Báo cáo khoa học: De-regulation of D-3-phosphoglycerate dehydrogenase by domain removal ppt

... the CLUSTALW program provided by DNA Data Bank of Japan (DDBJ). The sequence data was analyzed using GENETYX software package (Software Development, Tokyo, Japan). The nucleotide sequence data of Cl-idh will ... m L of 100 m M 2-(cyclohexylamino)- ethanesulfonic acid (Ches) buffer (pH 9.0). T he increase of NADPH was detected by absorbance at 340 nm, and one unit of activity was...

Ngày tải lên: 08/03/2014, 10:20

... the two different forms of the complex are regulated by distinct organelle-specific mechanisms. Activity of the mtPDC is regulated by reversible phosphorylation catalyzed by intrinsic kinase and ... level of sensitivity is provided by metabolite control of the kinase activity. The plPDC is not regulated by reversible phosphorylation. Instead, activity is controlled to a large...

Ngày tải lên: 17/03/2014, 09:20

... of the products To determine the structure of the product generated by ADH3 catalysed reduction of GSNO, nano-electrospray mass spectra of the reaction mixture with and without the addition of ... activity of the human form of ADH3 for this compound. Recombi- nant expression and purification of wild-type and mutated forms of the enzyme enabled kinetic characterization and i...

Ngày tải lên: 31/03/2014, 07:20

... consist of repeats of a homologous sequence domain (blue cop- per-binding domain) that shares distant homology to the single -domain cupredoxins [21,22]. The evolution- ary path from a single -domain ... in chimeras as junctions were found: at the limit of domain D2 in LAC131, at the limit of domain D1 in LAC232, and at the position (or nearby) of the cyste- ine residue C22...

Ngày tải lên: 18/02/2014, 06:20

... 6A). Expression of either STE20 or CLA4 from multicopy plasmids did not alter the levels of SE (Fig. 6B). A higher concentration of SE in cla4D cells could be explained by a negative regulation of Are2p ... we demonstrate that the deletion of either STE20 or CLA4 results in increased levels of sterols. In addition, higher concentrations of steryl esters, the stor- age form of...

Ngày tải lên: 18/02/2014, 13:20

... judgments of the clarity of meaning of indivi- dual sentences. The approach through testing of reading comprehension provides a direct test of at least one aspect of the quality of translation. ... according to the Friedman analysis of variance.* No practice effect is apparent for passages translated by humans. T ABLE 2 Mean Number of Errors by Order of Occurren...

Ngày tải lên: 19/02/2014, 19:20

... In general, the action of neokyotorphin in L929 cells appears be similar to that of cAMP. Results Effect of neokyotorphin in the absence of growth factors To test the ability of neokyotorphin to ... The activity of 1 lm neokyotor- phin was inhibited over the whole concentration range of H-89, confirming the involvement of PKA in the effect of neokyotorphin. As for participati...

Ngày tải lên: 07/03/2014, 11:20

... FEBS Characterization of HbpR binding by site-directed mutagenesis of its DNA-binding site and by deletion of the effector domain David Tropel 1 * and Jan R. van der Meer 1,2 1 Process of Environmental ... bind- ing of the R13 region. Both of these patterns contribu- ted to the loss of binding contacts of mutant 72. Construction of a DA HbpR derivative In order to stud...

Ngày tải lên: 07/03/2014, 17:20

... the prediction of the learning curve of an SMT system as a function of available in -domain parallel data, is the objective of this paper. We consider two scenarios, representative of real- istic ... within the range of the set of initial points. However, it does give a measure of the closeness of the curve fit using only the initial points with the gold fit using all the poi...

Ngày tải lên: 07/03/2014, 18:20

... the regulation of apop- tosis [21,22]. Thus, the possibility that the antiviral effect of spermine is mediated by induction of apop- tosis was analyzed. For this purpose, the effect of spermine ... components of the innate host defense, which act through engagement of specific cell surface receptors and trigger the acti- vation of the janus kinase (JAK) ⁄ signal transducer and...

Ngày tải lên: 07/03/2014, 21:20