Báo cáo khoa học: Maturation of Pichia pastoris-derived recombinant pro-Der p 1 induced by deglycosylation and by the natural cysteine protease Der p 1 from house dust mite doc
Báo cáo khoa học: Maturation of Pichia pastoris-derived recombinant pro-Der p 1 induced by deglycosylation and by the natural cysteine protease Der p 1 from house dust mite doc
... rpro- Der p 1 (SMD 116 8 h); lane 3, Endo H-treated rpro -Der p 1 (SMD 116 8h); lane 4, nDer p 1 . (C) Lane 1, rpro -Der p 1 (X-33); lane 2, rpro -Der p 1 (SMD 116 8h); lane 3, Endo H-treated rpro -Der p 1; lane ... Maturation of Pichia pastoris -derived recombinant pro -Der p 1 induced by deglycosylation and by the natural cysteine...
Ngày tải lên: 17/03/2014, 11:20
Báo cáo khoa học: "Effect of intern’s consecutive work hours on safety, medical education and professionalism"
... clarification of the power, sample size, and statistical approach descriptions that appeared in our recent Journal Club review [1] of their studies examining the effect of reducing interns work hours in the ... http://ccforum.com/content/9/5/528 We agree with the recommendation that further research should study the effects of sleep deprivation and work schedule interven...
Ngày tải lên: 25/10/2012, 10:45
Tài liệu Báo cáo khoa học: Transcription of individual tRNA1Gly genes from within a multigene family is regulated by transcription factor TFIIIB pdf
... TBP, Brf2 and Bdp1 required for transcription of U6-type RNA pol III promoters) [14 ] and TFIIIBb (comprising TBP, Brf1 and Bdp1 required for transcription of tRNA and VA1-type RNA pol III promoters) ... tRNA Gly 1 -6,7 (at position )26 with respect to the +1 of tRNA) proper positioning of the TATA sequences appeared necessary for the formation of stable complexes....
Ngày tải lên: 20/02/2014, 02:21
Báo cáo khoa học: Suppression of NADPH oxidase 2 substantially restores glucose-induced dysfunction of pancreatic NIT-1 cells doc
... p3 8MAPK and p5 3 mediate the apoptosis of NIT -1 cells induced by d-glucose. Exposure to d-glucose for 48 h substantially stimulated phosphorylation of p3 8MAPK and p5 3, accompanied by activation of ... induced by d-glucose. It has been reported that p3 8MAPK is activated by dual phosphorylation of 18 0Thr and 18 2Tyr residues, and p5 3 is activated b...
Ngày tải lên: 06/03/2014, 02:20
Báo cáo khóa học: Characterization of Mesorhizobium huakuii lipid A containing both D-galacturonic acid and phosphate residues ppt
... 3-OH-i -13 :0 16 40 .13 7 B 1 + 14 81 – – – 1 1 1 2 1 1 81 P- DAG, 1 · 3-OH-20:0, 1 · 3-OH-i -13 :0, 27-OH-28:0, 20:0 14 81. 276 B 1 + 11 87 – – – 1 1 1 2 1 0 61 P- DAG, 1 · 3-OH-20:0, 1 · 3-OH-i -13 :0, 27-OH-28:0 11 86.753 Unphosphorylated ... Mesorhizobium huakuii IFO 15 243 T . The signal at 1. 71 p. p.m. (A) recorded at pH 7.3, shifted to 4. 71 p...
Ngày tải lên: 07/03/2014, 15:20
Báo cáo khoa học: Evaluation of potential regulatory elements identi®ed as DNase I hypersensitive sites in the CFTR gene doc
... intron 2 (P1 .8, ac00 011 1: 46769±48558), 10 (B1.5, ac00 011 1: 11 1755± 11 3258 and H2 .1, ac00 011 1: 12 0380 12 2527), 16 17 a Correspondence to A. Harris, Paediatric Molecular Genetics, Institute of Molecular ... F34L; intron 10 (17 16 + 13 .2 kb, + 13 .7 k b and + 23 kb), 11 6 /11 7 and BT1.2; i ntron 16 and 17 a ( 312 0 + 3kb and 32 71 + 0.7 kb) E1.9 ; i ntron 2...
Ngày tải lên: 08/03/2014, 10:20
Báo cáo khoa học: Isolation of a putative peroxidase, a target for factors controlling foot-formation in the coelenterate hydra docx
... Transcripts of ppod2 are abundant in the whole animal with the exception of the hypostome, the tentacles, and the foot; the expression of ppod1 matches exactly the localization of the foot-specific peroxidase. Keywords: ... thus confirming the identity of the cloned peroxidase (Fig. 8). Localization of ppod2 and ppod1 in hydra tissue The ppod1 and ppod2 e...
Ngày tải lên: 08/03/2014, 10:20
Tài liệu Báo cáo khoa học: Impact of the native-state stability of human lysozyme variants on protein secretion by Pichia pastoris doc
... Da 10 + 9+ B A 11 + 12 + 10 + 9+ 8+ 12 + 8+ W64R lysozyme MW (obs): 14 662.2 ± 1. 5 Da MW (calc): 14 662.6 Da 14 kDa 13 + 7+ 11 00 13 00 15 00 17 00 19 00 210 0 0 % m/z 10 0 15 00 17 00 19 0 013 0 011 00 210 0 0 10 0 % m/z 12 1 2 Fig. ... 95 S80A 1. 06 ± 0 .15 10 .0 ± 2.0 85 I89V 0. 81 ± 0 .14 8.2 ± 1. 0 85 V93A 0.57 ± 0 .17 8 .1 ± 0.8 95 WT 1. 0 8.3 ± 1. 1 10...
Ngày tải lên: 19/02/2014, 08:20
Báo cáo khoa học: Suppression of a cold-sensitive mutant initiation factor 1 by alterations in the 23S rRNA maturation region pdf
... pBR322-derived [35] pD1 pKK3535 but suppressor of infA R69L This work pD3 pKK3535 but suppressor of infA R69L This work pD6 pKK3535 but suppressor of infA R69L This work pTrc99a Amp, pUC-derived ... & Noller HF (19 81) Construction and fine mapping of recombinant plasmids containing the rrnB ribosomal RNA operon of E. coli. Plasmid 6, 11 2– 11 8. 36 Link AJ, Phillips D &a...
Ngày tải lên: 06/03/2014, 00:21
Báo cáo khoa học: Kinetics of the inhibition of neutrophil proteinases by recombinant elafin and pre-elafin (trappin-2) expressed in Pichia pastoris ppt
... portion of elafin and trappin-2. Primers 1 and 2 fuse the trappin-2 and elafin mature protein, respectively, immediately downstream of the a-peptide sequence and downstream of the Lys-Arg dipep- tide ... 10 )4 (0.3 ± 0 .1) 10 )10 a Proteinase 3 (3.3 ± 0.03) 10 6 (4 ± 0.3) 10 )4 (1. 2 ± 0 .1) 10 )10 a (2 ± 0 .1) 10 6 (3.7 ± 1. 1) 10 )4 (1. 8 ± 0.6) 10...
Ngày tải lên: 07/03/2014, 15:20