Báo cáo khoa học: Characterization of scorpion a-like toxin group using two new toxins from the scorpion Leiurus quinquestriatus hebraeus doc

... Purification of the toxins Lqh6 (A) and Lqh7 (B) from the venom of the scorpion Leiurus quinquestriatus hebraeus (Lqh). Fig. S2. Amino acid sequences of toxins Lqh6 and Lqh7. Ó FEBS 2002 Two new a-like toxins ... characterization of two new toxins, desig- nated Lqh6 and Lqh7, from the venom of the yellow scorpion Leiurus quinquestriatus...

Ngày tải lên: 17/03/2014, 11:20

... the text and in Fig. 5B. n, Number of [A] tested; k 2 ⁄ k 1 , the value of the intercept in the Scrutton and Utter plot, where k 2 and k 1 are the rate constants of the inhibition of either the ... k A and k 0 are the inactivation rate constants either in the presence or in the absence of various concentrations of a ligand A, k 2 and k 1 are the rate constant...

Ngày tải lên: 20/02/2014, 01:20

... that the presence of the ARS in the 5¢-UTR of an mRNA stalls the 40S ribosomal subunit before the ARS. Therefore, the ARS-containing mRNA is arrested at the translation initiation step [13]. As the TOP ... in the presence of the ARS. To further elucidate whether the effect on the b-gal level was due to changes in the abundance of the cognate mRNA, we measur...

Ngày tải lên: 18/02/2014, 12:20

... guarantee the presence of a perfect Gauss- ian shape. The second hypothesis necessary to perform Student’s t-test requires that the variance of the two samples is equal. By estimating the variance from ... 5A,B shows the ML signal intensities of 1D spectra from the two cell lines run at different days. Samples of cells grown under the same conditions were irrad...

Ngày tải lên: 18/02/2014, 13:20

... characterized by the degeneration of larval tissues, such as the velum and the foot, and the remodelling of larval tissues to produce adult tissues (i.e. the development of the gills and the production of ... gigas. In this article, we report the characterization of a new CLP, named Cg-Clp2, from the oyster C. gigas. The tissue distribution and temporal pat...

Ngày tải lên: 19/02/2014, 00:20

... (Roche). The threshold cycle was calculated using the sequence detection software as the cycle number at which the fluorescence of the reporter dye crossed the threshold in log-linear range of PCR. The ... numbers over the diagrams indicate the position of the corresponding nucleotides relative to the translation start site, and the numbers in parentheses below...

Ngày tải lên: 19/02/2014, 06:20

... of the first 88 amino acids of PPI1 may participate in the interaction with the H + -ATPase and makes PPI1 588 His 6 a suitable tool to study the mechanism of action of PPI1. The analysis of the ... 14-3-3s. Results The C-terminus of isoform 1 of the PM H + -ATPase of A. thaliana (AHA1) interacts with the first 88 amino acids of PPI1 [22], indicating that th...

Ngày tải lên: 19/02/2014, 07:20

... (1988) Nucleotide sequence of the gene for aqualysin I (a ther- mophilic alkaline serine protease) of Thermus aquaticus YT-1 and characteristics of the deduced primary struc- ture of the enzyme. Eur J ... or two extended C-terminal region(s) of the peptidase sequences are common within the bacterial subgroup of the proteinase K fam- ily. Database search on the seco...

Ngày tải lên: 19/02/2014, 07:20

... together in the stationary phase of yeast cells [8] and the result of two- hybrid analysis [23], they may form a complex. To assess these possibilities, we embarked on a characterization of the two ... complement the defect. The relationship of all of these genes remains to be clarified. Expression and purification of Sno1p and Snz1p In light of the similarity in...

Ngày tải lên: 19/02/2014, 12:20

... also play a critical role in the assembly of the preinitiation complex within TATA-less promoters [47]. The AP-1 complex is com- prised of a group of proteins encoded by the jun (c-Jun, JunB, JunD) ... (2002) Characteri- zation of the 5¢ untranslated region of alpha and beta isoforms of the human thromboxane A2 receptor (TP). Differential promoter utilization by the...

Ngày tải lên: 19/02/2014, 16:20