA binding. The generation of mice without TPs did not, there-fore, allow conclusions to be drawn concerning the role of small ... structural and functional analyses, and all of these studies point to the nucleosomes containing thesevariants as being signiﬁcantly less stable than thosecomposed of canonical histones. The only...

Tài liệu Báo cáo khoa học: A systems biology approach for the analysis of carbohydrate dynamics during acclimation to low temperature in Arabidopsis thaliana doc

... Krapp A, Hofmann B, Schafer C & Stitt M (1993) Reg-ulation of the expression of rbcs and other photosyn-thetic genes by carbohydrates – a mechanism for the sink regulation of photosynthesis. ... interconversion rates as a function of time of cold exposure, (b) comparison of the magnitudes of the various interconversion rates, and (c) lineup of the accessions with respect to their metabolic acclimationstrategies. ... thisfunction. The ﬂux rate of CO2into sucrose synthesis(rCO2!Suc) was caclulated as the difference between the rate of net photosynthesis and that of net starch synthesis (unit:C6h)1Æg)1FW):rCO2!Suc¼...

Tài liệu Báo cáo khoa học: a-Conotoxins as tools for the elucidation of structure and function of neuronal nicotinic acetylcholine receptor subtypes doc

... studies. The highselectivity of the a-conotoxins, together with the possibility of obtaining detailed information on their three dimen-sional structures and the relative ease of synthesis, makesthem ... native and heterologously expressed nAChRs The examples presented above clearly demonstrate the usefulness of a-conotoxins in the determination of the structure and function of native nAChRs, and ... domain of each subunit contri-butes to the formation of the hydrophilic pore. ACh binding proteinhas structural and functional homology to the extracellular ligandbinding domain of the nAChR, and...

Tài liệu Báo cáo khoa học: "What lies beneath: Semantic and syntactic analysis of manually reconstructed spontaneous speech" pdf

... Proceedings of the 47th Annual Meeting of the ACL and the 4th IJCNLP of the AFNLP, pages 746–754,Suntec, Singapore, 2-7 August 2009.c2009 ACL and AFNLPWhat lies beneath: Semantic and syntactic ... in the example) between the abandonedthought and its replacement,2. Determination that the abandoned portioncontains unique and preservable content and should be made a new sentence rather ... Detect the referral of “do” to “like video games”3. Make the necessary word reorderings and deletion of the less informative lexemes.These examples show varying degrees of difﬁ-culty for the...

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