Báo cáo khoa học: Maturation of Pichia pastoris-derived recombinant pro-Der p 1 induced by deglycosylation and by the natural cysteine protease Der p 1 from house dust mite doc

... rpro- Der p 1 (SMD 116 8 h); lane 3, Endo H-treated rpro -Der p 1 (SMD 116 8h); lane 4, nDer p 1 . (C) Lane 1, rpro -Der p 1 (X-33); lane2, rpro -Der p 1 (SMD 116 8h); lane 3, Endo H-treated rpro -Der p 1; lane ... Maturation of Pichia pastoris-derived recombinant pro -Der p 1 induced by deglycosylation and by the natural cysteine protease Der p 1 from house dust mite Erica van Oort, Pleuni G. ... recombinant pro -Der p 1 facilitated by puri®ed nDer p 1. Lane 1, 0 h rpro -Der p 1; lane 2, +0.37 lg nDer p 1; lane 3, +0.74 lg nDer p 1; lane 4, +1. 48 lg nDer p 1; lane 5, +2.96 lgnDer p 1; lane...

Báo cáo khoa học: "Effect of intern’s consecutive work hours on safety, medical education and professionalism"

... clarification of the power, sample size, and statistical approach descriptions thatappeared in our recent Journal Club review [1] of theirstudies examining the effect of reducing interns work hours in the ... http://ccforum.com/content/9/5/528We agree with the recommendation that further researchshould study the effects of sleep deprivation and workschedule interventions on the performance of upper-levelresidents ... upper-levelresidents and other medical staff across a variety of disciplines. We likewise agree that optimizing patient hand-offs, medical education, and trainees’ sense of professionalism should be priorities...

Tài liệu Báo cáo khoa học: Transcription of individual tRNA1Gly genes from within a multigene family is regulated by transcription factor TFIIIB pdf

... TBP, Brf2 and Bdp1 required fortranscription of U6-type RNA pol III promoters) [14 ] and TFIIIBb (comprising TBP, Brf1 and Bdp1required for transcription of tRNA and VA1-typeRNA pol III promoters) ... tRNAGly 1 -6,7(at position )26 with respect to the +1 of tRNA)proper positioning of the TATA sequences appearednecessary for the formation of stable complexes. The mere presence of the TATA ... right-hand side of the upper panel.Regulation of pol III transcription A. Parthasarthy and K. P. Gopinathan 519 2 FEBS Journal 272 (2005) 519 1–5205 ª 2005 FEBSTranscription of tRNAGly 1 -6,7 (poorly...

Báo cáo khoa học: Suppression of NADPH oxidase 2 substantially restores glucose-induced dysfunction of pancreatic NIT-1 cells doc

... p3 8MAPK and p5 3 mediate the apoptosis of NIT -1 cells induced by d-glucose.Exposure to d-glucose for 48 h substantially stimulatedphosphorylation of p3 8MAPK and p5 3, accompanied by activation of ... induced by d-glucose. It hasbeen reported that p3 8MAPK is activated by dualphosphorylation of 18 0Thr and 18 2Tyr residues, and p5 3 is activated by phosphorylation of 15 Ser residues.Phosphorylation ... that p3 8MAPK and p5 3 mediate the apoptosis of NIT -1 cells induced by d-glucose.Suppression of NOX2 substantially restoresD-glucose -induced dysfunction and apoptosis of NIT-cellsTo investigate the...

Báo cáo khóa học: Characterization of Mesorhizobium huakuii lipid A containing both D-galacturonic acid and phosphate residues ppt

... 3-OH-i -13 :0 16 40 .13 7B 1 + 14 81 – – – 1 1 1 2 1 1 81 P- DAG, 1 · 3-OH-20:0, 1 · 3-OH-i -13 :0,27-OH-28:0, 20:0 14 81. 276B 1 + 11 87– – – 1 1 1 2 1 0 61 P- DAG, 1 · 3-OH-20:0, 1 · 3-OH-i -13 :0,27-OH-28:0 11 86.753Unphosphorylated ... Mesorhizobiumhuakuii IFO 15 243T. The signal at 1. 71 p. p.m. (A) recorded at pH 7.3,shifted to 4. 71 p. p.m. (B) at pH 10 .60, and represents ester-boundmonophosphate residue. 13 12 A. Choma and P. Sowinski ... 3-OH-i -13 :0,27-OH-28:0, 20:0 19 82.896B 1 + 14 00– – – 1 0 1 2 1 1 81 DAG2 · 3-OH-20:0,2 · 3-OH-i -13 :0,27-OH-28:0, 20:0 14 01. 297B 1 + 11 08– – – 1 0 1 2 1 0 61 DAG2 · 3-OH-20:0,2 · 3-OH-i -13 :0,27-OH-28:0 11 06.774 13 14...

