Báo cáo khoa học: Hexadecylphosphocholine inhibits phosphatidylcholine biosynthesis and the proliferation of HepG2 cells pot

... biosynthesis of PC in HepG2 cells at the level of CT activity. It interferes with the translocation process of CT from the cytosol to the membrane and finally leads to an inhibition of the biosynthesis ... nonviable cells, as measured by the trypan blue exclusion assay and LDH release into the medium. Inhibition of PC biosynthesis by HePC We examined the...

Ngày tải lên: 17/03/2014, 11:20

... structure of the protein. Figure 2 shows the fluorescence spectra of wild-type SNase and the four mutants E142O, K133A, W140A and W140O. The fluorescence spectra of E142O and K133A are similar to that of ... analysis of protein unfolding The DSC curves of the wild-type protein and the mutants K133A, E142O, W140A and W140O are shown in Fig. 3, with their therm...

Ngày tải lên: 20/02/2014, 01:20

... activity and invades the homologous double- stranded region of the other allele. These steps of homology search and recombination are catalysed by two bacterial RecA homologues, Rad51 and Lim15/Dmc1. ... [8]. The heterodimeric complex of Hop2 and Mnd1 stimulates strand exchange of Lim15/Dmc1 [9–11]. The meiosis-specific proteins Mei5 and Sae3 form a complex with Lim15...

Ngày tải lên: 07/03/2014, 05:20

... capacity (DC p ) and difference of DC p of mutant from wild-type (%) were determined and the results for the wild-type protein and 20 mutants are given in Table 1. The DH cal and T m of the wild-type ... the addition of only 2.5 protons (data not shown). Therefore, we hypothesize that pH unfolding SNase is mainly processed by the addition of 2.5 protons and bot...

Ngày tải lên: 07/03/2014, 21:20

... a sample definition and the triples the parser found in it. ABDOMEN 0 1 N THE PART OF THE BODY BETWEEN THE THORAX AND THE PELVIS (THE) pmod (PART) (ABDOMEN 0 1 N) lm (THE) (ABDOMEN 0 1 ... extracted the set of intransitive verb definitions, suspecting that these would be the easiest to work with. This is the smallest of the four major 219 W7 part of...

Ngày tải lên: 08/03/2014, 18:20

... the percentage of hypodiploid cells with respect to total cells [31] and the stage of the cell cycle as the percentage of cells in G0/G1 (diploid cells) , G2/M (tetra- ploid cells) , and S (intermediate ... of GLP-2 (1 l M ) and fetal bovine serum (10%) on the percentage of cells present in each phase of the cell cycle in comparison with the control...

Ngày tải lên: 17/03/2014, 03:20

... differentiation is the replacement of canoni- cal histones by a variety of histone variants, which takes place extensively in meiotic and postmeiotic cells before the replacement of almost all of the histones. Keywords acetylation; ... because the latter would be the winners of the competition for DNA binding. The generation of mice without TPs did not, there- fo...

Ngày tải lên: 29/03/2014, 08:20

... Krapp A, Hofmann B, Schafer C & Stitt M (1993) Reg- ulation of the expression of rbcs and other photosyn- thetic genes by carbohydrates – a mechanism for the sink regulation of photosynthesis. ... interconversion rates as a function of time of cold exposure, (b) comparison of the magnitudes of the various interconversion rates, and (c) lineup of the accession...

Ngày tải lên: 14/02/2014, 22:20

... studies. The high selectivity of the a-conotoxins, together with the possibility of obtaining detailed information on their three dimen- sional structures and the relative ease of synthesis, makes them ... native and heterologously expressed nAChRs The examples presented above clearly demonstrate the usefulness of a-conotoxins in the determination of the structur...

Ngày tải lên: 19/02/2014, 12:20

... Proceedings of the 47th Annual Meeting of the ACL and the 4th IJCNLP of the AFNLP, pages 746–754, Suntec, Singapore, 2-7 August 2009. c 2009 ACL and AFNLP What lies beneath: Semantic and syntactic ... in the example) between the abandoned thought and its replacement, 2. Determination that the abandoned portion contains unique and preservable content and should...

Ngày tải lên: 20/02/2014, 07:20