Báo cáo khoa học: Suppression of urokinase receptor expression by bikunin is associated with inhibition of upstream targets of extracellular signal-regulated kinase-dependent cascade pptx

... 2002 Suppression of uPAR by bikunin (Eur. J. Biochem. 269) 3957 Suppression of urokinase receptor expression by bikunin is associated with inhibition of upstream targets of extracellular signal-regulated ... uPAR possibly through suppression of upstream targets of ERK. Thus, one of the functions of bikunin is the regulation of uPA...

Ngày tải lên: 17/03/2014, 11:20

... fully understood. It is not by suppression of Akt-1 tran- scription, as Akt-1 transcription is independent of PPARc (Fig. 3D). The inhibition or reduction of Akt-1 activity by PPARc agonists has been ... 96 h. Apoptosis was analyzed by AO ⁄ EB or Hoe- chst 33258 (Hoe ⁄ B.F) staining. (C) Quantita- tive analysis of GW9662 inhibition of adipocyte apoptosis. AO ⁄ EB, ap...

Ngày tải lên: 16/02/2014, 09:20

... of the malignant phenotype. Synthesis and expression of sLe a is a relevant issue per se, as it is the epitope of the CA19.9 antigen, sometimes found to be elevated in the serum of patients with ... low expression of b3Gal-T5 transcript as in the parental cell line (Fig. 2A). This indicates that antisense-mediated mechanism of gene suppression does not involve transcri...

Ngày tải lên: 19/02/2014, 12:20

... not strain-specific, but is a function of the mutant IF1 allele. Fig. 6. A model of possible mechanism of suppression of IF1R69 mutant by 23S processing stem mutations. (A) Effect of the mutant IF1R69L ... the 5¢-side of the processing stem, gave rise to rRNA with the majority of termini in the mature form, whereas pD3, with a mutation on the 3¢-side, gave rise rRNA wi...

Ngày tải lên: 06/03/2014, 00:21

... reversed by down-regulation of PTEN (Fig. 3C). P38MAPK and p53 mediate the apoptosis of NIT-1 cells induced by D-glucose We next explored the molecular mechanisms involved in apoptosis of b-cells ... the apoptosis of NIT-1 cells induced by d-glucose. Suppression of NOX2 substantially restores D-glucose-induced dysfunction and apoptosis of NIT-cells To investigate the poten...

Ngày tải lên: 06/03/2014, 02:20

... cells, showing significant expression of endogenous miR- 125a-5p (data not shown), with antisense miR-125a-5p. After culture, angiogenesis was assessed with tube formation assays. Consistent with its potential ... for EGFR signaling in the expression of miRNA listed in Table 1, we blocked EGFR signaling with gefitinib. Gefitinib is an inhibitor of tyrosine kinase that compete...

Ngày tải lên: 07/03/2014, 00:20

... down-regulated cofactor expression in hTERT-HME cells GR affects gene transcription by a mechanism which is enhanced by cofactors. We therefore characterized the effect of GPR30 on the expression of receptors ... the expression of cofactor were due to GPR30 protein expression. Discussion Molecular discrimination of cells differing in their sensitivity to steroids is es...

Ngày tải lên: 16/03/2014, 18:20

... changed by any of the four types of CRLPs. Effect of the fatty acid composition of CRLPs on COX-2 mRNA expression The effect of CRLPs of varying fatty acid composition on expression of COX-2, ... several isoforms of IjB exist, the most predominant is IjBa [15]. Phosphorylation of IjB by upstream kinases results in its Lys48-linked polyubiquitylation and degradati...

Ngày tải lên: 23/03/2014, 04:20

... binding analysis verified two receptor populations with distinct binding characteristics. The high-affinity population (K d1 and B max1 ) is consistent with the known binding characteristics of the C5aR and ... Surprisingly, substituting the lipid-facing residues of any of the TMs does not significantly alter oligomerization of the C5aR, as assessed by BRET. This is an import...

Ngày tải lên: 23/03/2014, 04:21

... levels of the expression of each cDNA and of the reliability (Present/Absent call) were calculated with software ( AFFYMETRIX MICROARRAY SUITE ver.4.0). For comparisons of the expression level of ... TR is expressed. As well as treatment of the HeLaTR cells with T3, overexpression of 4-1BB in the HeLaTR cells led to the activation of caspases. Activation of caspase...

Ngày tải lên: 23/03/2014, 18:20