Báo cáo khoa học: Evaluation of potential regulatory elements identi®ed as DNase I hypersensitive sites in the CFTR gene doc

... intron 2 (P1 .8,ac00 011 1: 46769±48558), 10 (B1.5, ac00 011 1: 11 1755± 11 3258 and H2 .1, ac00 011 1: 12 0380 12 2527), 16 17 aCorrespondence to A. Harris, Paediatric Molecular Genetics, Institute of Molecular ... F34L; intron 10 (17 16 + 13 .2 kb, + 13 .7 k b and + 23 kb), 11 6 /11 7 and BT1.2; i ntron 16 and 17 a( 312 0 + 3kb and 32 71 + 0.7 kb) E1.9 ; i ntron 20 (4005 +4 kb) EB1.4 and intron 21 (4095 + 7.2 ... ndrug-treated and control samples on Southern blots of chromatin. The intensity of the signal derived from the genomic band and the DHS-derived band were determinedusing a phosphorimager and IMAGEQUANT5 .12 ...

Báo cáo khoa học: Isolation of a putative peroxidase, a target for factors controlling foot-formation in the coelenterate hydra docx

... Transcripts of ppod2 are abundantin the whole animal with the exception of the hypostome, the tentacles, and the foot; the expression of ppod1 matchesexactly the localization of the foot-speciﬁc peroxidase.Keywords: ... thusconﬁrming the identity of the cloned peroxidase (Fig. 8).Localization of ppod2 and ppod1 in hydra tissue The ppod1 and ppod2 expression patterns were analyzed by in situ hybridization. These experiments ... sequences of the newly obtainedPCR-fragments yielded two different, highly homologousclones, which we designated ppod1 and ppod2 (pp standingfor putative peroxidase). The lengths of ppod2 and ppod1...

Tài liệu Báo cáo khoa học: Impact of the native-state stability of human lysozyme variants on protein secretion by Pichia pastoris doc

... Da 10 +9+BA 11 + 12 + 10 +9+8+ 12 +8+W64R lysozymeMW (obs): 14 662.2 ± 1. 5 DaMW (calc): 14 662.6 Da 14 kDa 13 +7+ 11 00 13 00 15 00 17 00 19 00 210 00%m/z 10 0 15 00 17 00 19 0 013 0 011 00 210 00 10 0%m/z 12 1 2Fig. ... 95S80A 1. 06 ± 0 .15 10 .0 ± 2.0 85I89V 0. 81 ± 0 .14 8.2 ± 1. 0 85V93A 0.57 ± 0 .17 8 .1 ± 0.8 95WT 1. 0 8.3 ± 1. 1 10 0aValues reported are the yield per OD600 of 1. 0 for each variant rel-ative to the ... 711 –720 ª 2006 The Authors Journal compilation ª 2006 FEBS 711 has an allele frequency of 5% in the British popula-tion, and has been identiﬁed in 12 % of the whiteCanadian population [18 ,19 ].Recombinant...

Báo cáo khoa học: Suppression of a cold-sensitive mutant initiation factor 1 by alterations in the 23S rRNA maturation region pdf

... pBR322-derived [35]pD1 pKK3535 but suppressor of infA R69LThis workpD3 pKK3535 but suppressor of infA R69LThis workpD6 pKK3535 but suppressor of infA R69LThis workpTrc99a Amp, pUC-derived ... & Noller HF (19 81) Construction and ﬁnemapping of recombinant plasmids containing the rrnBribosomal RNA operon of E. coli. Plasmid 6, 11 2– 11 8.36 Link AJ, Phillips D & Church GM (19 97) ... Leif.Isaksson@gmt.su.se(Received 24 January 2 011 , revised 1 March2 011 , accepted 14 March 2 011 )doi :10 .11 11/ j .17 42-4658.2 011 .08099.xGenetic selection has been used to isolate second-site suppressors of adefective cold-sensitive...

Báo cáo khoa học: Kinetics of the inhibition of neutrophil proteinases by recombinant elaﬁn and pre-elaﬁn (trappin-2) expressed in Pichia pastoris ppt

... portion of elaﬁn and trappin-2.Primers 1 and 2 fuse the trappin-2 and elaﬁn matureprotein, respectively, immediately downstream of the a-peptide sequence and downstream of the Lys-Arg dipep-tide ... 10 )4(0.3 ± 0 .1) 10 )10 aProteinase 3 (3.3 ± 0.03) 10 6(4 ± 0.3) 10 )4 (1. 2 ± 0 .1) 10 )10 a(2 ± 0 .1) 10 6(3.7 ± 1. 1) 10 )4 (1. 8 ± 0.6) 10 )10 aPancreatic elastase ND ND (7.5 ± 1. 5) 10 )10 ND ND ... related yeast protein and wasnot further characterized. To limit the appearance of the 13 kDa clipped form of trappin-2 for the large-scaleproduction of trappin-2, the duration of fermentation...

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