Báo cáo khoa học: Substrate recognition and ®delity of strand joining by an archaeal DNA ligase docx

... Substrate recognition and ®delity of strand joining by an archaeal DNA ligase Masaru Nakatani 1,2 , Satoshi Ezaki 1,2 , Haruyuki Atomi 1,2 and Tadayuki Imanaka 1,2 1 Department of Synthetic ... release of free AMP from the DNA adenylate [2,5,6,13]. Catalytic activity of DNA ligase is dependent on appropriate divalent cations and DNA substrates. In ge...

Ngày tải lên: 17/03/2014, 11:20

... after (Fig. 2B,C) addition of the enzyme. Table 2. Kinetic parameters and DDGà for hydrolysis of isomaltose and p-nitrophenyl-a- D -glucopyranoside, and mono-deoxy analogs of methyl a-isomaltoside ... was found in isomaltose hydrolysis by glucoamylase, which depended on enzyme± substrate transition-state interactions with OH-4 and -3 of an energy of 16.5 and 8.6 kJám...

Ngày tải lên: 23/03/2014, 21:21

... Eckhardtà, Emanuela Borter§, Patrick Spielmann and Roland H. Wenger Institute of Physiology and Zu ¨ rich Center for Integrative Human Physiology, University of Zu ¨ rich, Switzerland Keywords metabolism; ... against S6 and the GST-tag. (C) Immunoblot analysis of the phosphorylation status of p70S6K and S6 in Paskin + ⁄ + and Paskin ) ⁄ ) MEFs. (D) Immu- noblot analysis of...

Ngày tải lên: 06/03/2014, 00:21

... (5¢-TTTGCGGCCGCTCATAGACACAAATTGCCGG Fig. 7. MALDI-TOF MS analysis of peaks A (A) and B (B) in Fig. 6. The peaks were fractionated and analyzed by MALDI-TOF MS. Peak A included oligosaccharides of DP 16–18, and peak B included oligosaccharides ... Reading, PA, USA). HPLC and MALDI-TOF MS analysis The reaction products of TXG and XXXGXXXG gener- ated by Xgh74B treatment were...

Ngày tải lên: 30/03/2014, 03:20

... unlabeled wild-type as well as by two mutant variants of PCSK9 and LDL, but not by an unrelated ligand, suggesting that they all compete with [ 125 I]TC-PCSK9 for the same receptor. Linear [27–29] and curvilinear ... or with unlabeled ligand or medium (pH 6.2), and dissociation of specifically bound ligands was followed as a function of time. Data are pre- sented as percentag...

Ngày tải lên: 14/02/2014, 14:20

... expression of T-bet has been seen within a wide variety of tissues and cells in the Ginbuna crucian carp [27] and, in mammals, in the lung tissue of mouse and spleen and thymus of human and mouse ... [44]. The gene organizations of zebrafish t-bet, stat6 and foxp3 were also determined and found to be very simi- lar to their human homologues. Both the human and zebrafish...

Ngày tải lên: 16/02/2014, 09:20

... muscle with organ, but without fat, of WT and H-PGDS TG mice (n = 6). Continuous dissections of mouse fat and bone in the whole body were quantified by use of a micro-CT scanner and LATHETA software (Aloka). ... the 32 P-labeled full-length cDNA for human H-PGDS. (C) Immunohistochemical analysis of transgene expression in the liver and WAT. Paraffin sections of liver and W...

Ngày tải lên: 16/02/2014, 09:20

... Expression of GmPDIL-3a and GmPDIL-3b in soybean tissues. (A) Purified recombinant GmPDIL-3a (lanes 1 and 4), GmPDIL-3b (lanes 2 and 5) and proteins extracted from the cotyle- don (lane 3) were analyzed ... (80 mg bean) (lane 1), root (lane 2), stem (lane 3), leaf (lane 4) and flower (lane 5) were analyzed by western blot using anti-GmPDIL-3a serum. A B Fig. 4. Localization of G...

Ngày tải lên: 18/02/2014, 11:20

... addition to antiplatelet and coagu- lant actions, PUFAs also inhibit lipogenesis and lower tissue and plasma triglyceride levels through inhibition of SREBP-1c. The molecular mechanisms by which PUFAs ... activate, and PUFAs suppress, SREBP- 1c, leading to and protecting from hepatic insulin resistance and pancreatic b-cell insulin secretion, respectively [39]. FA, fatty acid....

Ngày tải lên: 18/02/2014, 13:20

... 40G14, 9E14, 51H3, and 6H19. The BAC DNA was isolated, and the presence of the SmSmad1B sequence was confirmed by PCR and by sequencing the BAC DNA. Exon–intron sites were located by aligning the cDNA sequence ... Drosophila MAD and the vertebrate Smad1, Fig. 1. Structure of the SmSmad1B gene, cDNA and protein. (A) Schematic representations of the SmSmad1B gene, cDNA...

Ngày tải lên: 18/02/2014, 16:20